王五香^1,2,
高丹¹,
廖芬¹,
周芙蓉¹,
封少龙^1,,
1. 南华大学公共卫生学院, 衡阳 421001;
2. 南华大学图书馆, 衡阳 421001

作者简介: 王五香(1970-),女,中级职称,研究方向为环境污染物的生态与健康影响机制,E-mail:wxwang_feng@126.com.

通讯作者: 封少龙,slfeng71@usc.edu.cn

基金项目: 湖南省教育厅重点项目(12A118)
南华大学归国****启动基金(2012XQD44)
南华大学研究生教育教改项目(2014JG015)


中图分类号: X171.5

New Views on the Methodology of Micronucleus Assay with Vicia faba Root Tip Cells

Wang Wuxiang^1,2,
Gao Dan¹,
Liao Fen¹,
Zhou Furong¹,
Feng Shaolong^1,,
1. The School of Public Health, University of South China, Hengyang 421001, China;
2. The Library, University of South China, Hengyang 421001, China

Corresponding author: Feng Shaolong,slfeng71@usc.edu.cn

CLC number: X171.5

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摘要:虽然蚕豆根尖微核技术已是一个成熟规范的检测污染物遗传毒性的方法,但其中仍涉及一些重要的方法学问题值得深入探讨和研究。本文在图示正确观测蚕豆根尖细胞内微核和染色体畸变,如染色体断裂、丢失及染色体桥等的基础上,提出应该对蚕豆根尖分裂相细胞及其染色体畸变进行观测,以便更加准确、细致地反映污染物作用的剂量-效应关系和分子机制。同时还就该方法的其他重要问题提出了自己的观点,供同行们深入探讨和研究,使之不断完善,更好地服务于环境监测和风险评价等领域。
关键词: 蚕豆/
微核/
染色体畸变/
形态学/
方法学

Abstract:Although the micronucleus assay with Vicia faba root tip cells is a canonical way to detect the genotoxicity of environmental pollutants, there are still some important issues that are worth in-depth discussion and investigation in its methodology. After illustrating how to correctly observe the micronucleus and chromosome aberrations, including chromosome fragment, chromosome loss and chromosome bridge, in the Vicia faba root tip cells we suggested that the dividing-phase cells and their chromosome aberrations should been taken account of in this assay, in order to more precisely and delicately determine the dose-effect relationship and potential molecular mechanisms than before. Other important issues about its methodology were also presented for our colleagues to discuss and explore deeply. The aim is to improve this assay to better serve the environmental monitoring and risk assessment.
Key words:Vicia faba/
micronucleus/
chromosome aberration/
morphology/
methodology.