王维斌，放射医学系预聘制助理教授，博士生导师
联系方式：
E-mail：weibinwang@bjmu.edu.cn
科研经历：
学士，首都医科大学，医学实验学系
硕士&博士，北京大学医学部，生物化学与分子生物学系
博士后，耶鲁大学，分子生物物理与生物化学系
研究方向：
王维斌博士毕业于北京大学医学部，师从张晓伟教授和童坦君院士，参与研究的课题涉及细胞衰老和肿瘤抑制等领域，具体研究细胞衰老相关基因在转录水平的调控、细胞衰老相关蛋白在翻译后修饰水平的调控，以及抑癌因子的翻译后修饰与肿瘤之间的联系等。王维斌博士在美国耶鲁大学完成了博士后的科研工作，其合作导师是DNA损伤修复领域著名学者Patrick Sung教授，长期从事DNA损伤修复与肿瘤发生和治疗的研究。随后，王维斌博士以独立PI的身份加入北京大学医学部放射医学系，该课题组运用生物化学结合分子生物学和细胞生物学的手段，着重研究DNA损伤的修复机制及其在细胞衰老或肿瘤抑制中的作用，具体开展的研究课题包括：
1）疾病相关核酸酶复合体MRN-CtIP参与同源重组修复DNA损伤的机制。
2）CtIP在磷酸化或类泛素化等翻译后修饰水平的调控。
3）53BP1新型结合因子对MRN/EXO1/DNA2介导的DNA末端切割的负调控。
4）DNA末端切割的重建与BRCA缺失肿瘤细胞的耐药性之间的联系。
5）抑癌因子PALB2-MRG15-RAD51同源物与DNA损伤修复。
6）DNA损伤关键修复因子在细胞衰老中的作用等。
发表文章：
1. Xue, C., Wang, W., Crickard, J. B., Moevus, C. J., Kwon, Y., Sung, P., and Greene, E. C. (2019) Regulatory control of Sgs1 and Dna2 during eukaryotic DNA end resection. Proc. Natl. Acad. Sci. U. S. A. (accepted)
2. Wang, W., Daley, J. M., Kwon, Y., Xue, X., Krasner, D. S., Miller, A. S., Nguyen, K. A., Williamson, E. A., Shim, E. Y., Lee, S. E., Hromas, R., and Sung, P. (2018) A DNA nick at Ku-blocked double-strand break ends serves as an entry site for exonuclease 1 (Exo1) or Sgs1-Dna2 in long-range DNA end resection. Journal of Biological Chemistry 293, 17061-17069.
3. Cassani, C., Gobbini, E., Vertemara, J., Wang, W., Marsella, A., Sung, P., Tisi, R., Zampella, G., and Longhese, M. P. (2018) Structurally distinct Mre11 domains mediate MRX functions in resection, end-tethering and DNA damage resistance. Nucleic Acids Research 46, 2990-3008.
4. Gobbini, E., Cassani, C., Vertemara, J., Wang, W., Mambretti, F., Casari, E., Sung, P., Tisi, R., Zampella, G., and Longhese, M. P. (2018) The MRX complex regulates Exo1 resection activity by altering DNA end structure. EMBO Journal 37, e98588.
5. Wang, W., Daley, J. M., Kwon, Y., Krasner, D. S., and Sung, P. (2017) Plasticity of the Mre11-Rad50-Xrs2-Sae2 nuclease ensemble in the processing of DNA-bound obstacles#. Genes & Development 31,c2331-2336.
# highlighted in an Outlook article: Gnugge, R., and Symington, L. S. (2017) Keeping it real: MRX-Sae2 clipping of natural substrates. Genes & Development 31, 2311-2312.
6. Zhao, W., Steinfeld, J. B., Liang, F., Chen, X., Maranon, D. G., Jian Ma, C., Kwon, Y., Rao, T., Wang, W., Sheng, C., Song, X., Deng, Y., Jimenez-Sainz, J., Lu, L., Jensen, R. B., Xiong, Y., Kupfer, G. M., Wiese, C., Greene, E. C., and Sung, P. (2017) BRCA1-BARD1 promotes RAD51-mediated homologous DNA pairing. Nature 550, 360-365.
7. Daley, J. M., Jimenez-Sainz, J., Wang, W., Miller, A. S., Xue, X., Nguyen, K. A., Jensen, R. B., and Sung, P. (2017) Enhancement of BLM-DNA2-Mediated Long-Range DNA End Resection by CtIP. Cell Reports 21, 324-332.
8. Chen, Y., Pan, K., Wang, P., Cao, Z., Wang, W., Wang, S., Hu, N., Xue, J., Li, H., Jiang, W., Li, G., and Zhang, X. (2016) HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis. Journal of Biological Chemistry 291, 12688-12705.
9. Cassani, C., Gobbini, E., Wang, W., Niu, H., Clerici, M., Sung, P., and Longhese, M. P. (2016) Tel1 and Rif2 Regulate MRX Functions in End-Tethering and Repair of DNA Double-Strand Breaks. PLOS Biology 14, e**.
10. Wang, W., Chen, Y., Wang, S., Hu, N., Cao, Z., Wang, W., Tong, T., and Zhang, X. (2014) PIASxα ligase enhances SUMO1 modification of PTEN protein as a SUMO E3 ligase. Journal of Biological Chemistry 289, 3217-3230.
11. Pan, K., Chen, Y., Roth, M., Wang, W., Wang, S., Yee, A. S., and Zhang, X. (2013) HBP1-mediated transcriptional regulation of DNA methyltransferase 1 and its impact on cell senescence. Molecular and Cellular Biology 33, 887-903.
12. Wang, W., Pan, K., Chen, Y., Huang, C., and Zhang, X. (2012) The acetylation of transcription factor HBP1 by p300/CBP enhances p16INK4A expression. Nucleic Acids Research 40, 981-995.
13. Li, H.#, Wang, W.#, Liu, X., Paulson, K. E., Yee, A. S., and Zhang, X. (2010) Transcriptional factor HBP1 targets p16INK4A, upregulating its expression and consequently is involved in Ras-induced premature senescence. Oncogene 29, 5083-5094. (# first author)