关键词: 相干反斯托克斯拉曼散射/
显微成像/
分辨率
English Abstract
Research on coherent anti-Stokes Raman scattering microscopy
Liu Shuang-Long,Liu Wei,
Chen Dan-Ni,
Qu Jun-Le,
Niu Han-Ben
1.Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education and Guangdong Province, College of Opto-Electronic Engineering, Shenzhen University, Shenzhen 518060, China
Fund Project:Project supported by the National Basic Research Program of China(Grant Nos. 2012CB825802, 2015CB352005), the National Natural Science Foundation of China (Grant Nos. 61235012, 61178080, 11004136), the Special Funds of the Major Scientific Instruments Equipment Development of China (Grant No. 2012YQ15009203), and the Science and Technology Planning Project of Shenzhen, China (Grant Nos. JCYJ20120613173049560, GJHS20120621155433884).Received Date:11 September 2015
Accepted Date:23 November 2015
Published Online:05 March 2016
Abstract:In this paper, we analyze the process of coherent anti-Stokes Raman scattering (CARS) based on quantum theory and set up a traditional point-scanning CARS microscope. With this microscope, high-contrast images of polystyrene microspheres are obtained. By scanning polystyrene beads with 110 nm diameter, we reconstruct the point spread faction (PSF) of the system. And the full width at half maximum (FWHM) of the PSF shows a lateral resolution about 600 nm, which is larger than the theoretical value (～ 300 nm). Therefore, we propose several resolution-improvement approaches, which lay a strong foundation for the realization of nano-CARS microscopy.
Keywords: coherent anti-Stokes Raman scattering/
microscopy/
resolution
