张一鸣^1,2,,
黄咸雨^1,2,3,,,
谢树成^1,2
1. 中国地质大学(武汉)生物地质与环境地质国家重点实验室, 湖北 武汉 430078
2. 中国地质大学(武汉)流域关键带演化湖北省重点实验室, 湖北 武汉 430074
3. 中国地质大学(武汉)地理与信息工程学院, 湖北 武汉 430078

基金项目: 国家自然科学基金项目（批准号：41877317和41830319）、国家自然科学基金创新研究群体项目（批准号：41821001）和高等学校学科创新引智计划项目（批准号：BP0820004）共同资助


详细信息

作者简介: 张一鸣, 男, 28岁, 博士研究生, 第四纪地质学专业, E-mail: yiming.zhang@cug.edu.cn

通讯作者: 黄咸雨, E-mail: xyhuang@cug.edu.cn

中图分类号: P593;P534.63

收稿日期:2020-11-20
修回日期:2021-02-18
刊出日期:2021-07-30

Compound-specific carbon isotope compositions of microbial phospholipid fatty acids reveal carbon cycling processes

ZHANG Yiming^1,2,,
HUANG Xianyu^1,2,3,,,
XIE Shucheng^1,2
1. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences(Wuhan), Wuhan 430078, Hubei
2. Hubei Key Laboratory of Critical Zone Evolution, China University of Geosciences(Wuhan), Wuhan 430074, Hubei
3. School of Geography and Information Engineering, China University of Geosciences(Wuhan), Wuhan 430078, Hubei


More Information

Corresponding author: HUANG Xianyu,E-mail:xyhuang@cug.edu.cn

MSC: P593;P534.63

--> Received Date: 20 November 2020
Revised Date: 18 February 2021
Publish Date: 30 July 2021


摘要
摘要:碳循环对于维持地球生命和调控气候变化具有重要意义。微生物是碳循环的关键驱动者之一，微生物群落结构和碳代谢活动的变化，能够影响温室气体的释放和碳储量，并进一步对气候变化产生反馈作用。在当前全球变化和人类活动加剧的背景下，需要进一步了解不同环境中的微生物群落组成和碳代谢活动的特征和响应。磷脂脂肪酸（PLFA）因其快速的周转速率和多样的分子结构，已经被广泛用于原位识别不同环境中活体微生物的生物量和群落结构。在此基础上，PLFA的单体碳同位素组成进一步指示了微生物利用哪些碳源，以及通过何种方式同化这些碳源，从而揭示微生物介导的碳流通和碳循环过程。本文综述了PLFA单体稳定碳同位素组成（δ¹³C）和放射性碳同位素组成（Δ¹⁴C）在示踪微生物碳循环过程中的应用。对比了农田、草地、森林、湿地以及海洋沉积物中活体微生物的碳源利用和碳同化途径的差异，这些差异可能与不同微生物对碳源的偏好以及不同环境中底物碳的可用性有关。对比了岩石、地下水、表层和深层土壤环境中微生物对化石碳源和年轻碳源的差异利用，化石碳是岩石和地下水环境中活体微生物碳源的重要组成部分，表明现代活体微生物可以直接利用部分老碳；相反，表层和深层土壤微生物倾向于利用现代碳源，这些碳源可能来自根系分泌物和从表面垂直输送的溶解有机碳。随着加速器质谱和单个化合物制备技术的进一步发展，预计PLFA单体放射性碳同位素将在微生物碳循环过程中发挥更重要的作用，值得在不同环境中进一步探索。
关键词: 碳循环/
微生物/
磷脂脂肪酸/
单体碳同位素/
碳源代谢/
老碳

Abstract:Earth's life-sustaining and climate change are highly associated with the carbon cycle process. Microorganisms are one of the most critical drivers of the carbon cycle. Changes in microbial community structure and carbon metabolic activities can affect greenhouse gas emissions and soil carbon storage, thus, have further feedback on climate change. In the context of future climate change and increased anthropogenic disturbance, it is essential to understand the microbial composition and carbon metabolic activities in different environments. Phospholipid fatty acids(PLFAs) have been widely used to identify in situ living microbial biomass and community structures in sediments such as soil. Specific living microbial communities can be linked to the unique molecular structure of PLFAs. On this basis, the compound-specific carbon isotope composition of PLFAs provides further information about microbial carbon sources, carbon assimilation pathways, and microbe-mediated carbon cycling processes. In this review, we summarized the applications of compound-specific stable carbon isotope compositions(δ¹³C) and radiocarbon isotope compositions(Δ¹⁴C) of PLFAs in tracing the microbial carbon cycle process. In farmland, grassland, forest, wetland and marine sediments, microbial communities show different strategies for substrate carbon utilization and assimilation pathway, which could be related to the availability of substrate carbon in these settings and microbial carbon utilization preferences. In rock and groundwater environments, fossil carbon represents an essential part of living microorganisms' carbon source, suggesting that modern living microorganisms can directly utilize some old and refractory carbon. On the contrary, soil living microorganisms tend to use modern carbon, which may come from root exudates and dissolved organic carbon transported vertically from the surface soil. With the development of accelerator mass spectrometry and preparative technology for individual PLFAs, the Δ¹⁴C of PLFAs is expected to play a more important role in exploring the microbial carbon cycle process in different environments.
Key words:carbon cycle/
microorganism/
phospholipid fatty acids/
compound-specific carbon isotope/
microbial carbon metabolism/
old carbon



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