Lilan Hao
Zhe Zhang
Liu Tian
Xiaowei Zhang
Jie Zhu
Zhuye Jie
Xin Tong
Liang Xiao
Tao Zhang
Xin Jin
Xun Xu
Huanming Yang
Jian Wang
Karsten Kristiansen
Huijue Jia
a BGI-Shenzhen, Shenzhen 518083, China;
b Shenzhen Key Laboratory of Human Commensal Microorganisms and Health Research, BGI-Shenzhen, Shenzhen 518083, China;
c Laboratory of Genomics and Molecular Biomedicine, Department of Biology, Universitetsparken 13, University of Copenhagen, DK-2100 Copenhagen, Denmark;
d Shenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome, BGI-Shenzhen, Shenzhen 518083, China;
e BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China;
f James D. Watson Institute of Genome Sciences, Hangzhou 310058, China
Funds: We are very grateful to colleagues at BGI-Shenzhen for sample collection, and discussions, and China National Genebank (CNGB) Shenzhen for DNA extraction, library construction, and sequencing.
Received Date: 2020-11-03
Accepted Date:2021-03-17
Rev Recd Date:2021-03-17
Publish Date:2021-08-20
Abstract
Abstract
The vaginal microbiota is less complex than the gut microbiota, and the colonization of Lactobacillus in the female vagina is considered to be critical for reproductive health. Oral probiotics have been suggested as promising means to modulate vaginal homeostasis in the general population. In this study, 60 Chinese women were followed for over a year before, during, and after treatment with the probiotics Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14. Shotgun metagenomic data of 1334 samples from multiple body sites did not support a colonization route of the probiotics from the oral cavity to the intestinal tract and then to the vagina. Our analyses enable the classification of the cervicovaginal microbiome into a stable state and a state of dysbiosis. The microbiome in the stable group steadily maintained a relatively high abundance of Lactobacilli over one year, which was not affected by probiotic intake, whereas in the dysbiosis group, the microbiota was more diverse and changed markedly over time. Data from a subset of the dysbiosis group suggests this subgroup possibly benefited from supplementation with the probiotics, indicating that probiotics supplementation can be prescribed for women in a subclinical microbiome setting of dysbiosis, providing opportunities for targeted and personalized microbiome reconstitution.Keywords: Oral probiotics,
Cervicovaginal microbiome,
Shotgun metagenomic data
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