Xiangning Ding
Shiyou Wang
Peiwen Ding
Zaoxu Xu
Jiankang Li
Mingyue Wang
Rong Xiang
Xiaoling Wang
Haoyu Wang
Qikai Feng
Jiaying Qiu
Feiyue Wang
Zhen Huang
Xingliang Zhang
Gen Tang
Shengping Tang
aBGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
bBGI-Shenzhen, Shenzhen 518083, China
cSchool of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China
dSouthern Center for Biomedical Research and Fujian Key Laboratory of Developmental and Neural Biology, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China
eShenzhen Children's Hospital, Shenzhen 518083, China
fDepartment of Pediatrics, the Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, China
gZunyi Medical University, Zunyi, Guizhou 563099, China
hChina Medical University, Shenyang, Liaoning 110122, China
More InformationCorresponding author: E-mail address: xingliang_zhang@163.com (Xingliang Zhang);E-mail address: genine56@126.com (Gen Tang);E-mail address: tangshengping56@126.com (Shengping Tang)
Received Date: 2020-08-06
Accepted Date:2021-02-01
Rev Recd Date:2021-01-26
Available Online: 2021-03-17 Publish Date:2021-02-20
Abstract
Abstract
Olfaction, the sense of smell, is a fundamental trait crucial to many species. The olfactory bulb (OB) plays pivotal roles in processing and transmitting odor information from the environment to the brain. The cellular heterogeneity of the mouse OB has been studied using single-cell RNA sequencing. However, the epigenetic landscape of the mOB remains mostly unexplored. Herein, we apply single-cell assay for transposase-accessible chromatin sequencing to profile the genome-wide chromatin accessibility of 9,549 single cells from the mOB. Based on single-cell epigenetic signatures, mOB cells are classified into 21 clusters corresponding to 11 cell types. We identify distinct sets of putative regulatory elements specific to each cell cluster from which putative target genes and enriched potential functions are inferred. In addition, the transcription factor motifs enriched in each cell cluster are determined to indicate the developmental fate of each cell lineage. Our study provides a valuable epigenetic data set for the mOB at single-cell resolution, and the results can enhance our understanding of regulatory circuits and the therapeutic capacity of the OB at the single-cell level.Keywords: scATAC-seq,
Mouse olfactory bulb,
Chromatin accessibility,
Transcription factor,
Endogenous retroviral elements,
Co-embedding analysis
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