Yifeng Liu
Yuli Qian
Dan Zhang
aKey Laboratory of Reproductive Genetics (Ministry of Education) and Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, China
bKey Laboratory of Women's Reproductive Health of Zhejiang Province, Hangzhou, Zhejiang 310006, China
More InformationCorresponding author: E-mail address: zhangdan@zju.edu.cn (Dan Zhang)
Publish Date:2020-12-25
Abstract
Abstract
Invasive genetic screening of pre-implantation embryos via biopsied trophectoderm (TE) cells has been in use for more than 20 years, while its benefits in selecting euploid embryos remain controversial. Recent advances in the ability to process embryonic cell-free DNA (cfDNA) from blastocoel fluid (BF) and spent culture media (SCM) of blastocysts in a manner similar to that of a biopsied TE sample provide a potential alternative holding great promise for obtaining cytogenetic information of the embryos without intrusive biopsy of traditional biopsy-based pre-implantation genetic testing (PGT). Several studies have reported even higher diagnostic accuracy in non-invasive PGT (ni-PGT) than conventional PGT. However, there are still several technical challenges to be overcome before ni-PGT can be accepted as a reliable genomic information source of embryo. In this review, we have summarized the emergence and current state of ni-PGT, and discussed our own perspectives on their limitations and future prospect. There is still a long way to go before truly wide clinical application of ni-PGT.Keywords: Pre-implantation genetic testing,
Spent culture media,
Blastocoel fluid,
Cell-free DNA,
Non-invasive PGT
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