Zhen Wang
Shumei Chai
Xiong Bai
Guohui Ding
Yuanyuan Li
Junyi Li
Qingyu Xiao
Benpeng Miao
Weili Lin
Jie Feng
Mingyue Huang
Cheng Gao
Bin Li
Wei Hu
Jiaojiao Lin
Zhiqiang Fu
Jianyun Xie
Yixue Li
aBio-Med Big Data Center, CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China
bShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200241, China
cShanghai Laboratory Animal Research Center, Shanghai 201203, China
dInstitute for Digital Health, International Human Phenome Institutes (Shanghai), Shanghai 200433, China
eShanghai Center for Bioinformation Technology, Shanghai Academy of Science and Technology, Shanghai 201203, China
fSchool of Computer Science and Technology, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong 518055, China
gShanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai JiaoTong University School of Medicine, Shanghai 200025, China
hState Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200438, China
iHangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 330106, China
More InformationCorresponding author: E-mail address: fuzhiqiang@shvri.ac.cn (Zhiqiang Fu);E-mail address: xiejianyun@slarc.org.cn (Jianyun Xie);E-mail address: yxli@sibs.ac.cn (Yixue Li)
Publish Date:2020-12-25
Abstract
Abstract
Microtus fortis is the only mammalian host that exhibits intrinsic resistance against Schistosoma japonicum infection. However, the underlying molecular mechanisms of this resistance are not yet known. Here, we perform the first de novo genome assembly of M.?fortis, comprehensive gene annotation analysis, and evolution analysis. Furthermore, we compare the recovery rate of schistosomes, pathological changes, and liver transcriptomes between M.?fortis and mice at different time points after infection. We observe that the time and type of immune response in M.?fortis are different from those in mice. M.?fortis activates immune and inflammatory responses on the 10th day post infection, such as leukocyte extravasation, antibody activation, Fc-gamma receptor-mediated phagocytosis, and the interferon signaling cascade, which play important roles in preventing the development of schistosomes. In contrast, an intense immune response occurrs in mice at the late stages of infection and could not eliminate schistosomes. Infected mice suffer severe pathological injury and continuous decreases in cell cycle, lipid metabolism, and other functions. Our findings offer new insights into the intrinsic resistance mechanism ofM.?fortis against schistosome infection. The genome sequence also provides the basis for future studies of other important traits in M.?fortis.Keywords: Genome assembly,
Schistosome,
Immune,
Transcriptome
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