Cell Reports
Abstract
Synaptonemal complex (SC) assembly and homologous recombination, the most critical events during prophase I, are the prerequisite for faithful meiotic chromosome segregation. However, the underlying regulatory mechanism remains largely unknown. Here, we reveal that a functional RING finger E3 ubiquitin ligase, DESYNAPSIS1 (DSNP1), plays significant roles in SC assembly and homologous recombination during rice meiosis. In thedsnp1mutant, homologous synapsis is discontinuous and aberrant SC-like polycomplexes occur independent of coaligned homologous chromosomes. Accompanying the decreased foci of HEI10, ZIP4, and MER3 on meiotic chromosomes, the number of crossovers (COs) decreases dramatically indsnp1meiocytes. Furthermore, the absence of central elements largely restores the localization of non-ZEP1 ZMM proteins and the number of COs in thedsnp1background. Collectively, DSNP1 stabilizes the canonical tripartite SC structure along paired homologous chromosomes and further promotes the formation of COs.
| 论文编号: | DOI:10.1016/j.celrep.2021.109941 |
| 论文题目: | The E3 Ubiquitin Ligase DESYNAPSIS1 Regulates Synapsis and Recombination in Rice Meiosis |
| 英文论文题目: | The E3 Ubiquitin Ligase DESYNAPSIS1 Regulates Synapsis and Recombination in Rice Meiosis |
| 第一作者: | Lijun Ren, Tingting Zhao, Yangzi Zhao, Guijie Du, Shuying Yang, Na Mu, Ding Tang, Yi Shen, Yafei Li, Zhukuan Cheng |
| 英文第一作者: | Lijun Ren, Tingting Zhao, Yangzi Zhao, Guijie Du, Shuying Yang, Na Mu, Ding Tang, Yi Shen, Yafei Li, Zhukuan Cheng |
| 联系作者: | |
| 英文联系作者: | |
| 外单位作者单位: | |
| 英文外单位作者单位: | |
| 发表年度: | 2021-11-03 |
| 卷: | |
| 期: | |
| 页码: | |
| 摘要: | Synaptonemal complex (SC) assembly and homologous recombination, the most critical events during prophase I, are the prerequisite for faithful meiotic chromosome segregation. However, the underlying regulatory mechanism remains largely unknown. Here, we reveal that a functional RING finger E3 ubiquitin ligase, DESYNAPSIS1 (DSNP1), plays significant roles in SC assembly and homologous recombination during rice meiosis. In thedsnp1mutant, homologous synapsis is discontinuous and aberrant SC-like polycomplexes occur independent of coaligned homologous chromosomes. Accompanying the decreased foci of HEI10, ZIP4, and MER3 on meiotic chromosomes, the number of crossovers (COs) decreases dramatically indsnp1meiocytes. Furthermore, the absence of central elements largely restores the localization of non-ZEP1 ZMM proteins and the number of COs in thedsnp1background. Collectively, DSNP1 stabilizes the canonical tripartite SC structure along paired homologous chromosomes and further promotes the formation of COs. |
| 英文摘要: | Synaptonemal complex (SC) assembly and homologous recombination, the most critical events during prophase I, are the prerequisite for faithful meiotic chromosome segregation. However, the underlying regulatory mechanism remains largely unknown. Here, we reveal that a functional RING finger E3 ubiquitin ligase, DESYNAPSIS1 (DSNP1), plays significant roles in SC assembly and homologous recombination during rice meiosis. In thedsnp1mutant, homologous synapsis is discontinuous and aberrant SC-like polycomplexes occur independent of coaligned homologous chromosomes. Accompanying the decreased foci of HEI10, ZIP4, and MER3 on meiotic chromosomes, the number of crossovers (COs) decreases dramatically indsnp1meiocytes. Furthermore, the absence of central elements largely restores the localization of non-ZEP1 ZMM proteins and the number of COs in thedsnp1background. Collectively, DSNP1 stabilizes the canonical tripartite SC structure along paired homologous chromosomes and further promotes the formation of COs. |
| 刊物名称: | Cell Reports |
| 英文刊物名称: | Cell Reports |
| 论文全文: | |
| 英文论文全文: | |
| 全文链接: | |
| 其它备注: | Lijun Ren, Tingting Zhao, Yangzi Zhao, Guijie Du, Shuying Yang, Na Mu, Ding Tang, Yi Shen, Yafei Li, Zhukuan Cheng. The E3 Ubiquitin Ligase DESYNAPSIS1 Regulates Synapsis and Recombination in Rice Meiosis. Cell Reports. DOI:10.1016/j.celrep.2021.109941 |
| 英文其它备注: | |
| 学科: | |
| 英文学科: | |
| 影响因子: | |
| 第一作者所在部门: | |
| 英文第一作者所在部门: | |
| 论文出处: | |
| 英文论文出处: | |
| 论文类别: | |
| 英文论文类别: | |
| 参与作者: | |
| 英文参与作者: |
