Molecular & Cellular Proteomics
Abstract
Light is essential for photosynthetic organisms and is involved in the regulation of protein synthesis and degradation. The significance of light-regulated protein degradation is exemplified by the well-established light-induced degradation and repair of the photosystem II reaction center D1 protein in higher plants and cyanobacteria. However, systematic studies of light-regulated protein degradation events in photosynthetic organisms are lacking. Thus, we conducted a large-scale survey of protein degradation under light or dark conditions in the model cyanobacterium Synechocystis sp. PCC 6803 using the isobaric labeling-based quantitative proteomics technique. The results revealed that 79 proteins showed light-regulated degradation, including proteins involved in photosystem II structure or function, quinone binding and NADH dehydrogenase. Among these, 25 proteins were strongly dependent on light for degradation. Moreover, the light-dependent degradation of several proteins was sensitive to photosynthetic electron transport inhibitors (DCMU and DBMIB), suggesting that they are influenced by the redox state of the plastoquinone (PQ) pool. Together, our study comprehensively cataloged light-regulated protein degradation events, and the results serve as an important resource for future studies aimed at understanding light-regulated processes and protein quality control mechanisms in cyanobacteria.
| 论文编号: | DOI:10.1016/j.mcpro.2021.100162 |
| 论文题目: | A Systematic Survey of the Light/Dark-Dependent Protein Degradation Events in a Model Cyanobacterium |
| 英文论文题目: | A Systematic Survey of the Light/Dark-Dependent Protein Degradation Events in a Model Cyanobacterium |
| 第一作者: | Weiyang Chen, Limin Zheng, Jinghui Dong, Haitao Ge, Xiahe Huang, Gaojie Wang, Chengcheng Huang, Yan Wang, Dandan Lu, Wu Xu, and Yingchun Wang |
| 英文第一作者: | Weiyang Chen, Limin Zheng, Jinghui Dong, Haitao Ge, Xiahe Huang, Gaojie Wang, Chengcheng Huang, Yan Wang, Dandan Lu, Wu Xu, and Yingchun Wang |
| 联系作者: | |
| 英文联系作者: | |
| 外单位作者单位: | |
| 英文外单位作者单位: | |
| 发表年度: | 2021-10-20 |
| 卷: | |
| 期: | |
| 页码: | |
| 摘要: | Light is essential for photosynthetic organisms and is involved in the regulation of protein synthesis and degradation. The significance of light-regulated protein degradation is exemplified by the well-established light-induced degradation and repair of the photosystem II reaction center D1 protein in higher plants and cyanobacteria. However, systematic studies of light-regulated protein degradation events in photosynthetic organisms are lacking. Thus, we conducted a large-scale survey of protein degradation under light or dark conditions in the model cyanobacterium Synechocystis sp. PCC 6803 using the isobaric labeling-based quantitative proteomics technique. The results revealed that 79 proteins showed light-regulated degradation, including proteins involved in photosystem II structure or function, quinone binding and NADH dehydrogenase. Among these, 25 proteins were strongly dependent on light for degradation. Moreover, the light-dependent degradation of several proteins was sensitive to photosynthetic electron transport inhibitors (DCMU and DBMIB), suggesting that they are influenced by the redox state of the plastoquinone (PQ) pool. Together, our study comprehensively cataloged light-regulated protein degradation events, and the results serve as an important resource for future studies aimed at understanding light-regulated processes and protein quality control mechanisms in cyanobacteria. |
| 英文摘要: | Light is essential for photosynthetic organisms and is involved in the regulation of protein synthesis and degradation. The significance of light-regulated protein degradation is exemplified by the well-established light-induced degradation and repair of the photosystem II reaction center D1 protein in higher plants and cyanobacteria. However, systematic studies of light-regulated protein degradation events in photosynthetic organisms are lacking. Thus, we conducted a large-scale survey of protein degradation under light or dark conditions in the model cyanobacterium Synechocystis sp. PCC 6803 using the isobaric labeling-based quantitative proteomics technique. The results revealed that 79 proteins showed light-regulated degradation, including proteins involved in photosystem II structure or function, quinone binding and NADH dehydrogenase. Among these, 25 proteins were strongly dependent on light for degradation. Moreover, the light-dependent degradation of several proteins was sensitive to photosynthetic electron transport inhibitors (DCMU and DBMIB), suggesting that they are influenced by the redox state of the plastoquinone (PQ) pool. Together, our study comprehensively cataloged light-regulated protein degradation events, and the results serve as an important resource for future studies aimed at understanding light-regulated processes and protein quality control mechanisms in cyanobacteria. |
| 刊物名称: | Molecular & Cellular Proteomics |
| 英文刊物名称: | Molecular & Cellular Proteomics |
| 论文全文: | |
| 英文论文全文: | |
| 全文链接: | |
| 其它备注: | Weiyang Chen, Limin Zheng, Jinghui Dong, Haitao Ge, Xiahe Huang, Gaojie Wang, Chengcheng Huang, Yan Wang, Dandan Lu, Wu Xu, and Yingchun Wang. A Systematic Survey of the Light/Dark-Dependent Protein Degradation Events in a Model Cyanobacterium. Molecular & Cellular Proteomics. DOI:10.1016/j.mcpro.2021.100162 |
| 英文其它备注: | |
| 学科: | |
| 英文学科: | |
| 影响因子: | |
| 第一作者所在部门: | |
| 英文第一作者所在部门: | |
| 论文出处: | |
| 英文论文出处: | |
| 论文类别: | |
| 英文论文类别: | |
| 参与作者: | |
| 英文参与作者: |
