EMBO Reports
Abstract
During central nervous system development, neurogenesis and gliogenesis occur in an orderly manner to create precise neural circuitry. However, no systematic dataset of neural lineage development that covers both neurogenesis and gliogenesis for the human spinal cord is available. We here perform single-cell RNA sequencing of human spinal cord cells during embryonic and fetal stages that cover neuron generation as well as astrocytes and oligodendrocyte differentiation. We also map the timeline of sensory neurogenesis and gliogenesis in the spinal cord. We further identify a group of EGFR-expressing transitional glial cells with radial morphology at the onset of gliogenesis, which progressively acquires differentiated glial cell characteristics. These EGFR-expressing transitional glial cells exhibited a unique position-specific feature during spinal cord development. Cell crosstalk analysis using CellPhoneDB indicated that EGFR glial cells can persistently interact with other neural cells during development through Delta-Notch and EGFR signaling. Together, our results reveal stage-specific profiles and dynamics of neural cells during human spinal cord development.
| 论文编号: | DOI:10.15252/embr.202152728 |
| 论文题目: | Single-Cell Analysis Reveals Dynamic Changes of Neural Cells in Developing Human Spinal Cord |
| 英文论文题目: | Single-Cell Analysis Reveals Dynamic Changes of Neural Cells in Developing Human Spinal Cord |
| 第一作者: | Qi Zhang, Xianming Wu, Yongheng Fan, Peipei Jiang, Yannan Zhao, Yaming Yang, Sufang Han, Bai Xu, Bing Chen, Jin Han, Minghan Sun, Guangfeng Zhao, Zhifeng Xiao, Yali Hu, Jianwu Dai |
| 英文第一作者: | Qi Zhang, Xianming Wu, Yongheng Fan, Peipei Jiang, Yannan Zhao, Yaming Yang, Sufang Han, Bai Xu, Bing Chen, Jin Han, Minghan Sun, Guangfeng Zhao, Zhifeng Xiao, Yali Hu, Jianwu Dai |
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| 发表年度: | 2021-10-08 |
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| 摘要: | During central nervous system development, neurogenesis and gliogenesis occur in an orderly manner to create precise neural circuitry. However, no systematic dataset of neural lineage development that covers both neurogenesis and gliogenesis for the human spinal cord is available. We here perform single-cell RNA sequencing of human spinal cord cells during embryonic and fetal stages that cover neuron generation as well as astrocytes and oligodendrocyte differentiation. We also map the timeline of sensory neurogenesis and gliogenesis in the spinal cord. We further identify a group of EGFR-expressing transitional glial cells with radial morphology at the onset of gliogenesis, which progressively acquires differentiated glial cell characteristics. These EGFR-expressing transitional glial cells exhibited a unique position-specific feature during spinal cord development. Cell crosstalk analysis using CellPhoneDB indicated that EGFR glial cells can persistently interact with other neural cells during development through Delta-Notch and EGFR signaling. Together, our results reveal stage-specific profiles and dynamics of neural cells during human spinal cord development. |
| 英文摘要: | During central nervous system development, neurogenesis and gliogenesis occur in an orderly manner to create precise neural circuitry. However, no systematic dataset of neural lineage development that covers both neurogenesis and gliogenesis for the human spinal cord is available. We here perform single-cell RNA sequencing of human spinal cord cells during embryonic and fetal stages that cover neuron generation as well as astrocytes and oligodendrocyte differentiation. We also map the timeline of sensory neurogenesis and gliogenesis in the spinal cord. We further identify a group of EGFR-expressing transitional glial cells with radial morphology at the onset of gliogenesis, which progressively acquires differentiated glial cell characteristics. These EGFR-expressing transitional glial cells exhibited a unique position-specific feature during spinal cord development. Cell crosstalk analysis using CellPhoneDB indicated that EGFR glial cells can persistently interact with other neural cells during development through Delta-Notch and EGFR signaling. Together, our results reveal stage-specific profiles and dynamics of neural cells during human spinal cord development. |
| 刊物名称: | EMBO Reports |
| 英文刊物名称: | EMBO Reports |
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| 其它备注: | Qi Zhang, Xianming Wu, Yongheng Fan, Peipei Jiang, Yannan Zhao, Yaming Yang, Sufang Han, Bai Xu, Bing Chen, Jin Han, Minghan Sun, Guangfeng Zhao, Zhifeng Xiao, Yali Hu, Jianwu Dai. Single-Cell Analysis Reveals Dynamic Changes of Neural Cells in Developing Human Spinal Cord. EMBO Reports. DOI:10.15252/embr.202152728 |
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