SCIENCE CHINA Life Sciences
Abstract
Timely programmed cell death (PCD) of the tapetum supplying nutrients to microspores is a prerequisite for normal pollen development. Here we identified a unique mutant of rice (Oryza sativa L.), pollen sterility (post), which showed aborted pollens accompanied with extra-large husks. Due to failure of timely PCD of tapetal cells, post exhibited abnormal pollen wall patterning and defective pollen grains. By map-based cloning, we identified a causal gene, POST, encoding a novel protein which is ubiquitously localized in cells. RNA in situ hybridization showed that POST is highly detected in the tapetum and microspores at stages 8 and 9. Transcriptome analysis indicated that POST could function as an important regulator of the metabolic process involved in tapetal PCD. Compared with wild-type rice, post mutant has an increased cell number resulting from elevated expression of cell cycle associated genes in grain husks. Overexpression of POST inhibits grain size in wild type, while appropriate expression of POST in post mutant can recover the seed fertility but has little effect on the large grains, illustrating that fine-tuning of POST expression could be a potential strategy for rice yield improvement. The connection between cell division and cell death conferred by POST provides novel insights into understanding of the tapetal PCD process.
| 论文编号: | DOI:10.1007/s11427-021-2011-2 |
| 论文题目: | POLLEN STERILITY, a Novel Suppressor of Cell Division, is Required for Timely Tapetal Programmed Cell Death in Rice |
| 英文论文题目: | POLLEN STERILITY, a Novel Suppressor of Cell Division, is Required for Timely Tapetal Programmed Cell Death in Rice |
| 第一作者: | Che Ronghui, Hu Bin, Wang Wei, Xiao Yunhua, Liu Dapu, Yin Wenchao, Tong Hongning and Chu Chengcai |
| 英文第一作者: | Che Ronghui, Hu Bin, Wang Wei, Xiao Yunhua, Liu Dapu, Yin Wenchao, Tong Hongning and Chu Chengcai |
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| 发表年度: | 2021-09-23 |
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| 摘要: | Timely programmed cell death (PCD) of the tapetum supplying nutrients to microspores is a prerequisite for normal pollen development. Here we identified a unique mutant of rice (Oryza sativa L.), pollen sterility (post), which showed aborted pollens accompanied with extra-large husks. Due to failure of timely PCD of tapetal cells, post exhibited abnormal pollen wall patterning and defective pollen grains. By map-based cloning, we identified a causal gene, POST, encoding a novel protein which is ubiquitously localized in cells. RNA in situ hybridization showed that POST is highly detected in the tapetum and microspores at stages 8 and 9. Transcriptome analysis indicated that POST could function as an important regulator of the metabolic process involved in tapetal PCD. Compared with wild-type rice, post mutant has an increased cell number resulting from elevated expression of cell cycle associated genes in grain husks. Overexpression of POST inhibits grain size in wild type, while appropriate expression of POST in post mutant can recover the seed fertility but has little effect on the large grains, illustrating that fine-tuning of POST expression could be a potential strategy for rice yield improvement. The connection between cell division and cell death conferred by POST provides novel insights into understanding of the tapetal PCD process. |
| 英文摘要: | Timely programmed cell death (PCD) of the tapetum supplying nutrients to microspores is a prerequisite for normal pollen development. Here we identified a unique mutant of rice (Oryza sativa L.), pollen sterility (post), which showed aborted pollens accompanied with extra-large husks. Due to failure of timely PCD of tapetal cells, post exhibited abnormal pollen wall patterning and defective pollen grains. By map-based cloning, we identified a causal gene, POST, encoding a novel protein which is ubiquitously localized in cells. RNA in situ hybridization showed that POST is highly detected in the tapetum and microspores at stages 8 and 9. Transcriptome analysis indicated that POST could function as an important regulator of the metabolic process involved in tapetal PCD. Compared with wild-type rice, post mutant has an increased cell number resulting from elevated expression of cell cycle associated genes in grain husks. Overexpression of POST inhibits grain size in wild type, while appropriate expression of POST in post mutant can recover the seed fertility but has little effect on the large grains, illustrating that fine-tuning of POST expression could be a potential strategy for rice yield improvement. The connection between cell division and cell death conferred by POST provides novel insights into understanding of the tapetal PCD process. |
| 刊物名称: | SCIENCE CHINA Life Sciences |
| 英文刊物名称: | SCIENCE CHINA Life Sciences |
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| 其它备注: | Che Ronghui, Hu Bin, Wang Wei, Xiao Yunhua, Liu Dapu, Yin Wenchao, Tong Hongning and Chu Chengcai. POLLEN STERILITY, a Novel Suppressor of Cell Division, is Required for Timely Tapetal Programmed Cell Death in Rice. SCIENCE CHINA Life Sciences. DOI:10.1007/s11427-021-2011-2 |
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