Plant Biotechnology Journal
Abstract
Soybean is an important crop worldwide, but its production is severely affected by salt stress. Understanding the regulatory mechanism of salt response is crucial for improving the salt tolerance of soybean. Here, we reveal a role for nuclear factor Y subunit GmNFYA in salt tolerance of soybean likely through regulation of histone acetylation.GmNFYAis induced by salt stress. Overexpression ofGmNFYAsignificantly enhances salt tolerance in stable transgenic soybean plants by inducing salt-responsive genes. Analysis in soybean plants with transgenic hairy roots also supports the conclusion. GmNFYA interacts with GmFVE, which functions with putative histone deacetylase GmHDA13 in a complex for transcriptional repression possibly by reducing H3K9 acetylation at target loci. Under salt stress, GmNFYA likely accumulates and competes with GmHDA13 for interaction with GmFVE, leading to derepression and maintenance of histone acetylation for activation of salt-responsive genes, and finally conferring salt tolerance in soybean plants. In addition, a haplotype IGmNFYApromoter is identified with highest self-activated promoter activity and may be selected during future breeding for salt tolerant cultivars. Our study uncovers the epigenetic regulatory mechanism ofGmNFYAin salt-stress response and all the factors/elements identified may be potential targets for genetic manipulation of salt tolerance in soybean and other crops.
| 论文编号: | DOI:10.1111/pbi.13668 |
| 论文题目: | Nuclear Factor Y Subunit GmNFYA Competes with GmHDA13 for Interaction with GmFVE to Positively Regulate Salt Tolerance in Soybean |
| 英文论文题目: | Nuclear Factor Y Subunit GmNFYA Competes with GmHDA13 for Interaction with GmFVE to Positively Regulate Salt Tolerance in Soybean |
| 第一作者: | Long Lu, Wei Wei, Jianjun Tao, Xiang Lu, Xiaohua Bian, Yang Hu, Tong Cheng, Cuicui Yin, Wanke Zhang, Shouyi Chen, Jinsong Zhang |
| 英文第一作者: | Long Lu, Wei Wei, Jianjun Tao, Xiang Lu, Xiaohua Bian, Yang Hu, Tong Cheng, Cuicui Yin, Wanke Zhang, Shouyi Chen, Jinsong Zhang |
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| 发表年度: | 2021-07-19 |
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| 摘要: | Soybean is an important crop worldwide, but its production is severely affected by salt stress. Understanding the regulatory mechanism of salt response is crucial for improving the salt tolerance of soybean. Here, we reveal a role for nuclear factor Y subunit GmNFYA in salt tolerance of soybean likely through regulation of histone acetylation.GmNFYAis induced by salt stress. Overexpression ofGmNFYAsignificantly enhances salt tolerance in stable transgenic soybean plants by inducing salt-responsive genes. Analysis in soybean plants with transgenic hairy roots also supports the conclusion. GmNFYA interacts with GmFVE, which functions with putative histone deacetylase GmHDA13 in a complex for transcriptional repression possibly by reducing H3K9 acetylation at target loci. Under salt stress, GmNFYA likely accumulates and competes with GmHDA13 for interaction with GmFVE, leading to derepression and maintenance of histone acetylation for activation of salt-responsive genes, and finally conferring salt tolerance in soybean plants. In addition, a haplotype IGmNFYApromoter is identified with highest self-activated promoter activity and may be selected during future breeding for salt tolerant cultivars. Our study uncovers the epigenetic regulatory mechanism ofGmNFYAin salt-stress response and all the factors/elements identified may be potential targets for genetic manipulation of salt tolerance in soybean and other crops. |
| 英文摘要: | Soybean is an important crop worldwide, but its production is severely affected by salt stress. Understanding the regulatory mechanism of salt response is crucial for improving the salt tolerance of soybean. Here, we reveal a role for nuclear factor Y subunit GmNFYA in salt tolerance of soybean likely through regulation of histone acetylation.GmNFYAis induced by salt stress. Overexpression ofGmNFYAsignificantly enhances salt tolerance in stable transgenic soybean plants by inducing salt-responsive genes. Analysis in soybean plants with transgenic hairy roots also supports the conclusion. GmNFYA interacts with GmFVE, which functions with putative histone deacetylase GmHDA13 in a complex for transcriptional repression possibly by reducing H3K9 acetylation at target loci. Under salt stress, GmNFYA likely accumulates and competes with GmHDA13 for interaction with GmFVE, leading to derepression and maintenance of histone acetylation for activation of salt-responsive genes, and finally conferring salt tolerance in soybean plants. In addition, a haplotype IGmNFYApromoter is identified with highest self-activated promoter activity and may be selected during future breeding for salt tolerant cultivars. Our study uncovers the epigenetic regulatory mechanism ofGmNFYAin salt-stress response and all the factors/elements identified may be potential targets for genetic manipulation of salt tolerance in soybean and other crops. |
| 刊物名称: | Plant Biotechnology Journal |
| 英文刊物名称: | Plant Biotechnology Journal |
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| 其它备注: | Long Lu, Wei Wei, Jianjun Tao, Xiang Lu, Xiaohua Bian, Yang Hu, Tong Cheng, Cuicui Yin, Wanke Zhang, Shouyi Chen, Jinsong Zhang. Nuclear Factor Y Subunit GmNFYA Competes with GmHDA13 for Interaction with GmFVE to Positively Regulate Salt Tolerance in Soybean. Plant Biotechnology Journal. DOI:10.1111/pbi.13668 |
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