New Phytologist
Abstract
Hybrid necrosis, caused by complementary genesNe1andNe2, is a serious barrier for combining desirable traits from different genotypes of wheat, affecting the full utilization of heterosis. Until now, bothNe1andNe2are still not isolated although they were documented decades ago.
We report here the map-based cloning and functional characterization ofNe2, encoding a coiled coil-nucleotide-binding site-leucine-rich repeat (CC-NBS-LRR) protein. Homozygous frameshift mutations generated by CRISPR/Cas9 approach confirmed theNe2-inducing hybrid necrosis in wheat. Up-regulated expression ofNe2induced byNe1and excess hydrogen peroxide accumulation are associated with the necrosis formation.
Genetic analyses of aNe2allele (Ne2m) and leaf rust resistance geneLrLC10/Lr13revealed that they might be the same gene. Further, we demonstrated that the frequency ofNe2allele was much lower in landraces (2.00%) than that in modern cultivars (13.62%), suggesting thatNe2allele has been partially applied in wheat genetic improvement.
Our findings open opportunities of thoroughly investigating the molecular mechanism of hybrid necrosis, selectingLr13and simultaneously avoiding hybrid necrosis in wheat breeding through marker-assisted selection.
| 论文编号: | DOI:10.1111/nph.17575 |
| 论文题目: | Ne2, A Typical CC-NBS-LRR-Type Gene, is Responsible for Hybrid Necrosis in Wheat |
| 英文论文题目: | Ne2, A Typical CC-NBS-LRR-Type Gene, is Responsible for Hybrid Necrosis in Wheat |
| 第一作者: | Yaoqi Si, Shusong Zheng, Jianqing Niu, Shuiquan Tian, Mengjun Gu, Qiao Lu, Yilin He, Juan Zhang, Xiaoli Shi, Yiwen Li, Hong-Qing Ling |
| 英文第一作者: | Yaoqi Si, Shusong Zheng, Jianqing Niu, Shuiquan Tian, Mengjun Gu, Qiao Lu, Yilin He, Juan Zhang, Xiaoli Shi, Yiwen Li, Hong-Qing Ling |
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| 发表年度: | 2021-06-25 |
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| 摘要: | Hybrid necrosis, caused by complementary genesNe1andNe2, is a serious barrier for combining desirable traits from different genotypes of wheat, affecting the full utilization of heterosis. Until now, bothNe1andNe2are still not isolated although they were documented decades ago. We report here the map-based cloning and functional characterization ofNe2, encoding a coiled coil-nucleotide-binding site-leucine-rich repeat (CC-NBS-LRR) protein. Homozygous frameshift mutations generated by CRISPR/Cas9 approach confirmed theNe2-inducing hybrid necrosis in wheat. Up-regulated expression ofNe2induced byNe1and excess hydrogen peroxide accumulation are associated with the necrosis formation. Genetic analyses of aNe2allele (Ne2m) and leaf rust resistance geneLrLC10/Lr13revealed that they might be the same gene. Further, we demonstrated that the frequency ofNe2allele was much lower in landraces (2.00%) than that in modern cultivars (13.62%), suggesting thatNe2allele has been partially applied in wheat genetic improvement. Our findings open opportunities of thoroughly investigating the molecular mechanism of hybrid necrosis, selectingLr13and simultaneously avoiding hybrid necrosis in wheat breeding through marker-assisted selection. |
| 英文摘要: | Hybrid necrosis, caused by complementary genesNe1andNe2, is a serious barrier for combining desirable traits from different genotypes of wheat, affecting the full utilization of heterosis. Until now, bothNe1andNe2are still not isolated although they were documented decades ago. We report here the map-based cloning and functional characterization ofNe2, encoding a coiled coil-nucleotide-binding site-leucine-rich repeat (CC-NBS-LRR) protein. Homozygous frameshift mutations generated by CRISPR/Cas9 approach confirmed theNe2-inducing hybrid necrosis in wheat. Up-regulated expression ofNe2induced byNe1and excess hydrogen peroxide accumulation are associated with the necrosis formation. Genetic analyses of aNe2allele (Ne2m) and leaf rust resistance geneLrLC10/Lr13revealed that they might be the same gene. Further, we demonstrated that the frequency ofNe2allele was much lower in landraces (2.00%) than that in modern cultivars (13.62%), suggesting thatNe2allele has been partially applied in wheat genetic improvement. Our findings open opportunities of thoroughly investigating the molecular mechanism of hybrid necrosis, selectingLr13and simultaneously avoiding hybrid necrosis in wheat breeding through marker-assisted selection. |
| 刊物名称: | New Phytologist |
| 英文刊物名称: | New Phytologist |
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| 其它备注: | Yaoqi Si, Shusong Zheng, Jianqing Niu, Shuiquan Tian, Mengjun Gu, Qiao Lu, Yilin He, Juan Zhang, Xiaoli Shi, Yiwen Li, Hong-Qing Ling. Ne2, A Typical CC-NBS-LRR-Type Gene, is Responsible for Hybrid Necrosis in Wheat. New Phytologist. DOI:10.1111/nph.17575 |
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