Plant Biotechnology Journal
Abstract
Due to climate change, drought has become a severe abiotic stress that affects the global production of all crops. Elucidation of the complex physiological mechanisms underlying drought tolerance in crops will support the cultivation of new drought-tolerant crop varieties. Here, two drought-tolerant lines, RIL70 and RIL73, and two drought-sensitive lines, RIL44 and RIL93, from recombinant inbred lines (RIL) generated from maize drought-tolerant line PH4CV and drought-sensitive line F9721, were selected for a comparative RNA-seq study. Through transcriptome analyses, we found that gene expression differences existed between drought-tolerant and -sensitive lines, but also differences between the drought-tolerant lines, RIL70 and RIL73.ZmbHLH124in RIL73, named asZmbHLH124T-ORGwhich origins from PH4CV and encodes a bHLH type transcription factor, was specifically up-regulated during drought stress. In addition we identified a substitution inZmbHLH124that produced an early stop codon in sensitive lines (ZmbHLH124S-ORG). Overexpression ofZmbHLH124T-ORG, but notZmbHLH124S-ORG, in maize and rice enhanced plant drought tolerance and up-regulated the expression of drought-responsive genes. Moreover, we found that ZmbHLH124T-ORGcould directly bind thecis-acting elements inZmDREB2Apromoter to enhance its expression. Taken together, this work identified a valuable genetic locus and provided a new strategy for breeding drought-tolerant crops.
| 论文编号: | DOI:10.1111/pbi.13637 |
| 论文题目: | ZmbHLH124 Identified in Maize Recombinant Inbred Lines Contributes to Drought Tolerance in Crops |
| 英文论文题目: | ZmbHLH124 Identified in Maize Recombinant Inbred Lines Contributes to Drought Tolerance in Crops |
| 第一作者: | Shaowei Wei, Ran Xia, Chengxuan Chen, Xiaoling Shang, Fengyong Ge, Huimin Wei, Huabang Chen, Yaorong Wu, Qi Xie |
| 英文第一作者: | Shaowei Wei, Ran Xia, Chengxuan Chen, Xiaoling Shang, Fengyong Ge, Huimin Wei, Huabang Chen, Yaorong Wu, Qi Xie |
| 联系作者: | |
| 英文联系作者: | |
| 外单位作者单位: | |
| 英文外单位作者单位: | |
| 发表年度: | 2021-05-27 |
| 卷: | |
| 期: | |
| 页码: | |
| 摘要: | Due to climate change, drought has become a severe abiotic stress that affects the global production of all crops. Elucidation of the complex physiological mechanisms underlying drought tolerance in crops will support the cultivation of new drought-tolerant crop varieties. Here, two drought-tolerant lines, RIL70 and RIL73, and two drought-sensitive lines, RIL44 and RIL93, from recombinant inbred lines (RIL) generated from maize drought-tolerant line PH4CV and drought-sensitive line F9721, were selected for a comparative RNA-seq study. Through transcriptome analyses, we found that gene expression differences existed between drought-tolerant and -sensitive lines, but also differences between the drought-tolerant lines, RIL70 and RIL73.ZmbHLH124in RIL73, named asZmbHLH124T-ORGwhich origins from PH4CV and encodes a bHLH type transcription factor, was specifically up-regulated during drought stress. In addition we identified a substitution inZmbHLH124that produced an early stop codon in sensitive lines (ZmbHLH124S-ORG). Overexpression ofZmbHLH124T-ORG, but notZmbHLH124S-ORG, in maize and rice enhanced plant drought tolerance and up-regulated the expression of drought-responsive genes. Moreover, we found that ZmbHLH124T-ORGcould directly bind thecis-acting elements inZmDREB2Apromoter to enhance its expression. Taken together, this work identified a valuable genetic locus and provided a new strategy for breeding drought-tolerant crops. |
| 英文摘要: | Due to climate change, drought has become a severe abiotic stress that affects the global production of all crops. Elucidation of the complex physiological mechanisms underlying drought tolerance in crops will support the cultivation of new drought-tolerant crop varieties. Here, two drought-tolerant lines, RIL70 and RIL73, and two drought-sensitive lines, RIL44 and RIL93, from recombinant inbred lines (RIL) generated from maize drought-tolerant line PH4CV and drought-sensitive line F9721, were selected for a comparative RNA-seq study. Through transcriptome analyses, we found that gene expression differences existed between drought-tolerant and -sensitive lines, but also differences between the drought-tolerant lines, RIL70 and RIL73.ZmbHLH124in RIL73, named asZmbHLH124T-ORGwhich origins from PH4CV and encodes a bHLH type transcription factor, was specifically up-regulated during drought stress. In addition we identified a substitution inZmbHLH124that produced an early stop codon in sensitive lines (ZmbHLH124S-ORG). Overexpression ofZmbHLH124T-ORG, but notZmbHLH124S-ORG, in maize and rice enhanced plant drought tolerance and up-regulated the expression of drought-responsive genes. Moreover, we found that ZmbHLH124T-ORGcould directly bind thecis-acting elements inZmDREB2Apromoter to enhance its expression. Taken together, this work identified a valuable genetic locus and provided a new strategy for breeding drought-tolerant crops. |
| 刊物名称: | Plant Biotechnology Journal |
| 英文刊物名称: | Plant Biotechnology Journal |
| 论文全文: | |
| 英文论文全文: | |
| 全文链接: | |
| 其它备注: | Shaowei Wei, Ran Xia, Chengxuan Chen, Xiaoling Shang, Fengyong Ge, Huimin Wei, Huabang Chen, Yaorong Wu, Qi Xie. ZmbHLH124 Identified in Maize Recombinant Inbred Lines Contributes to Drought Tolerance in Crops. Plant Biotechnology Journal. DOI:10.1111/pbi.13637 |
| 英文其它备注: | |
| 学科: | |
| 英文学科: | |
| 影响因子: | |
| 第一作者所在部门: | |
| 英文第一作者所在部门: | |
| 论文出处: | |
| 英文论文出处: | |
| 论文类别: | |
| 英文论文类别: | |
| 参与作者: | |
| 英文参与作者: |
