The Plant Cell
Abstract
Endoplasmic reticulum-associated degradation (ERAD) is known to regulate plant responses to diverse stresses, yet its underlying molecular mechanisms and links to various stress signaling pathways are poorly understood. Here, we show that the ERAD component ubiquitin-conjugating enzyme UBC32 positively regulates drought tolerance in Arabidopsis thaliana by targeting the aquaporins PIP2;1 and PIP2;2 for degradation. Furthermore, we demonstrate that the RING-type ligase Rma1 acts together with UBC32 and that the E2 activity of UBC32 is essential for the ubiquitination of Rma1. This complex ubiquitinates a phosphorylated form of PIP2;1 at Lys276 to promote its degradation, thereby enhancing plant drought tolerance. Extending these molecular insights into crops, we show that overexpression of Arabidopsis UBC32 also improves drought tolerance in rice (Oryza sativa). Thus, beyond uncovering the molecular basis of an ERAD-regulated stress response, our study suggests multiple potential strategies for engineering crops with improved drought tolerance.
| 论文编号: | DOI:10.1093/plcell/koab141 |
| 论文题目: | ERAD-Related E2 and E3 Enzymes Modulate the Drought Response by Regulating the Stability of PIP2 Aquaporins |
| 英文论文题目: | ERAD-Related E2 and E3 Enzymes Modulate the Drought Response by Regulating the Stability of PIP2 Aquaporins |
| 第一作者: | Qian Chen, Ruijun Liu, Yaorong Wu, Shaowei Wei, Qian Wang, Yunna Zheng, Ran Xia, Xiaoling Shang, Feifei Yu, Xiaoyuan Yang, Lijing Liu, Xiahe Huang, Yingchun Wang and Qi Xie |
| 英文第一作者: | Qian Chen, Ruijun Liu, Yaorong Wu, Shaowei Wei, Qian Wang, Yunna Zheng, Ran Xia, Xiaoling Shang, Feifei Yu, Xiaoyuan Yang, Lijing Liu, Xiahe Huang, Yingchun Wang and Qi Xie |
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| 发表年度: | 2021-05-21 |
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| 摘要: | Endoplasmic reticulum-associated degradation (ERAD) is known to regulate plant responses to diverse stresses, yet its underlying molecular mechanisms and links to various stress signaling pathways are poorly understood. Here, we show that the ERAD component ubiquitin-conjugating enzyme UBC32 positively regulates drought tolerance in Arabidopsis thaliana by targeting the aquaporins PIP2;1 and PIP2;2 for degradation. Furthermore, we demonstrate that the RING-type ligase Rma1 acts together with UBC32 and that the E2 activity of UBC32 is essential for the ubiquitination of Rma1. This complex ubiquitinates a phosphorylated form of PIP2;1 at Lys276 to promote its degradation, thereby enhancing plant drought tolerance. Extending these molecular insights into crops, we show that overexpression of Arabidopsis UBC32 also improves drought tolerance in rice (Oryza sativa). Thus, beyond uncovering the molecular basis of an ERAD-regulated stress response, our study suggests multiple potential strategies for engineering crops with improved drought tolerance. |
| 英文摘要: | Endoplasmic reticulum-associated degradation (ERAD) is known to regulate plant responses to diverse stresses, yet its underlying molecular mechanisms and links to various stress signaling pathways are poorly understood. Here, we show that the ERAD component ubiquitin-conjugating enzyme UBC32 positively regulates drought tolerance in Arabidopsis thaliana by targeting the aquaporins PIP2;1 and PIP2;2 for degradation. Furthermore, we demonstrate that the RING-type ligase Rma1 acts together with UBC32 and that the E2 activity of UBC32 is essential for the ubiquitination of Rma1. This complex ubiquitinates a phosphorylated form of PIP2;1 at Lys276 to promote its degradation, thereby enhancing plant drought tolerance. Extending these molecular insights into crops, we show that overexpression of Arabidopsis UBC32 also improves drought tolerance in rice (Oryza sativa). Thus, beyond uncovering the molecular basis of an ERAD-regulated stress response, our study suggests multiple potential strategies for engineering crops with improved drought tolerance. |
| 刊物名称: | The Plant Cell |
| 英文刊物名称: | The Plant Cell |
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| 其它备注: | Qian Chen, Ruijun Liu, Yaorong Wu, Shaowei Wei, Qian Wang, Yunna Zheng, Ran Xia, Xiaoling Shang, Feifei Yu, Xiaoyuan Yang, Lijing Liu, Xiahe Huang, Yingchun Wang and Qi Xie. ERAD-Related E2 and E3 Enzymes Modulate the Drought Response by Regulating the Stability of PIP2 Aquaporins. The Plant Cell. DOI:10.1093/plcell/koab141 |
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