Molecular Plant
Abstract
The genetic improvement of nitrogen use efficiency (NUE) of crops is vital for grain productivity and sustainable agriculture. However, the regulatory mechanism of NUE remains largely elusive. Here, we find that the riceGrain number, plant height, and heading date7(Ghd7) gene genetically acts upstream ofABC1 REPRESSOR1(ARE1), a negative regulator of NUE, to positively regulate nitrogen utilization. As a transcriptional repressor, Ghd7 directly binds to two Evening Element-like motifs in the promoter and Intron 1 ofARE1, likely in a cooperative manner, to repress its expression.Ghd7andARE1display diurnal expression patterns in an inverse oscillation manner, mirroring a regulatory scheme based on these two loci. Analysis of a panel of 2,656 rice varieties suggests that the elite alleles ofGhd7andARE1have undergone diversifying selection during breeding. Moreover, the allelic distribution ofGhd7andARE1is associated with the soil nitrogen deposition rate in East Asia and South Asia. Remarkably, the combination of theGhd7andARE1elite alleles substantially improves NUE and yield performance under nitrogen-limiting conditions. These results define aGhd7-ARE1-based mechanism regulating nitrogen utilization, which may represent useful targets for the genetic improvement of rice nitrogen utilization in breeding.
| 论文编号: | DOI:10.1016/j.molp.2021.04.012 |
| 论文题目: | The Ghd7 Transcription Factor Represses the ARE1 Expression to Enhance Nitrogen Utilization and Grain Yield in Rice |
| 英文论文题目: | The Ghd7 Transcription Factor Represses the ARE1 Expression to Enhance Nitrogen Utilization and Grain Yield in Rice |
| 第一作者: | Qing Wang, Qingmei Su, Jinqiang Nian, Jian Zhang, Meng Guo, Guojun Dong, Jiang Hu, Rongsheng Wang, Changshuo Wei, Guanwen Li, Wan Wang, Hui-Shan Guo, Shaoyang Lin, Wenfeng Qian, Xianzhi Xie, Qian Qian, Fan Chen, Jianru Zuo |
| 英文第一作者: | Qing Wang, Qingmei Su, Jinqiang Nian, Jian Zhang, Meng Guo, Guojun Dong, Jiang Hu, Rongsheng Wang, Changshuo Wei, Guanwen Li, Wan Wang, Hui-Shan Guo, Shaoyang Lin, Wenfeng Qian, Xianzhi Xie, Qian Qian, Fan Chen, Jianru Zuo |
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| 发表年度: | 2021-04-30 |
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| 摘要: | The genetic improvement of nitrogen use efficiency (NUE) of crops is vital for grain productivity and sustainable agriculture. However, the regulatory mechanism of NUE remains largely elusive. Here, we find that the riceGrain number, plant height, and heading date7(Ghd7) gene genetically acts upstream ofABC1 REPRESSOR1(ARE1), a negative regulator of NUE, to positively regulate nitrogen utilization. As a transcriptional repressor, Ghd7 directly binds to two Evening Element-like motifs in the promoter and Intron 1 ofARE1, likely in a cooperative manner, to repress its expression.Ghd7andARE1display diurnal expression patterns in an inverse oscillation manner, mirroring a regulatory scheme based on these two loci. Analysis of a panel of 2,656 rice varieties suggests that the elite alleles ofGhd7andARE1have undergone diversifying selection during breeding. Moreover, the allelic distribution ofGhd7andARE1is associated with the soil nitrogen deposition rate in East Asia and South Asia. Remarkably, the combination of theGhd7andARE1elite alleles substantially improves NUE and yield performance under nitrogen-limiting conditions. These results define aGhd7-ARE1-based mechanism regulating nitrogen utilization, which may represent useful targets for the genetic improvement of rice nitrogen utilization in breeding. |
| 英文摘要: | The genetic improvement of nitrogen use efficiency (NUE) of crops is vital for grain productivity and sustainable agriculture. However, the regulatory mechanism of NUE remains largely elusive. Here, we find that the riceGrain number, plant height, and heading date7(Ghd7) gene genetically acts upstream ofABC1 REPRESSOR1(ARE1), a negative regulator of NUE, to positively regulate nitrogen utilization. As a transcriptional repressor, Ghd7 directly binds to two Evening Element-like motifs in the promoter and Intron 1 ofARE1, likely in a cooperative manner, to repress its expression.Ghd7andARE1display diurnal expression patterns in an inverse oscillation manner, mirroring a regulatory scheme based on these two loci. Analysis of a panel of 2,656 rice varieties suggests that the elite alleles ofGhd7andARE1have undergone diversifying selection during breeding. Moreover, the allelic distribution ofGhd7andARE1is associated with the soil nitrogen deposition rate in East Asia and South Asia. Remarkably, the combination of theGhd7andARE1elite alleles substantially improves NUE and yield performance under nitrogen-limiting conditions. These results define aGhd7-ARE1-based mechanism regulating nitrogen utilization, which may represent useful targets for the genetic improvement of rice nitrogen utilization in breeding. |
| 刊物名称: | Molecular Plant |
| 英文刊物名称: | Molecular Plant |
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| 其它备注: | Qing Wang, Qingmei Su, Jinqiang Nian, Jian Zhang, Meng Guo, Guojun Dong, Jiang Hu, Rongsheng Wang, Changshuo Wei, Guanwen Li, Wan Wang, Hui-Shan Guo, Shaoyang Lin, Wenfeng Qian, Xianzhi Xie, Qian Qian, Fan Chen, Jianru Zuo. The Ghd7 Transcription Factor Represses the ARE1 Expression to Enhance Nitrogen Utilization and Grain Yield in Rice. Molecular Plant. DOI:10.1016/j.molp.2021.04.012 |
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