New Phytologist
Abstract
Soybean (Glycine max) is one of the most important oilseed crops. However, limited information is known about the regulatory mechanism of oil accumulation in soybean.
In this study, a member of tandem CCCH zinc finger (TZF) proteins, GmZF392, from our previous RNA‐seq analysis for seed‐preferred transcription factors, was found to function as a positive regulator of lipid production.
GmZF392 promotes seed oil accumulation in both transgenicArabidopsisand stable transgenic soybean plants by binding to a bipartitecis‐element containing a TG‐rich and a TA‐rich sequence in promoters and activates expression of genes in lipid biosynthesis pathway. GmZF392 physically interacts with GmZF351, our previously identified transcriptional regulator of lipid biosynthesis, to synergistically promote downstream gene expression. BothGmZF392andGmZF351are further up‐regulated by GmNFYA, another transcription factor in lipid biosynthesis, through direct and indirect manner, respectively. Promoter sequence diversity analysis showed that theGmZF392promoter may be selectively adopted at the origination of the genusGlycineand further mildly selected during domestication from wild soybeans to cultivated soybeans.
Our study reveals a regulatory module containing three transcription factors in lipid biosynthesis pathway, and manipulation of the module may improve oil production in soybean and other oilseed crops.
| 论文编号: | DOI:10.1111/nph.17401 |
| 论文题目: | A Transcriptional Regulatory Module Controls Lipid Accumulation in Soybean |
| 英文论文题目: | A Transcriptional Regulatory Module Controls Lipid Accumulation in Soybean |
| 第一作者: | Long Lu, Wei Wei, Qingtian Li, Xiaohua Bian, Xiang Lu, Yang Hu, Tong Cheng, Zhouya Wang, Meng Jin, Jianjun Tao, Cuicui Yin, Sijie He, Weiqun Man, Wei Li, Yongcai Lai, Wanke Zhang, Shouyi Chen, Jinsong Zhang |
| 英文第一作者: | Long Lu, Wei Wei, Qingtian Li, Xiaohua Bian, Xiang Lu, Yang Hu, Tong Cheng, Zhouya Wang, Meng Jin, Jianjun Tao, Cuicui Yin, Sijie He, Weiqun Man, Wei Li, Yongcai Lai, Wanke Zhang, Shouyi Chen, Jinsong Zhang |
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| 发表年度: | 2021-04-27 |
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| 摘要: | Soybean (Glycine max) is one of the most important oilseed crops. However, limited information is known about the regulatory mechanism of oil accumulation in soybean. In this study, a member of tandem CCCH zinc finger (TZF) proteins, GmZF392, from our previous RNA‐seq analysis for seed‐preferred transcription factors, was found to function as a positive regulator of lipid production. GmZF392 promotes seed oil accumulation in both transgenicArabidopsisand stable transgenic soybean plants by binding to a bipartitecis‐element containing a TG‐rich and a TA‐rich sequence in promoters and activates expression of genes in lipid biosynthesis pathway. GmZF392 physically interacts with GmZF351, our previously identified transcriptional regulator of lipid biosynthesis, to synergistically promote downstream gene expression. BothGmZF392andGmZF351are further up‐regulated by GmNFYA, another transcription factor in lipid biosynthesis, through direct and indirect manner, respectively. Promoter sequence diversity analysis showed that theGmZF392promoter may be selectively adopted at the origination of the genusGlycineand further mildly selected during domestication from wild soybeans to cultivated soybeans. Our study reveals a regulatory module containing three transcription factors in lipid biosynthesis pathway, and manipulation of the module may improve oil production in soybean and other oilseed crops. |
| 英文摘要: | Soybean (Glycine max) is one of the most important oilseed crops. However, limited information is known about the regulatory mechanism of oil accumulation in soybean. In this study, a member of tandem CCCH zinc finger (TZF) proteins, GmZF392, from our previous RNA‐seq analysis for seed‐preferred transcription factors, was found to function as a positive regulator of lipid production. GmZF392 promotes seed oil accumulation in both transgenicArabidopsisand stable transgenic soybean plants by binding to a bipartitecis‐element containing a TG‐rich and a TA‐rich sequence in promoters and activates expression of genes in lipid biosynthesis pathway. GmZF392 physically interacts with GmZF351, our previously identified transcriptional regulator of lipid biosynthesis, to synergistically promote downstream gene expression. BothGmZF392andGmZF351are further up‐regulated by GmNFYA, another transcription factor in lipid biosynthesis, through direct and indirect manner, respectively. Promoter sequence diversity analysis showed that theGmZF392promoter may be selectively adopted at the origination of the genusGlycineand further mildly selected during domestication from wild soybeans to cultivated soybeans. Our study reveals a regulatory module containing three transcription factors in lipid biosynthesis pathway, and manipulation of the module may improve oil production in soybean and other oilseed crops. |
| 刊物名称: | New Phytologist |
| 英文刊物名称: | New Phytologist |
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| 其它备注: | Long Lu, Wei Wei, Qingtian Li, Xiaohua Bian, Xiang Lu, Yang Hu, Tong Cheng, Zhouya Wang, Meng Jin, Jianjun Tao, Cuicui Yin, Sijie He, Weiqun Man, Wei Li, Yongcai Lai, Wanke Zhang, Shouyi Chen, Jinsong Zhang. A Transcriptional Regulatory Module Controls Lipid Accumulation in Soybean. New Phytologist. DOI:10.1111/nph.17401 |
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