New Phytologist
Abstract
Starch is the most abundant carbohydrate synthesized in plant chloroplast as the product of photosynthetic carbon assimilation, serving a crucial role in the carbon budget as storage energy. Phosphoglucose isomerase (PGI) catalyzes the interconversion between glucose 6‐phosphate (G6P) and fructose 6‐phosphate (F6P), which are important metabolic molecules in starch synthesis within chloroplasts and sucrose synthesis in cytosol.
Here, we found that the specific activity of recombinantly purified PGI localized in cytosol (PGIc) was much higher than its plastidic isoenzyme counterpart (PGIp) originated from wheat, rice and Arabidopsis, with wheat PGIc having by far the highest activity. Crystal structures of wheat TaPGIc and TaPGIp proteins were solved and the functional units were homodimers.
The active sites of PGIc and PGIp, constituted by the same amino acids, formed different binding pockets. Moreover, PGIc showed slightly lower affinity to the substrate F6P but with much faster turnover rates. Engineering of TaPGIc into chloroplasts of apgipmutant ofArabidopsis thaliana(atpgip) resulted in starch overaccumulation, increased carbon dioxide assimilation, up to 19% more plant biomass and 27% seed yield productivity.
These results show that manipulating starch metabolic pathways in chloroplasts can improve plant biomass and yield productivity.
| 论文编号: | DOI:10.1111/nph.17368 |
| 论文题目: | Engineering of the Cytosolic form of Phosphoglucose Isomerase into Chloroplasts Improves Plant Photosynthesis and Biomass |
| 英文论文题目: | Engineering of the Cytosolic form of Phosphoglucose Isomerase into Chloroplasts Improves Plant Photosynthesis and Biomass |
| 第一作者: | Fei Gao, Huijun Zhang, Wenjuan Zhang, Ning Wang, Shijia Zhang, Chengcai Chu, Cuimin Liu |
| 英文第一作者: | Fei Gao, Huijun Zhang, Wenjuan Zhang, Ning Wang, Shijia Zhang, Chengcai Chu, Cuimin Liu |
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| 发表年度: | 2021-04-02 |
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| 摘要: | Starch is the most abundant carbohydrate synthesized in plant chloroplast as the product of photosynthetic carbon assimilation, serving a crucial role in the carbon budget as storage energy. Phosphoglucose isomerase (PGI) catalyzes the interconversion between glucose 6‐phosphate (G6P) and fructose 6‐phosphate (F6P), which are important metabolic molecules in starch synthesis within chloroplasts and sucrose synthesis in cytosol. Here, we found that the specific activity of recombinantly purified PGI localized in cytosol (PGIc) was much higher than its plastidic isoenzyme counterpart (PGIp) originated from wheat, rice and Arabidopsis, with wheat PGIc having by far the highest activity. Crystal structures of wheat TaPGIc and TaPGIp proteins were solved and the functional units were homodimers. The active sites of PGIc and PGIp, constituted by the same amino acids, formed different binding pockets. Moreover, PGIc showed slightly lower affinity to the substrate F6P but with much faster turnover rates. Engineering of TaPGIc into chloroplasts of apgipmutant ofArabidopsis thaliana(atpgip) resulted in starch overaccumulation, increased carbon dioxide assimilation, up to 19% more plant biomass and 27% seed yield productivity. These results show that manipulating starch metabolic pathways in chloroplasts can improve plant biomass and yield productivity. |
| 英文摘要: | Starch is the most abundant carbohydrate synthesized in plant chloroplast as the product of photosynthetic carbon assimilation, serving a crucial role in the carbon budget as storage energy. Phosphoglucose isomerase (PGI) catalyzes the interconversion between glucose 6‐phosphate (G6P) and fructose 6‐phosphate (F6P), which are important metabolic molecules in starch synthesis within chloroplasts and sucrose synthesis in cytosol. Here, we found that the specific activity of recombinantly purified PGI localized in cytosol (PGIc) was much higher than its plastidic isoenzyme counterpart (PGIp) originated from wheat, rice and Arabidopsis, with wheat PGIc having by far the highest activity. Crystal structures of wheat TaPGIc and TaPGIp proteins were solved and the functional units were homodimers. The active sites of PGIc and PGIp, constituted by the same amino acids, formed different binding pockets. Moreover, PGIc showed slightly lower affinity to the substrate F6P but with much faster turnover rates. Engineering of TaPGIc into chloroplasts of apgipmutant ofArabidopsis thaliana(atpgip) resulted in starch overaccumulation, increased carbon dioxide assimilation, up to 19% more plant biomass and 27% seed yield productivity. These results show that manipulating starch metabolic pathways in chloroplasts can improve plant biomass and yield productivity. |
| 刊物名称: | New Phytologist |
| 英文刊物名称: | New Phytologist |
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| 其它备注: | Fei Gao, Huijun Zhang, Wenjuan Zhang, Ning Wang, Shijia Zhang, Chengcai Chu, Cuimin Liu. Engineering of the Cytosolic form of Phosphoglucose Isomerase into Chloroplasts Improves Plant Photosynthesis and Biomass. New Phytologist. DOI:10.1111/nph.17368 |
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