Nature Biotechnology
Abstract
Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30？°C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner.
| 论文编号: | DOI:10.1038/s41587-021-00868-w |
| 论文题目: | High-Efficiency Prime Editing with Optimized, Paired PegRNAs in Plants |
| 英文论文题目: | High-Efficiency Prime Editing with Optimized, Paired PegRNAs in Plants |
| 第一作者: | Qiupeng Lin, Shuai Jin, Yuan Zong, Hong Yu, Zixu Zhu, Guanwen Liu, Liquan Kou, Yanpeng Wang, Jin-Long Qiu, Jiayang Li & Caixia Gao |
| 英文第一作者: | Qiupeng Lin, Shuai Jin, Yuan Zong, Hong Yu, Zixu Zhu, Guanwen Liu, Liquan Kou, Yanpeng Wang, Jin-Long Qiu, Jiayang Li & Caixia Gao |
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| 发表年度: | 2021-03-26 |
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| 摘要: | Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30？°C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner. |
| 英文摘要: | Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30？°C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner. |
| 刊物名称: | Nature Biotechnology |
| 英文刊物名称: | Nature Biotechnology |
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| 其它备注: | Qiupeng Lin, Shuai Jin, Yuan Zong, Hong Yu, Zixu Zhu, Guanwen Liu, Liquan Kou, Yanpeng Wang, Jin-Long Qiu, Jiayang Li & Caixia Gao. High-Efficiency Prime Editing with Optimized, Paired PegRNAs in Plants. Nature Biotechnology. DOI:10.1038/s41587-021-00868-w |
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