Molecular Plant
Abstract
Grain number per panicle (GNP) is an important agronomic trait that contributes to rice grain yield. Despite its importance in rice breeding, the molecular mechanism underlying GNP regulation remains largely unknown. Here, we report that genetic variation in the CArG box-containing inverted repeat (IR) sequence of OsREM20 promoter alters its expression level and contributes to GNP variation among rice varieties through genetic analysis and transgenic validation. We revealed that the IR sequence regulates OsREM20 expression by affecting the direct binding of OsMADS34 to the CArG box within the IR sequence. The divergent pOsREM20IR and pOsREM20ΔIR alleles originated from different O. rufipogon accessions, and were independently inherited into the japonica and indica subspecies respectively during domestication. We further determined that IR sequence variations in the OsREM20 promoter can be utilized for germplasm improvement through either genome editing or traditional breeding. Our findings highlight exciting implications not only for characterizing the novel genetic variation that responsible for GNP diversity in rice, but also for revealing the molecular mechanism and potential application of the cis-regulatory element-containing IR sequence in the regulation of agronomically important gene expression.
| 论文编号: | DOI:10.1016/j.molp.2021.03.016 |
| 论文题目: | Enhancing Rice Grain Production by Manipulating Naturally Evolved cis-Regulatory Element-Containing Inverted Repeat Sequence of OsREM20 |
| 英文论文题目: | Enhancing Rice Grain Production by Manipulating Naturally Evolved cis-Regulatory Element-Containing Inverted Repeat Sequence of OsREM20 |
| 第一作者: | Xiaowei Wu, Yan Liang, Henbin Gao, Jiyao Wang, Yan Zhao, Lekai Hua, Yundong Yuan, Ahong Wang, Xiaohui Zhang, Jiafan Liu, Jie Zhou, Xiangbing Meng, Dahan Zhang, Shaoyang Lin, Xuehui Huang, Bin Han, Jiayang Li, Yonghong Wang |
| 英文第一作者: | Xiaowei Wu, Yan Liang, Henbin Gao, Jiyao Wang, Yan Zhao, Lekai Hua, Yundong Yuan, Ahong Wang, Xiaohui Zhang, Jiafan Liu, Jie Zhou, Xiangbing Meng, Dahan Zhang, Shaoyang Lin, Xuehui Huang, Bin Han, Jiayang Li, Yonghong Wang |
| 联系作者: | |
| 英文联系作者: | |
| 外单位作者单位: | |
| 英文外单位作者单位: | |
| 发表年度: | 2021-03-16 |
| 卷: | |
| 期: | |
| 页码: | |
| 摘要: | Grain number per panicle (GNP) is an important agronomic trait that contributes to rice grain yield. Despite its importance in rice breeding, the molecular mechanism underlying GNP regulation remains largely unknown. Here, we report that genetic variation in the CArG box-containing inverted repeat (IR) sequence of OsREM20 promoter alters its expression level and contributes to GNP variation among rice varieties through genetic analysis and transgenic validation. We revealed that the IR sequence regulates OsREM20 expression by affecting the direct binding of OsMADS34 to the CArG box within the IR sequence. The divergent pOsREM20IR and pOsREM20ΔIR alleles originated from different O. rufipogon accessions, and were independently inherited into the japonica and indica subspecies respectively during domestication. We further determined that IR sequence variations in the OsREM20 promoter can be utilized for germplasm improvement through either genome editing or traditional breeding. Our findings highlight exciting implications not only for characterizing the novel genetic variation that responsible for GNP diversity in rice, but also for revealing the molecular mechanism and potential application of the cis-regulatory element-containing IR sequence in the regulation of agronomically important gene expression. |
| 英文摘要: | Grain number per panicle (GNP) is an important agronomic trait that contributes to rice grain yield. Despite its importance in rice breeding, the molecular mechanism underlying GNP regulation remains largely unknown. Here, we report that genetic variation in the CArG box-containing inverted repeat (IR) sequence of OsREM20 promoter alters its expression level and contributes to GNP variation among rice varieties through genetic analysis and transgenic validation. We revealed that the IR sequence regulates OsREM20 expression by affecting the direct binding of OsMADS34 to the CArG box within the IR sequence. The divergent pOsREM20IR and pOsREM20ΔIR alleles originated from different O. rufipogon accessions, and were independently inherited into the japonica and indica subspecies respectively during domestication. We further determined that IR sequence variations in the OsREM20 promoter can be utilized for germplasm improvement through either genome editing or traditional breeding. Our findings highlight exciting implications not only for characterizing the novel genetic variation that responsible for GNP diversity in rice, but also for revealing the molecular mechanism and potential application of the cis-regulatory element-containing IR sequence in the regulation of agronomically important gene expression. |
| 刊物名称: | Molecular Plant |
| 英文刊物名称: | Molecular Plant |
| 论文全文: | |
| 英文论文全文: | |
| 全文链接: | |
| 其它备注: | Xiaowei Wu, Yan Liang, Henbin Gao, Jiyao Wang, Yan Zhao, Lekai Hua, Yundong Yuan, Ahong Wang, Xiaohui Zhang, Jiafan Liu, Jie Zhou, Xiangbing Meng, Dahan Zhang, Shaoyang Lin, Xuehui Huang, Bin Han, Jiayang Li, Yonghong Wang. Enhancing Rice Grain Production by Manipulating Naturally Evolved cis-Regulatory Element-Containing Inverted Repeat Sequence of OsREM20. Molecular Plant. DOI:10.1016/j.molp.2021.03.016 |
| 英文其它备注: | |
| 学科: | |
| 英文学科: | |
| 影响因子: | |
| 第一作者所在部门: | |
| 英文第一作者所在部门: | |
| 论文出处: | |
| 英文论文出处: | |
| 论文类别: | |
| 英文论文类别: | |
| 参与作者: | |
| 英文参与作者: |
