New phytologist
Abstract
The bipolar spindle structure in meiosis is essential for faithful chromosome segregation. PUTATIVE RECOMBINATION INITIATION DEFECT 1 (PRD1) has been previously shown to participate in the formation of DNA double strand breaks (DSBs). However, the role of PRD1 in meiotic spindle assembly has not been elucidated.
Here, we reveal by both genetic analysis and immunostaining technology that PRD1 is involved in spindle assembly in rice (Oryza sativa) meiosis.
We show that DSB formation and bipolar spindle assembly are disturbed inprd1meiocytes. PRD1 signals display a dynamic pattern of localization from covering entire chromosomes at leptotene to congregating at the centromere region after leptotene. Centromeric localization of PRD1 signals depends on the organization of leptotene chromosomes, but not on DSB formation and axis establishment. PRD1 exhibits interaction and co‐localization with several kinetochore components. We also find bi‐orientation of sister kinetochores within a univalent induced by mutation of REC8 can restore bipolarity inprd1. Furthermore, PRD1 directly interacts with REC8 and SGO1, suggesting that PRD1 may play a role in regulating the orientation of sister kinetochores.
Taken together, we speculate that PRD1 promotes bipolar spindle assembly, presumably by modulating the orientation of sister kinetochores in rice meiosis.
| 论文编号: | DOI:10.1111/nph.17178 |
| 论文题目: | PRD1, a homologous recombination initiation factor, is involved in spindle assembly in rice meiosis |
| 英文论文题目: | PRD1, a homologous recombination initiation factor, is involved in spindle assembly in rice meiosis |
| 第一作者: | Wenqing Shi, Jianhui Ji, Zhihui Xue, Fanfan Zhang, Yongjie Miao, Han Yang, Ding Tang, Guijie Du, Yafei Li, Yi Shen, Zhukuan Cheng |
| 英文第一作者: | Wenqing Shi, Jianhui Ji, Zhihui Xue, Fanfan Zhang, Yongjie Miao, Han Yang, Ding Tang, Guijie Du, Yafei Li, Yi Shen, Zhukuan Cheng |
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| 发表年度: | 2021-01-13 |
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| 摘要: | The bipolar spindle structure in meiosis is essential for faithful chromosome segregation. PUTATIVE RECOMBINATION INITIATION DEFECT 1 (PRD1) has been previously shown to participate in the formation of DNA double strand breaks (DSBs). However, the role of PRD1 in meiotic spindle assembly has not been elucidated. Here, we reveal by both genetic analysis and immunostaining technology that PRD1 is involved in spindle assembly in rice (Oryza sativa) meiosis. We show that DSB formation and bipolar spindle assembly are disturbed inprd1meiocytes. PRD1 signals display a dynamic pattern of localization from covering entire chromosomes at leptotene to congregating at the centromere region after leptotene. Centromeric localization of PRD1 signals depends on the organization of leptotene chromosomes, but not on DSB formation and axis establishment. PRD1 exhibits interaction and co‐localization with several kinetochore components. We also find bi‐orientation of sister kinetochores within a univalent induced by mutation of REC8 can restore bipolarity inprd1. Furthermore, PRD1 directly interacts with REC8 and SGO1, suggesting that PRD1 may play a role in regulating the orientation of sister kinetochores. Taken together, we speculate that PRD1 promotes bipolar spindle assembly, presumably by modulating the orientation of sister kinetochores in rice meiosis. |
| 英文摘要: | The bipolar spindle structure in meiosis is essential for faithful chromosome segregation. PUTATIVE RECOMBINATION INITIATION DEFECT 1 (PRD1) has been previously shown to participate in the formation of DNA double strand breaks (DSBs). However, the role of PRD1 in meiotic spindle assembly has not been elucidated. Here, we reveal by both genetic analysis and immunostaining technology that PRD1 is involved in spindle assembly in rice (Oryza sativa) meiosis. We show that DSB formation and bipolar spindle assembly are disturbed inprd1meiocytes. PRD1 signals display a dynamic pattern of localization from covering entire chromosomes at leptotene to congregating at the centromere region after leptotene. Centromeric localization of PRD1 signals depends on the organization of leptotene chromosomes, but not on DSB formation and axis establishment. PRD1 exhibits interaction and co‐localization with several kinetochore components. We also find bi‐orientation of sister kinetochores within a univalent induced by mutation of REC8 can restore bipolarity inprd1. Furthermore, PRD1 directly interacts with REC8 and SGO1, suggesting that PRD1 may play a role in regulating the orientation of sister kinetochores. Taken together, we speculate that PRD1 promotes bipolar spindle assembly, presumably by modulating the orientation of sister kinetochores in rice meiosis. |
| 刊物名称: | New phytologist |
| 英文刊物名称: | New phytologist |
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| 其它备注: | Wenqing Shi, Jianhui Ji, Zhihui Xue, Fanfan Zhang, Yongjie Miao, Han Yang, Ding Tang, Guijie Du, Yafei Li, Yi Shen, Zhukuan Cheng. PRD1, a homologous recombination initiation factor, is involved in spindle assembly in rice meiosis. New phytologist. DOI:10.1111/nph.17178 |
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