Molecular Plant
Abstract
The coordinated utilization of nitrogen (N) and phosphorus (P) is vital to maintain nutrient balance and achieve an optimal growth for plants. Previously, we revealed a mechanism by which nitrate induces genes for phosphate utilization depending on NRT1.1B-facilitated degradation of cytoplasmic SPX4, thus promoting cytoplasmic-nuclear shuttling of PHR2, the central transcription factor of phosphate signaling, and triggering nitrate-induced phosphate response (NIPR) and N-P coordinated utilization in rice. Here we further unveil a working mechanism for fine-tuning NIPR in nucleus by Highly Induced by Nitrate Gene 1 (HINGE1, also known as RLI1), a MYB-transcription factor closely clustered to PHR2. RLI1/HINGE1 is transcriptionally activated by PHR2 under nitrate induction and can directly activate the expression of phosphate starvation induced genes. More importantly, RLI1/HINGE1 competes with PHR2 to bind its repressor proteins in nucleus (SPX proteins), and consequently releases PHR2 to further enhance phosphate response. Therefore, RLI1/HINGE1 amplifies the phosphate response in nucleus after cytoplasmic SPX4-PHR2 cascade, thereby enabling a fine-tuning of N-P balance under sufficient supply of nitrate.
| 论文编号: | DOI:10.1016/j.molp.2020.12.005 |
| 论文题目: | Modulation of Nitrate-Induced Phosphate Response by RLI1/HINGE1 in Nucleus |
| 英文论文题目: | Modulation of Nitrate-Induced Phosphate Response by RLI1/HINGE1 in Nucleus |
| 第一作者: | Zhihua Zhang, Zhao Li, Wei Wang, Zhimin Jiang, Liping Guo, Xiaohan Wang, Yangwen Qian, Xiahe Huang, Yongqiang Liu, Xiujie Liu, Yahong Qiu, Aifu Li, Yu Yan, Junpeng Xie, Shouyun Cao, Stanislav Kopriva, Legong Li, Fanjiang Kong, Baohui Liu, Yingchun Wang, Bin Hu, Chengcai Chu |
| 英文第一作者: | Zhihua Zhang, Zhao Li, Wei Wang, Zhimin Jiang, Liping Guo, Xiaohan Wang, Yangwen Qian, Xiahe Huang, Yongqiang Liu, Xiujie Liu, Yahong Qiu, Aifu Li, Yu Yan, Junpeng Xie, Shouyun Cao, Stanislav Kopriva, Legong Li, Fanjiang Kong, Baohui Liu, Yingchun Wang, Bin Hu, Chengcai Chu |
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| 发表年度: | 2020-12-14 |
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| 摘要: | The coordinated utilization of nitrogen (N) and phosphorus (P) is vital to maintain nutrient balance and achieve an optimal growth for plants. Previously, we revealed a mechanism by which nitrate induces genes for phosphate utilization depending on NRT1.1B-facilitated degradation of cytoplasmic SPX4, thus promoting cytoplasmic-nuclear shuttling of PHR2, the central transcription factor of phosphate signaling, and triggering nitrate-induced phosphate response (NIPR) and N-P coordinated utilization in rice. Here we further unveil a working mechanism for fine-tuning NIPR in nucleus by Highly Induced by Nitrate Gene 1 (HINGE1, also known as RLI1), a MYB-transcription factor closely clustered to PHR2. RLI1/HINGE1 is transcriptionally activated by PHR2 under nitrate induction and can directly activate the expression of phosphate starvation induced genes. More importantly, RLI1/HINGE1 competes with PHR2 to bind its repressor proteins in nucleus (SPX proteins), and consequently releases PHR2 to further enhance phosphate response. Therefore, RLI1/HINGE1 amplifies the phosphate response in nucleus after cytoplasmic SPX4-PHR2 cascade, thereby enabling a fine-tuning of N-P balance under sufficient supply of nitrate. |
| 英文摘要: | The coordinated utilization of nitrogen (N) and phosphorus (P) is vital to maintain nutrient balance and achieve an optimal growth for plants. Previously, we revealed a mechanism by which nitrate induces genes for phosphate utilization depending on NRT1.1B-facilitated degradation of cytoplasmic SPX4, thus promoting cytoplasmic-nuclear shuttling of PHR2, the central transcription factor of phosphate signaling, and triggering nitrate-induced phosphate response (NIPR) and N-P coordinated utilization in rice. Here we further unveil a working mechanism for fine-tuning NIPR in nucleus by Highly Induced by Nitrate Gene 1 (HINGE1, also known as RLI1), a MYB-transcription factor closely clustered to PHR2. RLI1/HINGE1 is transcriptionally activated by PHR2 under nitrate induction and can directly activate the expression of phosphate starvation induced genes. More importantly, RLI1/HINGE1 competes with PHR2 to bind its repressor proteins in nucleus (SPX proteins), and consequently releases PHR2 to further enhance phosphate response. Therefore, RLI1/HINGE1 amplifies the phosphate response in nucleus after cytoplasmic SPX4-PHR2 cascade, thereby enabling a fine-tuning of N-P balance under sufficient supply of nitrate. |
| 刊物名称: | Molecular Plant |
| 英文刊物名称: | Molecular Plant |
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| 其它备注: | Zhihua Zhang, Zhao Li, Wei Wang, Zhimin Jiang, Liping Guo, Xiaohan Wang, Yangwen Qian, Xiahe Huang, Yongqiang Liu, Xiujie Liu, Yahong Qiu, Aifu Li, Yu Yan, Junpeng Xie, Shouyun Cao, Stanislav Kopriva, Legong Li, Fanjiang Kong, Baohui Liu, Yingchun Wang, Bin Hu, Chengcai Chu. Modulation of Nitrate-Induced Phosphate Response by RLI1/HINGE1 in Nucleus. Molecular Plant. DOI:10.1016/j.molp.2020.12.005 |
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