The Plant Journal
Abstract
Two branching strategies are exhibited in crops: enhanced apical dominance, as in maize, or weak apical dominance, as in rice. However, the underlying mechanism of weak apical dominance remains elusive. OsWUS, an ortholog of Arabidopsis WUSCHEL (WUS) in rice, is required for tiller development. In this study, we identified and functionally characterized a low-tillering mutant decreased culm number 1 (dc1) that resulted from loss-of-function of OsWUS. The dc1 tiller buds are viable but repressed by the main culm apex, leading to stronger apical dominance than that of the wild type. Auxin response is enhanced in the dc1 mutant and knocking out the auxin action-associated gene ABERRANT SPIKELET AND PANICLE 1 (ASP1) de-repressed growth of the tiller buds in the dc1 mutant, suggesting that OsWUS and ASP1 are both involved in outgrowth of the rice tiller bud. Decapitation triggers higher contents of cytokinins in the shoot base of the dc1 mutant compared with those in the wild type, and exogenous application of cytokinin is not sufficient for sustained growth of the dc1 tiller bud. Transcriptome analysis indicated that expression levels of transcription factors putatively bound by ORYZA SATIVA HOMEOBOX 1 (OSH1) are changed in response to decapitation and display a greater fold change in the dc1 mutant than that in the wild type. Collectively, these findings reveal an important role of OsWUS in tiller bud growth by influencing apical dominance and provide the basis for an improved understanding of tiller bud development in rice.
| 论文编号: | DOI:10.1111/tpj.15026 |
| 论文题目: | OsWUS Promotes Tiller Bud Growth by Establishing Weak Apical Dominance in Rice |
| 英文论文题目: | OsWUS Promotes Tiller Bud Growth by Establishing Weak Apical Dominance in Rice |
| 第一作者: | Tianyu Xia, Hongqi Chen, Sujun Dong, Zeyang Ma, Haibo Ren, Xudong Zhu, Xiaohua Fang, Fan Chen |
| 英文第一作者: | Tianyu Xia, Hongqi Chen, Sujun Dong, Zeyang Ma, Haibo Ren, Xudong Zhu, Xiaohua Fang, Fan Chen |
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| 发表年度: | 2020-10-29 |
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| 摘要: | Two branching strategies are exhibited in crops: enhanced apical dominance, as in maize, or weak apical dominance, as in rice. However, the underlying mechanism of weak apical dominance remains elusive. OsWUS, an ortholog of Arabidopsis WUSCHEL (WUS) in rice, is required for tiller development. In this study, we identified and functionally characterized a low-tillering mutant decreased culm number 1 (dc1) that resulted from loss-of-function of OsWUS. The dc1 tiller buds are viable but repressed by the main culm apex, leading to stronger apical dominance than that of the wild type. Auxin response is enhanced in the dc1 mutant and knocking out the auxin action-associated gene ABERRANT SPIKELET AND PANICLE 1 (ASP1) de-repressed growth of the tiller buds in the dc1 mutant, suggesting that OsWUS and ASP1 are both involved in outgrowth of the rice tiller bud. Decapitation triggers higher contents of cytokinins in the shoot base of the dc1 mutant compared with those in the wild type, and exogenous application of cytokinin is not sufficient for sustained growth of the dc1 tiller bud. Transcriptome analysis indicated that expression levels of transcription factors putatively bound by ORYZA SATIVA HOMEOBOX 1 (OSH1) are changed in response to decapitation and display a greater fold change in the dc1 mutant than that in the wild type. Collectively, these findings reveal an important role of OsWUS in tiller bud growth by influencing apical dominance and provide the basis for an improved understanding of tiller bud development in rice. |
| 英文摘要: | Two branching strategies are exhibited in crops: enhanced apical dominance, as in maize, or weak apical dominance, as in rice. However, the underlying mechanism of weak apical dominance remains elusive. OsWUS, an ortholog of Arabidopsis WUSCHEL (WUS) in rice, is required for tiller development. In this study, we identified and functionally characterized a low-tillering mutant decreased culm number 1 (dc1) that resulted from loss-of-function of OsWUS. The dc1 tiller buds are viable but repressed by the main culm apex, leading to stronger apical dominance than that of the wild type. Auxin response is enhanced in the dc1 mutant and knocking out the auxin action-associated gene ABERRANT SPIKELET AND PANICLE 1 (ASP1) de-repressed growth of the tiller buds in the dc1 mutant, suggesting that OsWUS and ASP1 are both involved in outgrowth of the rice tiller bud. Decapitation triggers higher contents of cytokinins in the shoot base of the dc1 mutant compared with those in the wild type, and exogenous application of cytokinin is not sufficient for sustained growth of the dc1 tiller bud. Transcriptome analysis indicated that expression levels of transcription factors putatively bound by ORYZA SATIVA HOMEOBOX 1 (OSH1) are changed in response to decapitation and display a greater fold change in the dc1 mutant than that in the wild type. Collectively, these findings reveal an important role of OsWUS in tiller bud growth by influencing apical dominance and provide the basis for an improved understanding of tiller bud development in rice. |
| 刊物名称: | The Plant Journal |
| 英文刊物名称: | The Plant Journal |
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| 其它备注: | Tianyu Xia, Hongqi Chen, Sujun Dong, Zeyang Ma, Haibo Ren, Xudong Zhu, Xiaohua Fang, Fan Chen. OsWUS Promotes Tiller Bud Growth by Establishing Weak Apical Dominance in Rice.The Plant Journal. DOI:10.1111/tpj.15026 |
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