Molecular Plant
Abstract
Trichomes are universally present in plants and trichome development is delicately regulated. Trichomes are responsible for pubescence, and their density is associated with agronomical traits related to insect resistance, evapotranspiration and yield. Almost a century ago, three dominant alleles related to pubescence density in soybean, namelyPd1(dense pubescence),Ps(sparse pubescence) andP1(glabrous), were identified. However, their identity and inheritance remain unclear. Here, through genome-wide association study (GWAS) and map-based cloning, we determined the genetic basis of these three traits. The sparse pubescence phenotype ofPswas attributed to a copy number variation (CNV) of a 25.6-kb sequence that includes a gene encoding a protein with WD40 and RING domains, the dense pubescence phenotype ofPd1was attributed to a T-C transition in the last exon of a HD-Zip transcription factor gene, and the glabrous phenotype ofP1was attributed to a G-A transition in the first exon of a lipid transfer protein gene. Genetic and biochemical analyses revealed that Pd1 functions as a transcriptional activator that binds to the promoters of theP1andPsgenes to induce their expression and that binds to its own promoter to act as a transcriptional inhibitor. In addition, Ps can interact with Pd1 and weaken the transcriptional activity of Pd1. Taken together, our results demonstrate thatPd1,Ps, andP1form a complex feedback loop to regulate pubescence formation.
| 论文编号: | DOI:10.1016/j.molp.2020.10.004 |
| 论文题目: | A Pd1-Ps-P1 Feedback Loop Controls Pubescence Density in Soybean |
| 英文论文题目: | A Pd1-Ps-P1 Feedback Loop Controls Pubescence Density in Soybean |
| 第一作者: | Shulin Liu, Lei Fan, Zhi Liu, Xia Yang, Zhifang Zhang, Zongbiao Duan, Qianjin Liang, Muhammad Imran, Min Zhang, Zhixi Tian |
| 英文第一作者: | Shulin Liu, Lei Fan, Zhi Liu, Xia Yang, Zhifang Zhang, Zongbiao Duan, Qianjin Liang, Muhammad Imran, Min Zhang, Zhixi Tian |
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| 发表年度: | 2020-10-20 |
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| 摘要: | Trichomes are universally present in plants and trichome development is delicately regulated. Trichomes are responsible for pubescence, and their density is associated with agronomical traits related to insect resistance, evapotranspiration and yield. Almost a century ago, three dominant alleles related to pubescence density in soybean, namelyPd1(dense pubescence),Ps(sparse pubescence) andP1(glabrous), were identified. However, their identity and inheritance remain unclear. Here, through genome-wide association study (GWAS) and map-based cloning, we determined the genetic basis of these three traits. The sparse pubescence phenotype ofPswas attributed to a copy number variation (CNV) of a 25.6-kb sequence that includes a gene encoding a protein with WD40 and RING domains, the dense pubescence phenotype ofPd1was attributed to a T-C transition in the last exon of a HD-Zip transcription factor gene, and the glabrous phenotype ofP1was attributed to a G-A transition in the first exon of a lipid transfer protein gene. Genetic and biochemical analyses revealed that Pd1 functions as a transcriptional activator that binds to the promoters of theP1andPsgenes to induce their expression and that binds to its own promoter to act as a transcriptional inhibitor. In addition, Ps can interact with Pd1 and weaken the transcriptional activity of Pd1. Taken together, our results demonstrate thatPd1,Ps, andP1form a complex feedback loop to regulate pubescence formation. |
| 英文摘要: | Trichomes are universally present in plants and trichome development is delicately regulated. Trichomes are responsible for pubescence, and their density is associated with agronomical traits related to insect resistance, evapotranspiration and yield. Almost a century ago, three dominant alleles related to pubescence density in soybean, namelyPd1(dense pubescence),Ps(sparse pubescence) andP1(glabrous), were identified. However, their identity and inheritance remain unclear. Here, through genome-wide association study (GWAS) and map-based cloning, we determined the genetic basis of these three traits. The sparse pubescence phenotype ofPswas attributed to a copy number variation (CNV) of a 25.6-kb sequence that includes a gene encoding a protein with WD40 and RING domains, the dense pubescence phenotype ofPd1was attributed to a T-C transition in the last exon of a HD-Zip transcription factor gene, and the glabrous phenotype ofP1was attributed to a G-A transition in the first exon of a lipid transfer protein gene. Genetic and biochemical analyses revealed that Pd1 functions as a transcriptional activator that binds to the promoters of theP1andPsgenes to induce their expression and that binds to its own promoter to act as a transcriptional inhibitor. In addition, Ps can interact with Pd1 and weaken the transcriptional activity of Pd1. Taken together, our results demonstrate thatPd1,Ps, andP1form a complex feedback loop to regulate pubescence formation. |
| 刊物名称: | Molecular Plant |
| 英文刊物名称: | Molecular Plant |
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| 其它备注: | Shulin Liu, Lei Fan, Zhi Liu, Xia Yang, Zhifang Zhang, Zongbiao Duan, Qianjin Liang, Muhammad Imran, Min Zhang, Zhixi Tian. A Pd1-Ps-P1 Feedback Loop Controls Pubescence Density in Soybean. Molecular Plant. DOI:10.1016/j.molp.2020.10.004 |
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