Plant Physiology
Abstract
ATAXIA TELANGIECTASIA-MUTATED (ATM) protein has been well studied for its roles in the DNA damage response. However, its role in meiosis has not been fully explored. Here, we characterized the functions of the rice (Oryza sativa) ATM homolog during meiosis. Aberrant chromosome associations and DNA fragmentations were observed after the completion of homologous pairing and synapsis in Osatm pollen mother cells (PMCs). Aberrant chromosome associations disappeared in Osspo11-1 Osatm-1 double mutants and more severe defects were observed in Osdmc1 Osatm, suggesting that OsATM functions downstream of OsSPO11-1-catalyzed double-strand break (DSB) formation and in parallel with OsDMC1-mediated homologous recombination. We further demonstrated that phosphorylation of H2AX in PMCs did not depend on OsATM, in contrast to the situation in somatic cells. Moreover, the removal of OsDMC1 from chromosomes in Osatm PMCs was delayed and the number of HEI10 foci (markers of interference-sensitive CO intermediates) decreased. Together, these findings suggest that OsATM plays important roles in the accurate repair of meiotic DSBs in rice.
| 论文编号: | DOI:10.1104/pp.20.00053 |
| 论文题目: | OsATM Safeguards Accurate Repair of Meiotic Double-strand Breaks in Rice |
| 英文论文题目: | OsATM Safeguards Accurate Repair of Meiotic Double-strand Breaks in Rice |
| 第一作者: | Chao Zhang, Fanfan Zhang, Xinjie Cheng, Kangwei Liu, Jiaqi Tang, Yafei Li, DingTang, Zhukuan Cheng, Hengxiu Yu |
| 英文第一作者: | Chao Zhang, Fanfan Zhang, Xinjie Cheng, Kangwei Liu, Jiaqi Tang, Yafei Li, DingTang, Zhukuan Cheng, Hengxiu Yu |
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| 发表年度: | 2020-05-26 |
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| 摘要: | ATAXIA TELANGIECTASIA-MUTATED (ATM) protein has been well studied for its roles in the DNA damage response. However, its role in meiosis has not been fully explored. Here, we characterized the functions of the rice (Oryza sativa) ATM homolog during meiosis. Aberrant chromosome associations and DNA fragmentations were observed after the completion of homologous pairing and synapsis in Osatm pollen mother cells (PMCs). Aberrant chromosome associations disappeared in Osspo11-1 Osatm-1 double mutants and more severe defects were observed in Osdmc1 Osatm, suggesting that OsATM functions downstream of OsSPO11-1-catalyzed double-strand break (DSB) formation and in parallel with OsDMC1-mediated homologous recombination. We further demonstrated that phosphorylation of H2AX in PMCs did not depend on OsATM, in contrast to the situation in somatic cells. Moreover, the removal of OsDMC1 from chromosomes in Osatm PMCs was delayed and the number of HEI10 foci (markers of interference-sensitive CO intermediates) decreased. Together, these findings suggest that OsATM plays important roles in the accurate repair of meiotic DSBs in rice. |
| 英文摘要: | ATAXIA TELANGIECTASIA-MUTATED (ATM) protein has been well studied for its roles in the DNA damage response. However, its role in meiosis has not been fully explored. Here, we characterized the functions of the rice (Oryza sativa) ATM homolog during meiosis. Aberrant chromosome associations and DNA fragmentations were observed after the completion of homologous pairing and synapsis in Osatm pollen mother cells (PMCs). Aberrant chromosome associations disappeared in Osspo11-1 Osatm-1 double mutants and more severe defects were observed in Osdmc1 Osatm, suggesting that OsATM functions downstream of OsSPO11-1-catalyzed double-strand break (DSB) formation and in parallel with OsDMC1-mediated homologous recombination. We further demonstrated that phosphorylation of H2AX in PMCs did not depend on OsATM, in contrast to the situation in somatic cells. Moreover, the removal of OsDMC1 from chromosomes in Osatm PMCs was delayed and the number of HEI10 foci (markers of interference-sensitive CO intermediates) decreased. Together, these findings suggest that OsATM plays important roles in the accurate repair of meiotic DSBs in rice. |
| 刊物名称: | Plant Physiology |
| 英文刊物名称: | Plant Physiology |
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| 其它备注: | Chao Zhang, Fanfan Zhang, Xinjie Cheng, Kangwei Liu, Jiaqi Tang, Yafei Li, Ding Tang, Zhukuan Cheng, Hengxiu Yu. OsATM Safeguards Accurate Repair of Meiotic Double-strand Breaks in Rice. Plant Physiology. DOI:10.1104/pp.20.00053 |
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