The Plant Cell
Abstract
Strigolactones (SLs) and karrikins (KARs) are related butenolide signaling molecules that control plant development. In Arabidopsis thaliana they are recognized separately by two closely related receptors but employ the same F-box protein MORE AXILLARY GROWTH2 (MAX2) for signal transduction, targeting different members of the SMAX1-LIKE (SMXL) family of transcriptional repressors for degradation. Both signals inhibit hypocotyl elongation in seedlings, raising the question of whether signaling is convergent or parallel. Here, we show that synthetic SL analog GR244DO enhanced the interaction between the SL receptor DWARF14 (D14) and SMXL2, while the KAR surrogate GR24ent-5DS induced association of the KAR receptor KARRIKIN INSENSITIVE2 (KAI2) with SMAX1 and SMXL2. Both signals trigger polyubiquitination and degradation of SMXL2, with GR244DO dependent on D14 and GR24ent-5DS dependent mainly on KAI2. SMXL2 is critical for hypocotyl responses to GR244DO, and functions redundantly with SMAX1 in hypocotyl response to GR24ent-5DS. Furthermore, GR244DO induced response of D14-LIKE2 (DLK2) and KAR-UP F-BOX1 (KUF1) through SMXL2, whereas GR24ent-5DS induced expression of these genes via both SMAX1 and SMXL2. These findings demonstrate that both SLs and KARs could trigger polyubiquitination and degradation of SMXL2, thus uncovering an unexpected but important convergent pathway in SL- and KAR-regulated gene expression and hypocotyl elongation.
| 论文编号: | DOI:10.1105/tpc.20.00140 |
| 论文题目: | Strigolactone and Karrikin Signaling Pathways Elicit Ubiquitination and Proteolysis of SMXL2 to Regulate Hypocotyl Elongation in Arabidopsis thaliana |
| 英文论文题目: | Strigolactone and Karrikin Signaling Pathways Elicit Ubiquitination and Proteolysis of SMXL2 to Regulate Hypocotyl Elongation in Arabidopsis thaliana |
| 第一作者: | Lei Wang, Qian Xu, Hong Yu, Haiyan Ma, Xiaoqiang Li, Jun Yang, Jinfang Chu, Qi Xie, Yonghong Wang, Steven M. Smith, Jiayang Li, Guosheng Xiong, Bing Wang |
| 英文第一作者: | Lei Wang, Qian Xu, Hong Yu, Haiyan Ma, Xiaoqiang Li, Jun Yang, Jinfang Chu, Qi Xie, Yonghong Wang, Steven M. Smith, Jiayang Li, Guosheng Xiong, Bing Wang |
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| 发表年度: | 2020-05-07 |
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| 摘要: | Strigolactones (SLs) and karrikins (KARs) are related butenolide signaling molecules that control plant development. In Arabidopsis thaliana they are recognized separately by two closely related receptors but employ the same F-box protein MORE AXILLARY GROWTH2 (MAX2) for signal transduction, targeting different members of the SMAX1-LIKE (SMXL) family of transcriptional repressors for degradation. Both signals inhibit hypocotyl elongation in seedlings, raising the question of whether signaling is convergent or parallel. Here, we show that synthetic SL analog GR244DO enhanced the interaction between the SL receptor DWARF14 (D14) and SMXL2, while the KAR surrogate GR24ent-5DS induced association of the KAR receptor KARRIKIN INSENSITIVE2 (KAI2) with SMAX1 and SMXL2. Both signals trigger polyubiquitination and degradation of SMXL2, with GR244DO dependent on D14 and GR24ent-5DS dependent mainly on KAI2. SMXL2 is critical for hypocotyl responses to GR244DO, and functions redundantly with SMAX1 in hypocotyl response to GR24ent-5DS. Furthermore, GR244DO induced response of D14-LIKE2 (DLK2) and KAR-UP F-BOX1 (KUF1) through SMXL2, whereas GR24ent-5DS induced expression of these genes via both SMAX1 and SMXL2. These findings demonstrate that both SLs and KARs could trigger polyubiquitination and degradation of SMXL2, thus uncovering an unexpected but important convergent pathway in SL- and KAR-regulated gene expression and hypocotyl elongation. |
| 英文摘要: | Strigolactones (SLs) and karrikins (KARs) are related butenolide signaling molecules that control plant development. In Arabidopsis thaliana they are recognized separately by two closely related receptors but employ the same F-box protein MORE AXILLARY GROWTH2 (MAX2) for signal transduction, targeting different members of the SMAX1-LIKE (SMXL) family of transcriptional repressors for degradation. Both signals inhibit hypocotyl elongation in seedlings, raising the question of whether signaling is convergent or parallel. Here, we show that synthetic SL analog GR244DO enhanced the interaction between the SL receptor DWARF14 (D14) and SMXL2, while the KAR surrogate GR24ent-5DS induced association of the KAR receptor KARRIKIN INSENSITIVE2 (KAI2) with SMAX1 and SMXL2. Both signals trigger polyubiquitination and degradation of SMXL2, with GR244DO dependent on D14 and GR24ent-5DS dependent mainly on KAI2. SMXL2 is critical for hypocotyl responses to GR244DO, and functions redundantly with SMAX1 in hypocotyl response to GR24ent-5DS. Furthermore, GR244DO induced response of D14-LIKE2 (DLK2) and KAR-UP F-BOX1 (KUF1) through SMXL2, whereas GR24ent-5DS induced expression of these genes via both SMAX1 and SMXL2. These findings demonstrate that both SLs and KARs could trigger polyubiquitination and degradation of SMXL2, thus uncovering an unexpected but important convergent pathway in SL- and KAR-regulated gene expression and hypocotyl elongation. |
| 刊物名称: | The Plant Cell |
| 英文刊物名称: | The Plant Cell |
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| 其它备注: | Lei Wang, Qian Xu, Hong Yu, Haiyan Ma, Xiaoqiang Li, Jun Yang, Jinfang Chu, Qi Xie, Yonghong Wang, Steven M. Smith, Jiayang Li, Guosheng Xiong, Bing Wang. Strigolactone and Karrikin Signaling Pathways Elicit Ubiquitination and Proteolysis of SMXL2 to Regulate Hypocotyl Elongation in Arabidopsis thaliana. The Plant Cell. DOI:10.1105/tpc.20.00140 |
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