Nature Biotechnology
Abstract
Prime editors, which are CRISPR–Cas9 nickase (H840A)–reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.
| 论文编号: | DOI:10.1038/s41587-020-0455-x |
| 论文题目: | Prime Genome Editing in Rice and Wheat |
| 英文论文题目: | Prime Genome Editing in Rice and Wheat |
| 第一作者: | Qiupeng Lin, Yuan Zong, Chenxiao Xue, Shengxing Wang, Shuai Jin, Zixu Zhu, Yanpeng Wang, Andrew V. Anzalone, Aditya Raguram, Jordan L. Doman, David R. Liu & Caixia Gao |
| 英文第一作者: | Qiupeng Lin, Yuan Zong, Chenxiao Xue, Shengxing Wang, Shuai Jin, Zixu Zhu, Yanpeng Wang, Andrew V. Anzalone, Aditya Raguram, Jordan L. Doman, David R. Liu & Caixia Gao |
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| 发表年度: | 2020-03-17 |
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| 摘要: | Prime editors, which are CRISPR–Cas9 nickase (H840A)–reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%. |
| 英文摘要: | Prime editors, which are CRISPR–Cas9 nickase (H840A)–reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%. |
| 刊物名称: | Nature Biotechnology |
| 英文刊物名称: | Nature Biotechnology |
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| 其它备注: | Qiupeng Lin, Yuan Zong, Chenxiao Xue, Shengxing Wang, Shuai Jin, Zixu Zhu, Yanpeng Wang, Andrew V. Anzalone, Aditya Raguram, Jordan L. Doman, David R. Liu & Caixia Gao. Prime Genome Editing in Rice and Wheat. Nature Biotechnology. DOI:10.1038/s41587-020-0455-x |
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