Nature Communications
Abstract
Powdery mildew, caused by Blumeria graminis f. sp. tritici(Bgt), is one of the most destructive diseases that pose a great threat to wheat production. Wheat landraces represent a rich source of powdery mildew resistance.here, we report the map-based- cloning of powdery mildew resistance gene Pm24 from Chinese wheat landrace Hulutou It encodes a tandem kinase protein(tkp) with putative kinase-pseud- domains, designated WHEAT TANDEM KINASE(WTK3). The resistance function of Pm24 was validated by transgenic assay, independent mutants, and allelic association analyses. Haplotype analysis revealed that a rare 6-bp natural deletion of lysine-glycine codons, endemic to wheat landraces of Shaanxi Province, China, in the kinase I domain(Kin I) of WTK3 is critical for the resistance function. Transgenic assay of WTK3 chimeric variants revealed that only the specific two amino acid deletion, rather than any of the single or more amino acid deletions, in the Kin I of WTK3 is responsible for gaining the resistance function of WTK3 against the Bgt fungus.
| 论文编号: | DOI:10.1038/s41467-020-14294-0 |
| 论文题目: | A Rare Gain of Function Mutation in A Wheat Tandem Kinase Confers Resistance to Powdery Mildew |
| 英文论文题目: | A Rare Gain of Function Mutation in A Wheat Tandem Kinase Confers Resistance to Powdery Mildew |
| 第一作者: | Ping Lu, Li Guo, Zhenzhong Wang, Beibei Li, Jing Li, Yahui Li, Dan Qiu, Wengi Shi, Lijun Yang, Ning Wang, Guanghao Guo, Jingzhong Xie, Qiuhong Wu, Yongxing Chen, Miaomiao Li, Huaizhi Zhang, Lingli Dong, Panpan Zhang, Keyu Zhu, Dazhao Yu, Yan Zhang, Karin R. Deal, Naxin Huo, Cuimin Liu, Ming- Cheng Luo, Jan Dvorak, Yong Qiang Gu, Hongjie Li & Zhiyong Liu |
| 英文第一作者: | Ping Lu, Li Guo, Zhenzhong Wang, Beibei Li, Jing Li, Yahui Li, Dan Qiu, Wengi Shi, Lijun Yang, Ning Wang, Guanghao Guo, Jingzhong Xie, Qiuhong Wu, Yongxing Chen, Miaomiao Li, Huaizhi Zhang, Lingli Dong, Panpan Zhang, Keyu Zhu, Dazhao Yu, Yan Zhang, Karin R. Deal, Naxin Huo, Cuimin Liu, Ming- Cheng Luo, Jan Dvorak, Yong Qiang Gu, Hongjie Li & Zhiyong Liu |
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| 发表年度: | 2020-02-03 |
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| 摘要: | Powdery mildew, caused by Blumeria graminis f. sp. tritici(Bgt), is one of the most destructive diseases that pose a great threat to wheat production. Wheat landraces represent a rich source of powdery mildew resistance.here, we report the map-based- cloning of powdery mildew resistance gene Pm24 from Chinese wheat landrace Hulutou It encodes a tandem kinase protein(tkp) with putative kinase-pseud- domains, designated WHEAT TANDEM KINASE(WTK3). The resistance function of Pm24 was validated by transgenic assay, independent mutants, and allelic association analyses. Haplotype analysis revealed that a rare 6-bp natural deletion of lysine-glycine codons, endemic to wheat landraces of Shaanxi Province, China, in the kinase I domain(Kin I) of WTK3 is critical for the resistance function. Transgenic assay of WTK3 chimeric variants revealed that only the specific two amino acid deletion, rather than any of the single or more amino acid deletions, in the Kin I of WTK3 is responsible for gaining the resistance function of WTK3 against the Bgt fungus. |
| 英文摘要: | Powdery mildew, caused by Blumeria graminis f. sp. tritici(Bgt), is one of the most destructive diseases that pose a great threat to wheat production. Wheat landraces represent a rich source of powdery mildew resistance.here, we report the map-based- cloning of powdery mildew resistance gene Pm24 from Chinese wheat landrace Hulutou It encodes a tandem kinase protein(tkp) with putative kinase-pseud- domains, designated WHEAT TANDEM KINASE(WTK3). The resistance function of Pm24 was validated by transgenic assay, independent mutants, and allelic association analyses. Haplotype analysis revealed that a rare 6-bp natural deletion of lysine-glycine codons, endemic to wheat landraces of Shaanxi Province, China, in the kinase I domain(Kin I) of WTK3 is critical for the resistance function. Transgenic assay of WTK3 chimeric variants revealed that only the specific two amino acid deletion, rather than any of the single or more amino acid deletions, in the Kin I of WTK3 is responsible for gaining the resistance function of WTK3 against the Bgt fungus. |
| 刊物名称: | Nature Communications |
| 英文刊物名称: | Nature Communications |
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| 其它备注: | Ping Lu, Li Guo, Zhenzhong Wang, Beibei Li, Jing Li, Yahui Li, Dan Qiu, Wengi Shi, Lijun Yang, Ning Wang, Guanghao Guo, Jingzhong Xie, Qiuhong Wu, Yongxing Chen, Miaomiao Li, Huaizhi Zhang, Lingli Dong, Panpan Zhang, Keyu Zhu, Dazhao Yu, Yan Zhang, Karin R. Deal, Naxin Huo, Cuimin Liu, Ming- Cheng Luo, Jan Dvorak, Yong Qiang Gu, Hongjie Li & Zhiyong Liu. A Rare Gain of Function Mutation in A Wheat Tandem Kinase Confers Resistance to Powdery Mildew. Nature Communications. DOI:10.1038/s41467-020-14294-0 |
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