The Plant Journal
Abstract
Incorporating male sterility into hybrid seed production reduces its cost and ensureshighvarietalpurity. Despite these advantages, male‐sterile lines have not been widely used to produce tomato (Solanum lycopersicum) hybrid seeds. We describe the development of a biotechnology‐based breedingplatform that utilized genic male sterility to produce hybrid seeds. In this platform, we generated a novel male‐sterile tomato line by clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated protein 9 (Cas9)‐mediatedmutagenesis of a stamen‐specific geneSlSTR1and devised a transgenic maintainer by transforming male‐sterile plants with a fertility‐restoration gene linked to a seedling‐colour gene. Offspring of crosses between a hemizygous maintainer and the homozygous male‐sterile plant segregated into 50% non‐transgenic male‐sterile plants and 50% male‐fertile maintainer plants, which could be easily distinguished by seedling colour. This system has great practical potential for hybrid seed breeding and production as it overcomes the problems intrinsic to other male sterility systems and can be easily adapted for a range of tomato cultivars and diverse vegetable crops.
| 论文编号: | DOI:10.1111/tpj.14678 |
| 论文题目: | A Biotechnology‐Based Male Sterility System for Hybrid Seed Production in Tomato |
| 英文论文题目: | A Biotechnology‐Based Male Sterility System for Hybrid Seed Production in Tomato |
| 第一作者: | Minmin Du, Ke Zhou, Yuanyuan Liu, Lei Deng, Xiaoyue Zhang, Lihao Lin, Ming Zhou, Wei Zhao, Changlong Wen, Jiayi Xing, Chang-Bao Li, Chuanyou Li |
| 英文第一作者: | Minmin Du, Ke Zhou, Yuanyuan Liu, Lei Deng, Xiaoyue Zhang, Lihao Lin, Ming Zhou, Wei Zhao, Changlong Wen, Jiayi Xing, Chang-Bao Li, Chuanyou Li |
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| 发表年度: | 2020-01-13 |
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| 摘要: | Incorporating male sterility into hybrid seed production reduces its cost and ensureshighvarietalpurity. Despite these advantages, male‐sterile lines have not been widely used to produce tomato (Solanum lycopersicum) hybrid seeds. We describe the development of a biotechnology‐based breedingplatform that utilized genic male sterility to produce hybrid seeds. In this platform, we generated a novel male‐sterile tomato line by clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated protein 9 (Cas9)‐mediatedmutagenesis of a stamen‐specific geneSlSTR1and devised a transgenic maintainer by transforming male‐sterile plants with a fertility‐restoration gene linked to a seedling‐colour gene. Offspring of crosses between a hemizygous maintainer and the homozygous male‐sterile plant segregated into 50% non‐transgenic male‐sterile plants and 50% male‐fertile maintainer plants, which could be easily distinguished by seedling colour. This system has great practical potential for hybrid seed breeding and production as it overcomes the problems intrinsic to other male sterility systems and can be easily adapted for a range of tomato cultivars and diverse vegetable crops. |
| 英文摘要: | Incorporating male sterility into hybrid seed production reduces its cost and ensureshighvarietalpurity. Despite these advantages, male‐sterile lines have not been widely used to produce tomato (Solanum lycopersicum) hybrid seeds. We describe the development of a biotechnology‐based breedingplatform that utilized genic male sterility to produce hybrid seeds. In this platform, we generated a novel male‐sterile tomato line by clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated protein 9 (Cas9)‐mediatedmutagenesis of a stamen‐specific geneSlSTR1and devised a transgenic maintainer by transforming male‐sterile plants with a fertility‐restoration gene linked to a seedling‐colour gene. Offspring of crosses between a hemizygous maintainer and the homozygous male‐sterile plant segregated into 50% non‐transgenic male‐sterile plants and 50% male‐fertile maintainer plants, which could be easily distinguished by seedling colour. This system has great practical potential for hybrid seed breeding and production as it overcomes the problems intrinsic to other male sterility systems and can be easily adapted for a range of tomato cultivars and diverse vegetable crops. |
| 刊物名称: | The Plant Journal |
| 英文刊物名称: | The Plant Journal |
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| 其它备注: | Minmin Du, Ke Zhou, Yuanyuan Liu, Lei Deng, Xiaoyue Zhang, Lihao Lin, Ming Zhou, Wei Zhao, Changlong Wen, Jiayi Xing, Chang-Bao Li, Chuanyou Li. A Biotechnology‐Based Male Sterility System for Hybrid Seed Production in Tomato. The Plant Journal. DOI:10.1111/tpj.14678 |
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