PLoS Genetics
Abstract
Phosphatidylserine (PS), synthesized in the endoplasmic reticulum (ER) by phosphatidylserine synthetase (PSS), is transported to the plasma membrane (PM) and mitochondria through distinct routes. Thein vivofunctions of PS at different subcellular locations and the coordination between different PS transport routes are not fully understood. Here, we report thatDrosophilaPSS regulates cell growth, lipid storage and mitochondrial function. Inpss RNAi, reduced PS depletes plasma membrane Akt, contributing to cell growth defects; the metabolic shift from phospholipid synthesis to neutral lipid synthesis results in ectopic lipid accumulation; and the reduction of mitochondrial PS impairs mitochondrial protein import and mitochondrial integrity. Importantly, reducing PS transport from the ER to PM by loss ofPI4KIIIαpartially rescues the mitochondrial defects ofpss RNAi. Together, our results uncover a balance between different PS transport routes and reveal that PSS regulates cellular homeostasis through distinct metabolic mechanisms.
| 论文编号: | DOI:10.1371/journal.pgen.1008548 |
| 论文题目: | Phosphatidylserine Synthetase Regulates Cellular Homeostasis Through Distinct Metabolic Mechanisms |
| 英文论文题目: | Phosphatidylserine Synthetase Regulates Cellular Homeostasis Through Distinct Metabolic Mechanisms |
| 第一作者: | Xiao Yang, Jingjing Liang, Long Ding, Xia Li, Sin-Man Lam, Guanghou Shui, Mei Ding, Xun Huang |
| 英文第一作者: | Xiao Yang, Jingjing Liang, Long Ding, Xia Li, Sin-Man Lam, Guanghou Shui, Mei Ding, Xun Huang |
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| 发表年度: | 2019-12-26 |
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| 摘要: | Phosphatidylserine (PS), synthesized in the endoplasmic reticulum (ER) by phosphatidylserine synthetase (PSS), is transported to the plasma membrane (PM) and mitochondria through distinct routes. Thein vivofunctions of PS at different subcellular locations and the coordination between different PS transport routes are not fully understood. Here, we report thatDrosophilaPSS regulates cell growth, lipid storage and mitochondrial function. Inpss RNAi, reduced PS depletes plasma membrane Akt, contributing to cell growth defects; the metabolic shift from phospholipid synthesis to neutral lipid synthesis results in ectopic lipid accumulation; and the reduction of mitochondrial PS impairs mitochondrial protein import and mitochondrial integrity. Importantly, reducing PS transport from the ER to PM by loss ofPI4KIIIαpartially rescues the mitochondrial defects ofpss RNAi. Together, our results uncover a balance between different PS transport routes and reveal that PSS regulates cellular homeostasis through distinct metabolic mechanisms. |
| 英文摘要: | Phosphatidylserine (PS), synthesized in the endoplasmic reticulum (ER) by phosphatidylserine synthetase (PSS), is transported to the plasma membrane (PM) and mitochondria through distinct routes. Thein vivofunctions of PS at different subcellular locations and the coordination between different PS transport routes are not fully understood. Here, we report thatDrosophilaPSS regulates cell growth, lipid storage and mitochondrial function. Inpss RNAi, reduced PS depletes plasma membrane Akt, contributing to cell growth defects; the metabolic shift from phospholipid synthesis to neutral lipid synthesis results in ectopic lipid accumulation; and the reduction of mitochondrial PS impairs mitochondrial protein import and mitochondrial integrity. Importantly, reducing PS transport from the ER to PM by loss ofPI4KIIIαpartially rescues the mitochondrial defects ofpss RNAi. Together, our results uncover a balance between different PS transport routes and reveal that PSS regulates cellular homeostasis through distinct metabolic mechanisms. |
| 刊物名称: | PLoS Genetics |
| 英文刊物名称: | PLoS Genetics |
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| 其它备注: | Xiao Yang, Jingjing Liang, Long Ding, Xia Li, Sin-Man Lam, Guanghou Shui, Mei Ding, Xun Huang. Phosphatidylserine Synthetase Regulates Cellular Homeostasis Through Distinct Metabolic Mechanisms. PLoS Genetics. DOI:10.1371/journal.pgen.1008548 |
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