The Plant Cell
Abstract
JASMONATE ZIM-DOMAIN (JAZ) transcriptional repressors are key regulators of jasmonate (JA) signaling in plants. At the resting stage, the C-terminal Jas motifs of JAZ proteins bind the transcription factor MYC2 to repress JA signaling. Upon hormone elicitation, the Jas motif binds the hormone receptor COI1, which mediates proteasomal degradation of JAZs and thereby allowing the Mediator subunit MED25 to activate MYC2. Subsequently, plants desensitize JA signaling by feedback generation of dominant JAZ splice variants that repress MYC2. Here we report the mechanistic function of MED25 in regulating the alternative splicing of JAZ genes through recruiting the splicing factors PRE-mRNA-PROCESSING PROTEIN 39a (PRP39a) and PRP40a. We demonstrate that JA-induced generation of JAZ splice variants depends on MED25 and that MED25 recruits PRP39a and PRP40a to promote the full splicing of JAZ genes. Therefore, MED25 forms a module with PRP39a and PRP40a to prevent JAZ splice variants-mediated excessive desensitization of JA signaling.
| 论文编号: | DOI:10.1105/tpc.19.00583 |
| 论文题目: | Mediator Subunit MED25 Couples Alternative Splicing of JAZ Genes with Fine Tuning Jasmonate Signaling |
| 英文论文题目: | Mediator Subunit MED25 Couples Alternative Splicing of JAZ Genes with Fine Tuning Jasmonate Signaling |
| 第一作者: | Fangming Wu, Lei Deng, Qingzhe Zhai, Jiuhai Zhao, Qian Chen and Chuanyou Li |
| 英文第一作者: | Fangming Wu, Lei Deng, Qingzhe Zhai, Jiuhai Zhao, Qian Chen and Chuanyou Li |
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| 发表年度: | 2019-12-19 |
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| 摘要: | JASMONATE ZIM-DOMAIN (JAZ) transcriptional repressors are key regulators of jasmonate (JA) signaling in plants. At the resting stage, the C-terminal Jas motifs of JAZ proteins bind the transcription factor MYC2 to repress JA signaling. Upon hormone elicitation, the Jas motif binds the hormone receptor COI1, which mediates proteasomal degradation of JAZs and thereby allowing the Mediator subunit MED25 to activate MYC2. Subsequently, plants desensitize JA signaling by feedback generation of dominant JAZ splice variants that repress MYC2. Here we report the mechanistic function of MED25 in regulating the alternative splicing of JAZ genes through recruiting the splicing factors PRE-mRNA-PROCESSING PROTEIN 39a (PRP39a) and PRP40a. We demonstrate that JA-induced generation of JAZ splice variants depends on MED25 and that MED25 recruits PRP39a and PRP40a to promote the full splicing of JAZ genes. Therefore, MED25 forms a module with PRP39a and PRP40a to prevent JAZ splice variants-mediated excessive desensitization of JA signaling. |
| 英文摘要: | JASMONATE ZIM-DOMAIN (JAZ) transcriptional repressors are key regulators of jasmonate (JA) signaling in plants. At the resting stage, the C-terminal Jas motifs of JAZ proteins bind the transcription factor MYC2 to repress JA signaling. Upon hormone elicitation, the Jas motif binds the hormone receptor COI1, which mediates proteasomal degradation of JAZs and thereby allowing the Mediator subunit MED25 to activate MYC2. Subsequently, plants desensitize JA signaling by feedback generation of dominant JAZ splice variants that repress MYC2. Here we report the mechanistic function of MED25 in regulating the alternative splicing of JAZ genes through recruiting the splicing factors PRE-mRNA-PROCESSING PROTEIN 39a (PRP39a) and PRP40a. We demonstrate that JA-induced generation of JAZ splice variants depends on MED25 and that MED25 recruits PRP39a and PRP40a to promote the full splicing of JAZ genes. Therefore, MED25 forms a module with PRP39a and PRP40a to prevent JAZ splice variants-mediated excessive desensitization of JA signaling. |
| 刊物名称: | The Plant Cell |
| 英文刊物名称: | The Plant Cell |
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| 其它备注: | Fangming Wu, Lei Deng, Qingzhe Zhai, Jiuhai Zhao, Qian Chen and Chuanyou Li. Mediator Subunit MED25 Couples Alternative Splicing of JAZ Genes with Fine Tuning Jasmonate Signaling. The Plant Cell. DOI:10.1105/tpc.19.00583 |
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