The Plant Journal
Abstract
Structural Maintenance of Chromosomes 2 (SMC2) and Structural Maintenance of Chromosomes 4 (SMC4) are the core components of the condensin complexes, which are required for chromosome assembly and faithful segregation during cell division. Because of the crucial functions of both proteins in cell division, much work has been done in various vertebrates, but little is known about their roles in plants. Here, we identifiedZmSMC2andZmSMC4in maize (Zea mays) and confirmed that ZmSMC2 associates with ZmSMC4 via their hinge domains. Immunostaining revealed that both proteins showed dynamic localization during mitosis. ZmSMC2 and ZmSMC4 are essential for proper chromosome segregation and for H3 phosphorylation at Serine 10 (H3S10ph) at pericentromeres during mitotic division. The loss of function ofZmSMC2andZmSMC4enlarges mitotic chromosome volume and impairs sister chromatid separation to the opposite poles. Taken together, these findings confirm and extend the coordinated role of ZmSMC2 and ZmSMC4 in maintenance of normal chromosome architecture and accurate segregation during mitosis.
| 论文编号: | DOI:10.1111/tpj.14639 |
| 论文题目: | The Condensin Subunits SMC2 and SMC4 Interact for Correct Condensation and Segregation of Mitotic Maize Chromosomes |
| 英文论文题目: | The Condensin Subunits SMC2 and SMC4 Interact for Correct Condensation and Segregation of Mitotic Maize Chromosomes |
| 第一作者: | Hefei Wang, Yang Liu, Jing Yuan, Jing Zhang, Fangpu Han |
| 英文第一作者: | Hefei Wang, Yang Liu, Jing Yuan, Jing Zhang, Fangpu Han |
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| 发表年度: | 2019-12-11 |
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| 摘要: | Structural Maintenance of Chromosomes 2 (SMC2) and Structural Maintenance of Chromosomes 4 (SMC4) are the core components of the condensin complexes, which are required for chromosome assembly and faithful segregation during cell division. Because of the crucial functions of both proteins in cell division, much work has been done in various vertebrates, but little is known about their roles in plants. Here, we identifiedZmSMC2andZmSMC4in maize (Zea mays) and confirmed that ZmSMC2 associates with ZmSMC4 via their hinge domains. Immunostaining revealed that both proteins showed dynamic localization during mitosis. ZmSMC2 and ZmSMC4 are essential for proper chromosome segregation and for H3 phosphorylation at Serine 10 (H3S10ph) at pericentromeres during mitotic division. The loss of function ofZmSMC2andZmSMC4enlarges mitotic chromosome volume and impairs sister chromatid separation to the opposite poles. Taken together, these findings confirm and extend the coordinated role of ZmSMC2 and ZmSMC4 in maintenance of normal chromosome architecture and accurate segregation during mitosis. |
| 英文摘要: | Structural Maintenance of Chromosomes 2 (SMC2) and Structural Maintenance of Chromosomes 4 (SMC4) are the core components of the condensin complexes, which are required for chromosome assembly and faithful segregation during cell division. Because of the crucial functions of both proteins in cell division, much work has been done in various vertebrates, but little is known about their roles in plants. Here, we identifiedZmSMC2andZmSMC4in maize (Zea mays) and confirmed that ZmSMC2 associates with ZmSMC4 via their hinge domains. Immunostaining revealed that both proteins showed dynamic localization during mitosis. ZmSMC2 and ZmSMC4 are essential for proper chromosome segregation and for H3 phosphorylation at Serine 10 (H3S10ph) at pericentromeres during mitotic division. The loss of function ofZmSMC2andZmSMC4enlarges mitotic chromosome volume and impairs sister chromatid separation to the opposite poles. Taken together, these findings confirm and extend the coordinated role of ZmSMC2 and ZmSMC4 in maintenance of normal chromosome architecture and accurate segregation during mitosis. |
| 刊物名称: | The Plant Journal |
| 英文刊物名称: | The Plant Journal |
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| 其它备注: | Hefei Wang, Yang Liu, Jing Yuan, Jing Zhang, Fangpu Han. The Condensin Subunits SMC2 and SMC4 Interact for Correct Condensation and Segregation of Mitotic Maize Chromosomes. The Plant Journal. DOI:10.1111/tpj.14639 |
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