Nature Communications
Abstract
Message RNA poly(A) tails are vital for their function and regulation. However, the full-length sequence of mRNA isoforms with their poly(A) tails remains undetermined. Here, we develop a method at single-cell level sensitivity that enables quantification of poly(A) tails along with the full-length cDNA while reading non-adenosine residues within poly(A) tails precisely, which we name poly(A) inclusive RNA isoform sequencing (PAIso-seq). Using this method, we can quantify isoform specific poly(A) tail length. More interestingly, we find that 17% of the mRNAs harbor non-A residues within the body of poly(A) tails in mouse GV oocytes. We show that PAIso-seq is sensitive enough to analyze single GV oocytes. These findings will not only provide an accurate and sensitive tool in studying poly(A) tails, but also open a door for the function and regulation of non-adenosine modifications within the body of poly(A) tails.
| 论文编号: | DOI:10.1038/s41467-019-13228-9 |
| 论文题目: | Poly(A) Inclusive RNA Isoform Sequencing (PAIso-seq) Reveals Wide-spread Non-adenosine Residues Within RNA Poly(A) Tails |
| 英文论文题目: | Poly(A) Inclusive RNA Isoform Sequencing (PAIso-seq) Reveals Wide-spread Non-adenosine Residues Within RNA Poly(A) Tails |
| 第一作者: | Yusheng Liu, Hu Nie, Hongxiang Liu，Falong Lu |
| 英文第一作者: | Yusheng Liu, Hu Nie, Hongxiang Liu，Falong Lu |
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| 发表年度: | 2019-11-26 |
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| 摘要: | Message RNA poly(A) tails are vital for their function and regulation. However, the full-length sequence of mRNA isoforms with their poly(A) tails remains undetermined. Here, we develop a method at single-cell level sensitivity that enables quantification of poly(A) tails along with the full-length cDNA while reading non-adenosine residues within poly(A) tails precisely, which we name poly(A) inclusive RNA isoform sequencing (PAIso-seq). Using this method, we can quantify isoform specific poly(A) tail length. More interestingly, we find that 17% of the mRNAs harbor non-A residues within the body of poly(A) tails in mouse GV oocytes. We show that PAIso-seq is sensitive enough to analyze single GV oocytes. These findings will not only provide an accurate and sensitive tool in studying poly(A) tails, but also open a door for the function and regulation of non-adenosine modifications within the body of poly(A) tails. |
| 英文摘要: | Message RNA poly(A) tails are vital for their function and regulation. However, the full-length sequence of mRNA isoforms with their poly(A) tails remains undetermined. Here, we develop a method at single-cell level sensitivity that enables quantification of poly(A) tails along with the full-length cDNA while reading non-adenosine residues within poly(A) tails precisely, which we name poly(A) inclusive RNA isoform sequencing (PAIso-seq). Using this method, we can quantify isoform specific poly(A) tail length. More interestingly, we find that 17% of the mRNAs harbor non-A residues within the body of poly(A) tails in mouse GV oocytes. We show that PAIso-seq is sensitive enough to analyze single GV oocytes. These findings will not only provide an accurate and sensitive tool in studying poly(A) tails, but also open a door for the function and regulation of non-adenosine modifications within the body of poly(A) tails. |
| 刊物名称: | Nature Communications |
| 英文刊物名称: | Nature Communications |
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| 其它备注: | Yusheng Liu, Hu Nie, Hongxiang Liu，Falong Lu. Poly(A) Inclusive RNA Isoform Sequencing (PAIso-seq) Reveals Wide-spread Non-adenosine Residues Within RNA Poly(A) Tails. Nature Communications. DOI:10.1038/s41467-019-13228-9 |
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