The Plant Journal
Abstract
Pre‐harvest sprouting (PHS) is one of the major problems in cereal production worldwide, which causes significant loss of both yield and quality. However, the molecular mechanism underlying is still largely unknown. Here, we identified a dominant pre‐harvest sprouting mutantphs9‐D. The corresponding genePHS9encodes a higher plant unique CC‐type glutaredoxin and is specifically expressed in embryo at the late embryogenesis stage, implying thatPHS9plays some roles in late stage of seed development. Yeast two hybrid screening showed that PHS9 could interact with OsGAP, which is an interaction partner of the ABA receptor OsRCAR1.PHS9orOsGAPoverexpression plants showed reduced, whilePHS9orOsGAPknockout mutant plants showed increased ABA sensitivity in seed germination, suggesting that PHS9 and OsGAP acted as negative regulators in ABA signaling during seed germination. Interestingly, the germination ofPHS9andOsGAPoverexpression or knockout plant seeds was weakly promoted by H2O2, implying that PHS9 and OsGAP could affect the ROS signaling during seed germination. These results indicates that PHS9 plays an important role in the regulation of rice PHS through integration of ROS signaling and ABA signaling.
| 论文编号: | DOI:10.1111/tpj.14501 |
| 论文题目: | Control of Rice Pre‐harvest Sprouting by Glutaredoxin‐mediated Abscisic Acid Signaling |
| 英文论文题目: | Control of Rice Pre‐harvest Sprouting by Glutaredoxin‐mediated Abscisic Acid Signaling |
| 第一作者: | Fan Xu, Jiuyou Tang, Shaopei Gao, Xi Cheng, Lin Du, Chengcai Chu |
| 英文第一作者: | Fan Xu, Jiuyou Tang, Shaopei Gao, Xi Cheng, Lin Du, Chengcai Chu |
| 联系作者: | |
| 英文联系作者: | |
| 外单位作者单位: | |
| 英文外单位作者单位: | |
| 发表年度: | 2019-08-23 |
| 卷: | |
| 期: | |
| 页码: | |
| 摘要: | Pre‐harvest sprouting (PHS) is one of the major problems in cereal production worldwide, which causes significant loss of both yield and quality. However, the molecular mechanism underlying is still largely unknown. Here, we identified a dominant pre‐harvest sprouting mutantphs9‐D. The corresponding genePHS9encodes a higher plant unique CC‐type glutaredoxin and is specifically expressed in embryo at the late embryogenesis stage, implying thatPHS9plays some roles in late stage of seed development. Yeast two hybrid screening showed that PHS9 could interact with OsGAP, which is an interaction partner of the ABA receptor OsRCAR1.PHS9orOsGAPoverexpression plants showed reduced, whilePHS9orOsGAPknockout mutant plants showed increased ABA sensitivity in seed germination, suggesting that PHS9 and OsGAP acted as negative regulators in ABA signaling during seed germination. Interestingly, the germination ofPHS9andOsGAPoverexpression or knockout plant seeds was weakly promoted by H2O2, implying that PHS9 and OsGAP could affect the ROS signaling during seed germination. These results indicates that PHS9 plays an important role in the regulation of rice PHS through integration of ROS signaling and ABA signaling. |
| 英文摘要: | Pre‐harvest sprouting (PHS) is one of the major problems in cereal production worldwide, which causes significant loss of both yield and quality. However, the molecular mechanism underlying is still largely unknown. Here, we identified a dominant pre‐harvest sprouting mutantphs9‐D. The corresponding genePHS9encodes a higher plant unique CC‐type glutaredoxin and is specifically expressed in embryo at the late embryogenesis stage, implying thatPHS9plays some roles in late stage of seed development. Yeast two hybrid screening showed that PHS9 could interact with OsGAP, which is an interaction partner of the ABA receptor OsRCAR1.PHS9orOsGAPoverexpression plants showed reduced, whilePHS9orOsGAPknockout mutant plants showed increased ABA sensitivity in seed germination, suggesting that PHS9 and OsGAP acted as negative regulators in ABA signaling during seed germination. Interestingly, the germination ofPHS9andOsGAPoverexpression or knockout plant seeds was weakly promoted by H2O2, implying that PHS9 and OsGAP could affect the ROS signaling during seed germination. These results indicates that PHS9 plays an important role in the regulation of rice PHS through integration of ROS signaling and ABA signaling. |
| 刊物名称: | The Plant Journal |
| 英文刊物名称: | The Plant Journal |
| 论文全文: | |
| 英文论文全文: | |
| 全文链接: | |
| 其它备注: | Fan Xu, Jiuyou Tang, Shaopei Gao, Xi Cheng, Lin Du, Chengcai Chu. Control of Rice Pre‐harvest Sprouting by Glutaredoxin‐mediated Abscisic Acid Signaling. The Plant Journal. DOI:10.1111/tpj.14501 |
| 英文其它备注: | |
| 学科: | |
| 英文学科: | |
| 影响因子: | |
| 第一作者所在部门: | |
| 英文第一作者所在部门: | |
| 论文出处: | |
| 英文论文出处: | |
| 论文类别: | |
| 英文论文类别: | |
| 参与作者: | |
| 英文参与作者: |
