Plant Physiology
Abstract
During growth, plant cells must coordinate cell expansion and cell wall reinforcement by integrating distinct regulatory pathways in concert with intrinsic and external cues. However, the mechanism underpinning this integration is unclear, as few of the regulators that orchestrate cell expansion and wall strengthening have been identified. Here, we report a rice (Oryza sativa) Class II KNOX-like homeobox protein KNOTTED ARABIDOPSIS THALIANA7 interacts with different partners to govern cell expansion and wall thickening. A loss-of-function mutation in KNAT7 enhanced wall mechanical strength and cell expansion, resulting in improved lodging resistance and grain size. Overexpression of KNAT7 gave rise to the opposite phenotypes, with plants having weaker cell walls and smaller grains. Biochemical and gene expression analyses revealed that rice KNAT7 interacts with a secondary wall key regulator, NAC31, and a cell growth master regulator, GROWTH-REGULATING FACTOR 4 (GRF4). The KNAT7-NAC31 and KNAT7-GRF4 modules suppressed regulatory pathways of cell expansion and wall reinforcement, as we show in internode and panicle development. These modules function in sclerenchyma fiber cells and modulate fiber cell length and wall thickness. Hence, our study uncovers a mechanism for the combined control of cell size and wall strengthening, providing a tool to improve lodging resistance and yield in rice production.
| 论文编号: | DOI:10.1104/pp.19.00639 |
| 论文题目: | A Rice Homeobox Protein KNAT7 Integrates the Pathways Regulating Cell Expansion And Wall Stiffness |
| 英文论文题目: | A Rice Homeobox Protein KNAT7 Integrates the Pathways Regulating Cell Expansion And Wall Stiffness |
| 第一作者: | Shaogan Wang, Hanlei Yang, Jiasong Mei, Xiangling Liu, Zhao Wen, Lanjun Zhang, Zuopeng Xu, Baocai Zhang and Yihua Zhou |
| 英文第一作者: | Shaogan Wang, Hanlei Yang, Jiasong Mei, Xiangling Liu, Zhao Wen, Lanjun Zhang, Zuopeng Xu, Baocai Zhang and Yihua Zhou |
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| 发表年度: | 2019-08-02 |
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| 摘要: | During growth, plant cells must coordinate cell expansion and cell wall reinforcement by integrating distinct regulatory pathways in concert with intrinsic and external cues. However, the mechanism underpinning this integration is unclear, as few of the regulators that orchestrate cell expansion and wall strengthening have been identified. Here, we report a rice (Oryza sativa) Class II KNOX-like homeobox protein KNOTTED ARABIDOPSIS THALIANA7 interacts with different partners to govern cell expansion and wall thickening. A loss-of-function mutation in KNAT7 enhanced wall mechanical strength and cell expansion, resulting in improved lodging resistance and grain size. Overexpression of KNAT7 gave rise to the opposite phenotypes, with plants having weaker cell walls and smaller grains. Biochemical and gene expression analyses revealed that rice KNAT7 interacts with a secondary wall key regulator, NAC31, and a cell growth master regulator, GROWTH-REGULATING FACTOR 4 (GRF4). The KNAT7-NAC31 and KNAT7-GRF4 modules suppressed regulatory pathways of cell expansion and wall reinforcement, as we show in internode and panicle development. These modules function in sclerenchyma fiber cells and modulate fiber cell length and wall thickness. Hence, our study uncovers a mechanism for the combined control of cell size and wall strengthening, providing a tool to improve lodging resistance and yield in rice production. |
| 英文摘要: | During growth, plant cells must coordinate cell expansion and cell wall reinforcement by integrating distinct regulatory pathways in concert with intrinsic and external cues. However, the mechanism underpinning this integration is unclear, as few of the regulators that orchestrate cell expansion and wall strengthening have been identified. Here, we report a rice (Oryza sativa) Class II KNOX-like homeobox protein KNOTTED ARABIDOPSIS THALIANA7 interacts with different partners to govern cell expansion and wall thickening. A loss-of-function mutation in KNAT7 enhanced wall mechanical strength and cell expansion, resulting in improved lodging resistance and grain size. Overexpression of KNAT7 gave rise to the opposite phenotypes, with plants having weaker cell walls and smaller grains. Biochemical and gene expression analyses revealed that rice KNAT7 interacts with a secondary wall key regulator, NAC31, and a cell growth master regulator, GROWTH-REGULATING FACTOR 4 (GRF4). The KNAT7-NAC31 and KNAT7-GRF4 modules suppressed regulatory pathways of cell expansion and wall reinforcement, as we show in internode and panicle development. These modules function in sclerenchyma fiber cells and modulate fiber cell length and wall thickness. Hence, our study uncovers a mechanism for the combined control of cell size and wall strengthening, providing a tool to improve lodging resistance and yield in rice production. |
| 刊物名称: | Plant Physiology |
| 英文刊物名称: | Plant Physiology |
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| 其它备注: | Shaogan Wang, Hanlei Yang, Jiasong Mei, Xiangling Liu, Zhao Wen, Lanjun Zhang, Zuopeng Xu, Baocai Zhang and Yihua Zhou. A Rice Homeobox Protein KNAT7 Integrates the Pathways Regulating Cell Expansion And Wall Stiffness. Plant Physiology. DOI:10.1104/pp.19.00639 |
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