Nature Plants
Abstract
The lipid-derived hormone jasmonate (JA) regulates plant immunity and adaptive growth by triggering a genome-wide transcriptional programme. In Arabidopsis thaliana, JA-triggered transcriptional programming is largely orchestrated by the master transcription factor MYC2. The function of MYC2 is dependent on its physical interaction with the MED25 subunit of the Mediator transcriptional co-activator complex. Here we report the identification of JA enhancers (JAEs) through profiling the occupancy pattern of MYC2 and MED25. JA regulates the dynamic chromatin looping between JAEs and their promoters in a MED25-dependent manner, while MYC2 auto-regulates itself through JAEs. Interestingly, the JAE of the MYC2 locus (named ME2) positively regulates MYC2 expression during short-term JA responses but negatively regulates it during constant JA responses. We demonstrate that new gene editing tools open up new avenues to elucidate the in vivo function of enhancers.
| 论文编号: | DOI:10.1038/s41477-019-0441-9 |
| 论文题目: | MED25 Connects Enhancer-Promoter Looping and MYC2-Dependent Activation of Jasmonate Signaling |
| 英文论文题目: | MED25 Connects Enhancer-Promoter Looping and MYC2-Dependent Activation of Jasmonate Signaling |
| 第一作者: | Hang Wang, Shuyu Li, Yan’an Li, Yiran Xu, Yunhao Wang, Ruoxi Zhang, Wenjing Sun, Qian Chen, Xiu-jie Wang, Chuanyou Li, Jiuhai Zhao |
| 英文第一作者: | Hang Wang, Shuyu Li, Yan’an Li, Yiran Xu, Yunhao Wang, Ruoxi Zhang, Wenjing Sun, Qian Chen, Xiu-jie Wang, Chuanyou Li, Jiuhai Zhao |
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| 发表年度: | 2019-06-12 |
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| 摘要: | The lipid-derived hormone jasmonate (JA) regulates plant immunity and adaptive growth by triggering a genome-wide transcriptional programme. In Arabidopsis thaliana, JA-triggered transcriptional programming is largely orchestrated by the master transcription factor MYC2. The function of MYC2 is dependent on its physical interaction with the MED25 subunit of the Mediator transcriptional co-activator complex. Here we report the identification of JA enhancers (JAEs) through profiling the occupancy pattern of MYC2 and MED25. JA regulates the dynamic chromatin looping between JAEs and their promoters in a MED25-dependent manner, while MYC2 auto-regulates itself through JAEs. Interestingly, the JAE of the MYC2 locus (named ME2) positively regulates MYC2 expression during short-term JA responses but negatively regulates it during constant JA responses. We demonstrate that new gene editing tools open up new avenues to elucidate the in vivo function of enhancers. |
| 英文摘要: | The lipid-derived hormone jasmonate (JA) regulates plant immunity and adaptive growth by triggering a genome-wide transcriptional programme. In Arabidopsis thaliana, JA-triggered transcriptional programming is largely orchestrated by the master transcription factor MYC2. The function of MYC2 is dependent on its physical interaction with the MED25 subunit of the Mediator transcriptional co-activator complex. Here we report the identification of JA enhancers (JAEs) through profiling the occupancy pattern of MYC2 and MED25. JA regulates the dynamic chromatin looping between JAEs and their promoters in a MED25-dependent manner, while MYC2 auto-regulates itself through JAEs. Interestingly, the JAE of the MYC2 locus (named ME2) positively regulates MYC2 expression during short-term JA responses but negatively regulates it during constant JA responses. We demonstrate that new gene editing tools open up new avenues to elucidate the in vivo function of enhancers. |
| 刊物名称: | Nature Plants |
| 英文刊物名称: | Nature Plants |
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| 其它备注: | Hang Wang, Shuyu Li, Yan’an Li, Yiran Xu, Yunhao Wang, Ruoxi Zhang, Wenjing Sun, Qian Chen, Xiu-jie Wang, Chuanyou Li, Jiuhai Zhao. MED25 Connects Enhancer-Promoter Looping and MYC2-Dependent Activation of Jasmonate Signaling. Nature Plants. DOI:10.1038/s41477-019-0441-9 |
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