Science
Abstract
Pathogen recognition by nucleotide-binding (NB), leucine-rich repeat (LRR) receptors (NLRs) plays roles in plant immunity. The Xanthomonas campestris pv. campestris effector AvrAC uridylylates the Arabidopsis PBL2 kinase, and the latter (PBL2UMP) acts as a ligand to activate the NLR ZAR1 precomplexed with the RKS1 pseudokinase. Here we report the cryo–electron microscopy structures of ZAR1-RKS1 and ZAR1-RKS1-PBL2UMP in an inactive and intermediate state, respectively. The ZAR1LRR domain, compared with animal NLRLRR domains, is differently positioned to sequester ZAR1 in an inactive state. Recognition of PBL2UMP is exclusively through RKS1, which interacts with ZAR1LRR. PBL2UMP binding stabilizes the RKS1 activation segment, which sterically blocks ZAR1 adenosine diphosphate (ADP) binding. This engenders a more flexible NB domain without conformational changes in the other ZAR1 domains. Our study provides a structural template for understanding plant NLRs.
论文编号: | DOI:10.1126/science.aav5868 |
论文题目: | Ligand-triggered Allosteric ADP Release Primes a Plant NLR Complex |
英文论文题目: | Ligand-triggered Allosteric ADP Release Primes a Plant NLR Complex |
第一作者: | Jizong Wang, Jia Wang, Meijuan Hu, Shan Wu, Jinfeng Qi, Guoxun Wang, Zhifu Han, Yijun Qi, Ning Gao, Hong-Wei Wang, Jian-Min Zhou, Jijie Chai |
英文第一作者: | Jizong Wang, Jia Wang, Meijuan Hu, Shan Wu, Jinfeng Qi, Guoxun Wang, Zhifu Han, Yijun Qi, Ning Gao, Hong-Wei Wang, Jian-Min Zhou, Jijie Chai |
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发表年度: | 2019-04-05 |
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摘要: | Pathogen recognition by nucleotide-binding (NB), leucine-rich repeat (LRR) receptors (NLRs) plays roles in plant immunity. The Xanthomonas campestris pv. campestris effector AvrAC uridylylates the Arabidopsis PBL2 kinase, and the latter (PBL2UMP) acts as a ligand to activate the NLR ZAR1 precomplexed with the RKS1 pseudokinase. Here we report the cryo–electron microscopy structures of ZAR1-RKS1 and ZAR1-RKS1-PBL2UMP in an inactive and intermediate state, respectively. The ZAR1LRR domain, compared with animal NLRLRR domains, is differently positioned to sequester ZAR1 in an inactive state. Recognition of PBL2UMP is exclusively through RKS1, which interacts with ZAR1LRR. PBL2UMP binding stabilizes the RKS1 activation segment, which sterically blocks ZAR1 adenosine diphosphate (ADP) binding. This engenders a more flexible NB domain without conformational changes in the other ZAR1 domains. Our study provides a structural template for understanding plant NLRs. |
英文摘要: | Pathogen recognition by nucleotide-binding (NB), leucine-rich repeat (LRR) receptors (NLRs) plays roles in plant immunity. The Xanthomonas campestris pv. campestris effector AvrAC uridylylates the Arabidopsis PBL2 kinase, and the latter (PBL2UMP) acts as a ligand to activate the NLR ZAR1 precomplexed with the RKS1 pseudokinase. Here we report the cryo–electron microscopy structures of ZAR1-RKS1 and ZAR1-RKS1-PBL2UMP in an inactive and intermediate state, respectively. The ZAR1LRR domain, compared with animal NLRLRR domains, is differently positioned to sequester ZAR1 in an inactive state. Recognition of PBL2UMP is exclusively through RKS1, which interacts with ZAR1LRR. PBL2UMP binding stabilizes the RKS1 activation segment, which sterically blocks ZAR1 adenosine diphosphate (ADP) binding. This engenders a more flexible NB domain without conformational changes in the other ZAR1 domains. Our study provides a structural template for understanding plant NLRs. |
刊物名称: | Science |
英文刊物名称: | Science |
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其它备注: | Jizong Wang, Jia Wang, Meijuan Hu, Shan Wu, Jinfeng Qi, Guoxun Wang, Zhifu Han, Yijun Qi, Ning Gao, Hong-Wei Wang, Jian-Min Zhou, Jijie Chai. Ligand-triggered Allosteric ADP Release Primes a Plant NLR Complex. Science. DOI:10.1126/science.aav5868 |
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