Nature Communications
Abstract
Gene regulatory networks control development via domain-specific gene expression. In seed plants, self-renewing stem cells located in the shoot apical meristem (SAM) produce leaves from the SAM peripheral zone. After initiation, leaves develop polarity patterns to form a planar shape. Here we compare translating RNAs among SAM and leaf domains. Using translating ribosome affinity purification and RNA sequencing to quantify gene expression in target domains, we generate a domain-specific translatome map covering representative vegetative stage SAM and leaf domains. We discuss the predicted cellular functions of these domains and provide evidence that dome seemingly unrelated domains, utilize common regulatory modules. Experimental follow up shows that theRABBIT EARSandHANABA TARANUtranscription factors have roles in axillary meristem initiation. This dataset provides a community resource for further study of shoot development and response to internal and environmental signals.
论文编号: | DOI:10.1038/s41467-018-08083-z |
论文题目: | A Gene Expression Map of Shoot Domains Reveals Regulatory Mechanisms |
英文论文题目: | A Gene Expression Map of Shoot Domains Reveals Regulatory Mechanisms |
第一作者: | Caihuan Tian, Ying Wang, Haopeng Yu, Jun He, Jin Wang, Bihai Shi, Qingwei Du, Nicholas J. Provart, Elliot M. Meyerowitz & Yuling Jiao |
英文第一作者: | Caihuan Tian, Ying Wang, Haopeng Yu, Jun He, Jin Wang, Bihai Shi, Qingwei Du, Nicholas J. Provart, Elliot M. Meyerowitz & Yuling Jiao |
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发表年度: | 2019-01-14 |
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摘要: | Gene regulatory networks control development via domain-specific gene expression. In seed plants, self-renewing stem cells located in the shoot apical meristem (SAM) produce leaves from the SAM peripheral zone. After initiation, leaves develop polarity patterns to form a planar shape. Here we compare translating RNAs among SAM and leaf domains. Using translating ribosome affinity purification and RNA sequencing to quantify gene expression in target domains, we generate a domain-specific translatome map covering representative vegetative stage SAM and leaf domains. We discuss the predicted cellular functions of these domains and provide evidence that dome seemingly unrelated domains, utilize common regulatory modules. Experimental follow up shows that theRABBIT EARSandHANABA TARANUtranscription factors have roles in axillary meristem initiation. This dataset provides a community resource for further study of shoot development and response to internal and environmental signals. |
英文摘要: | Gene regulatory networks control development via domain-specific gene expression. In seed plants, self-renewing stem cells located in the shoot apical meristem (SAM) produce leaves from the SAM peripheral zone. After initiation, leaves develop polarity patterns to form a planar shape. Here we compare translating RNAs among SAM and leaf domains. Using translating ribosome affinity purification and RNA sequencing to quantify gene expression in target domains, we generate a domain-specific translatome map covering representative vegetative stage SAM and leaf domains. We discuss the predicted cellular functions of these domains and provide evidence that dome seemingly unrelated domains, utilize common regulatory modules. Experimental follow up shows that theRABBIT EARSandHANABA TARANUtranscription factors have roles in axillary meristem initiation. This dataset provides a community resource for further study of shoot development and response to internal and environmental signals. |
刊物名称: | Nature Communications |
英文刊物名称: | Nature Communications |
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其它备注: | Caihuan Tian, Ying Wang, Haopeng Yu, Jun He, Jin Wang, Bihai Shi, Qingwei Du, Nicholas J. Provart, Elliot M. Meyerowitz & Yuling Jiao. A Gene Expression Map of Shoot Domains Reveals Regulatory Mechanisms. Nature Communications. DOI:10.1038/s41467-018-08083-z |
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