Nature Biotechnology
Abstract
Heterosis, or hybrid vigor, is exploited by breeders to produce elite high-yielding crop lines, but beneficial phenotypes are lost in subsequent generations owing to genetic segregation. Clonal propagation through seeds would enable self-propagation of F1hybrids. Here we report a strategy to enable clonal reproduction of F1rice hybrids through seeds. We fixed the heterozygosity of F1hybrid rice by multiplex CRISPR–Cas9 genome editing of theREC8,PAIR1andOSD1meiotic genes to produce clonal diploid gametes and tetraploid seeds. Next, we demonstrated that editing theMATRILINEAL(MTL) gene (involved in fertilization) could induce formation of haploid seeds in hybrid rice. Finally, we combined fixation of heterozygosity and haploid induction by simultaneous editing of all four genes (REC8,PAIR1,OSD1andMTL)in hybrid rice and obtained plants that could propagate clonally through seeds. Application of our method may enable self-propagation of a broad range of elite F1hybrid crops.
| 论文编号: | DOI:10.1038/s41587-018-0003-0 |
| 论文题目: | Clonal Seeds from Hybrid Rice by Simultaneous Genome Engineering of Meiosis and Fertilization Genes |
| 英文论文题目: | Clonal Seeds from Hybrid Rice by Simultaneous Genome Engineering of Meiosis and Fertilization Genes |
| 第一作者: | Chun Wang, Qing Liu, Yi Shen, Yufeng Hua, Junjie Wang, Jianrong Lin, Mingguo Wu, Tingting Sun, Zhukuan Cheng, Raphael Mercier & Kejian Wang |
| 英文第一作者: | Chun Wang, Qing Liu, Yi Shen, Yufeng Hua, Junjie Wang, Jianrong Lin, Mingguo Wu, Tingting Sun, Zhukuan Cheng, Raphael Mercier & Kejian Wang |
| 联系作者: | |
| 英文联系作者: | |
| 外单位作者单位: | |
| 英文外单位作者单位: | |
| 发表年度: | 2019-01-07 |
| 卷: | |
| 期: | |
| 页码: | |
| 摘要: | Heterosis, or hybrid vigor, is exploited by breeders to produce elite high-yielding crop lines, but beneficial phenotypes are lost in subsequent generations owing to genetic segregation. Clonal propagation through seeds would enable self-propagation of F1hybrids. Here we report a strategy to enable clonal reproduction of F1rice hybrids through seeds. We fixed the heterozygosity of F1hybrid rice by multiplex CRISPR–Cas9 genome editing of theREC8,PAIR1andOSD1meiotic genes to produce clonal diploid gametes and tetraploid seeds. Next, we demonstrated that editing theMATRILINEAL(MTL) gene (involved in fertilization) could induce formation of haploid seeds in hybrid rice. Finally, we combined fixation of heterozygosity and haploid induction by simultaneous editing of all four genes (REC8,PAIR1,OSD1andMTL)in hybrid rice and obtained plants that could propagate clonally through seeds. Application of our method may enable self-propagation of a broad range of elite F1hybrid crops. |
| 英文摘要: | Heterosis, or hybrid vigor, is exploited by breeders to produce elite high-yielding crop lines, but beneficial phenotypes are lost in subsequent generations owing to genetic segregation. Clonal propagation through seeds would enable self-propagation of F1hybrids. Here we report a strategy to enable clonal reproduction of F1rice hybrids through seeds. We fixed the heterozygosity of F1hybrid rice by multiplex CRISPR–Cas9 genome editing of theREC8,PAIR1andOSD1meiotic genes to produce clonal diploid gametes and tetraploid seeds. Next, we demonstrated that editing theMATRILINEAL(MTL) gene (involved in fertilization) could induce formation of haploid seeds in hybrid rice. Finally, we combined fixation of heterozygosity and haploid induction by simultaneous editing of all four genes (REC8,PAIR1,OSD1andMTL)in hybrid rice and obtained plants that could propagate clonally through seeds. Application of our method may enable self-propagation of a broad range of elite F1hybrid crops. |
| 刊物名称: | Nature Biotechnology |
| 英文刊物名称: | Nature Biotechnology |
| 论文全文: | |
| 英文论文全文: | |
| 全文链接: | |
| 其它备注: | Chun Wang, Qing Liu, Yi Shen, Yufeng Hua, Junjie Wang, Jianrong Lin, Mingguo Wu, Tingting Sun, Zhukuan Cheng, Raphael Mercier & Kejian Wang. Clonal Seeds from Hybrid Rice by Simultaneous Genome Engineering of Meiosis and Fertilization Genes. Nature Biotechnology. DOI:10.1038/s41587-018-0003-0 |
| 英文其它备注: | |
| 学科: | |
| 英文学科: | |
| 影响因子: | |
| 第一作者所在部门: | |
| 英文第一作者所在部门: | |
| 论文出处: | |
| 英文论文出处: | |
| 论文类别: | |
| 英文论文类别: | |
| 参与作者: | |
| 英文参与作者: |
