The Plant Cell
Abstract
In tomato (Solanum lycopersicum), as with in other plants, the immunity hormone jasmonate (JA) triggers genome-wide transcriptional changes in response to pathogen and insect attack. These changes are largely regulated by the basic helix-loop-helix (bHLH) transcription factor MYC2. The function of MYC2 depends on its physical interaction with the MED25 subunit of the Mediator transcriptional co-activator complex. Although much has been learned about the MYC2-dependent transcriptional activation of JA-responsive genes, relatively less studied is the termination of JA-mediated transcriptional responses and the underlying mechanisms. Here, we report an unexpected function of MYC2 in regulating the termination of JA signaling through activating a small group of JA-inducible bHLH proteins, termed MYC2-TARGETED BHLH 1 (MTB1), MTB2, and MTB3. MTB proteins negatively regulate JA-mediated transcriptional responses via their antagonistic effects on the functionality of the MYC2-MED25 transcriptional activation complex. MTB proteins impair the formation of the MYC2-MED25 complex and compete with MYC2 to bind to its target gene promoters. Therefore, MYC2 and MTB proteins form an autoregulatory negative feedback circuit to terminate JA signaling in a highly organized manner. We exemplify that new gene editing tools, such as CRISPR/Cas9, open up new avenues to exploit MTB genes for crop protection.
| 论文编号: | DOI:10.1105/tpc.18.00405 |
| 论文题目: | MYC2 Regulates the Termination of Jasmonate Signaling via an Autoregulatory Negative Feedback Loop |
| 英文论文题目: | MYC2 Regulates the Termination of Jasmonate Signaling via an Autoregulatory Negative Feedback Loop |
| 第一作者: | Yuanyuan Liu, Minmin Du, Lei Deng, Jiafang Shen, Mingming Fang, Qian Chen, Yanhui Lu, Qiaomei Wang, Chuanyou Li, and Qingzhe Zhai |
| 英文第一作者: | Yuanyuan Liu, Minmin Du, Lei Deng, Jiafang Shen, Mingming Fang, Qian Chen, Yanhui Lu, Qiaomei Wang, Chuanyou Li, and Qingzhe Zhai |
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| 发表年度: | 2019-01-07 |
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| 摘要: | In tomato (Solanum lycopersicum), as with in other plants, the immunity hormone jasmonate (JA) triggers genome-wide transcriptional changes in response to pathogen and insect attack. These changes are largely regulated by the basic helix-loop-helix (bHLH) transcription factor MYC2. The function of MYC2 depends on its physical interaction with the MED25 subunit of the Mediator transcriptional co-activator complex. Although much has been learned about the MYC2-dependent transcriptional activation of JA-responsive genes, relatively less studied is the termination of JA-mediated transcriptional responses and the underlying mechanisms. Here, we report an unexpected function of MYC2 in regulating the termination of JA signaling through activating a small group of JA-inducible bHLH proteins, termed MYC2-TARGETED BHLH 1 (MTB1), MTB2, and MTB3. MTB proteins negatively regulate JA-mediated transcriptional responses via their antagonistic effects on the functionality of the MYC2-MED25 transcriptional activation complex. MTB proteins impair the formation of the MYC2-MED25 complex and compete with MYC2 to bind to its target gene promoters. Therefore, MYC2 and MTB proteins form an autoregulatory negative feedback circuit to terminate JA signaling in a highly organized manner. We exemplify that new gene editing tools, such as CRISPR/Cas9, open up new avenues to exploit MTB genes for crop protection. |
| 英文摘要: | In tomato (Solanum lycopersicum), as with in other plants, the immunity hormone jasmonate (JA) triggers genome-wide transcriptional changes in response to pathogen and insect attack. These changes are largely regulated by the basic helix-loop-helix (bHLH) transcription factor MYC2. The function of MYC2 depends on its physical interaction with the MED25 subunit of the Mediator transcriptional co-activator complex. Although much has been learned about the MYC2-dependent transcriptional activation of JA-responsive genes, relatively less studied is the termination of JA-mediated transcriptional responses and the underlying mechanisms. Here, we report an unexpected function of MYC2 in regulating the termination of JA signaling through activating a small group of JA-inducible bHLH proteins, termed MYC2-TARGETED BHLH 1 (MTB1), MTB2, and MTB3. MTB proteins negatively regulate JA-mediated transcriptional responses via their antagonistic effects on the functionality of the MYC2-MED25 transcriptional activation complex. MTB proteins impair the formation of the MYC2-MED25 complex and compete with MYC2 to bind to its target gene promoters. Therefore, MYC2 and MTB proteins form an autoregulatory negative feedback circuit to terminate JA signaling in a highly organized manner. We exemplify that new gene editing tools, such as CRISPR/Cas9, open up new avenues to exploit MTB genes for crop protection. |
| 刊物名称: | The Plant Cell |
| 英文刊物名称: | The Plant Cell |
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| 其它备注: | Yuanyuan Liu, Minmin Du, Lei Deng, Jiafang Shen, Mingming Fang, Qian Chen, Yanhui Lu, Qiaomei Wang, Chuanyou Li, and Qingzhe Zhai. MYC2 Regulates the Termination of Jasmonate Signaling via an Autoregulatory Negative Feedback Loop. The Plant Cell. DOI:10.1105/tpc.18.00405 |
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