New Phytologist
Abstract
Meiotic recombination is closely linked with homologous pairing and synapsis. Previous studies have shown that HOMOLOGOUS PAIRING PROTEIN2 (Hop2), plays an essential role in homologous pairing and synapsis. However, the mechanism by which HOP2 regulates crossover (CO) formation has not been elucidated. Here, we show that OsHOP2 mediates the maturation of COs by promoting homologous pairing and synapsis in rice meiosis.
We used a combination of genetic analysis, immunolocalization and super‐resolution imaging to analyze the function of OsHOP2 in rice meiosis.
We showed that full‐length pairing, synapsis and CO formation are disturbed in Oshop2 meiocytes. Moreover, structured illumination microscopy (SIM) showed that OsHOP2 localized to chromatin and displayed considerable co‐localization with axial elements (AEs) and central elements (CEs). Importantly, the interaction between OsHOP2 and ZEP1, provided further evidence that OsHOP2 was involved in assembly or stabilization of the structure of the synaptonemal complex (SC). Although the initiation of recombination and CO designation occur normally in Oshop2 mutants, mature COs were severely reduced, and HEI10 foci were only present on the synapsed region.
Together, we speculate that OsHOP2 may serve as a global regulator to coordinate homologous pairing, synapsis and meiotic recombination in rice meiosis.
| 论文编号: | DOI:10.1111/nph.15664 |
| 论文题目: | OsHOP2 Regulates the Maturation of Crossovers by Promoting Homologous Pairing and Synapsis in Rice Meiosis |
| 英文论文题目: | OsHOP2 Regulates the Maturation of Crossovers by Promoting Homologous Pairing and Synapsis in Rice Meiosis |
| 第一作者: | Wenqing Shi, Ding Tang, Yi Shen, Zhihui Xue, Fanfan Zhang, Chao Zhang, Lijun Ren, Changzhen Liu, Guijie Du, Yafei Li, Changjie Yan, Zhukuan Cheng |
| 英文第一作者: | Wenqing Shi, Ding Tang, Yi Shen, Zhihui Xue, Fanfan Zhang, Chao Zhang, Lijun Ren, Changzhen Liu, Guijie Du, Yafei Li, Changjie Yan, Zhukuan Cheng |
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| 发表年度: | 2018-12-26 |
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| 摘要: | Meiotic recombination is closely linked with homologous pairing and synapsis. Previous studies have shown that HOMOLOGOUS PAIRING PROTEIN2 (Hop2), plays an essential role in homologous pairing and synapsis. However, the mechanism by which HOP2 regulates crossover (CO) formation has not been elucidated. Here, we show that OsHOP2 mediates the maturation of COs by promoting homologous pairing and synapsis in rice meiosis. We used a combination of genetic analysis, immunolocalization and super‐resolution imaging to analyze the function of OsHOP2 in rice meiosis. We showed that full‐length pairing, synapsis and CO formation are disturbed in Oshop2 meiocytes. Moreover, structured illumination microscopy (SIM) showed that OsHOP2 localized to chromatin and displayed considerable co‐localization with axial elements (AEs) and central elements (CEs). Importantly, the interaction between OsHOP2 and ZEP1, provided further evidence that OsHOP2 was involved in assembly or stabilization of the structure of the synaptonemal complex (SC). Although the initiation of recombination and CO designation occur normally in Oshop2 mutants, mature COs were severely reduced, and HEI10 foci were only present on the synapsed region. Together, we speculate that OsHOP2 may serve as a global regulator to coordinate homologous pairing, synapsis and meiotic recombination in rice meiosis. |
| 英文摘要: | Meiotic recombination is closely linked with homologous pairing and synapsis. Previous studies have shown that HOMOLOGOUS PAIRING PROTEIN2 (Hop2), plays an essential role in homologous pairing and synapsis. However, the mechanism by which HOP2 regulates crossover (CO) formation has not been elucidated. Here, we show that OsHOP2 mediates the maturation of COs by promoting homologous pairing and synapsis in rice meiosis. We used a combination of genetic analysis, immunolocalization and super‐resolution imaging to analyze the function of OsHOP2 in rice meiosis. We showed that full‐length pairing, synapsis and CO formation are disturbed in Oshop2 meiocytes. Moreover, structured illumination microscopy (SIM) showed that OsHOP2 localized to chromatin and displayed considerable co‐localization with axial elements (AEs) and central elements (CEs). Importantly, the interaction between OsHOP2 and ZEP1, provided further evidence that OsHOP2 was involved in assembly or stabilization of the structure of the synaptonemal complex (SC). Although the initiation of recombination and CO designation occur normally in Oshop2 mutants, mature COs were severely reduced, and HEI10 foci were only present on the synapsed region. Together, we speculate that OsHOP2 may serve as a global regulator to coordinate homologous pairing, synapsis and meiotic recombination in rice meiosis. |
| 刊物名称: | New Phytologist |
| 英文刊物名称: | New Phytologist |
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| 其它备注: | Wenqing Shi, Ding Tang, Yi Shen, Zhihui Xue, Fanfan Zhang, Chao Zhang, Lijun Ren, Changzhen Liu, Guijie Du, Yafei Li, Changjie Yan, Zhukuan Cheng. OsHOP2 Regulates the Maturation of Crossovers by Promoting Homologous Pairing and Synapsis in Rice Meiosis. New Phytologist. DOI:10.1111/nph.15664. |
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