Plant Biotechnology Journal
Abstract
CRISPR-Cas9 systems have been widely used in animals and plants for genome editing, epigenetic modification, etc. (Cong et al., 2013, Hilton et al., 2015). However, the accurate recognition of target sites of CRISPR/Cas9 systems depends on the single-guide RNA (sgRNA) corresponding to each target site and protospacer adjacent motifs (PAMs) around these sites (Anders et al., 2014). Cas9-sgRNA complexes will directly eject the DNA template if no PAMs are recognized before base pairing between target sites and sgRNA (Sternberg et al., 2014).
| 论文编号: | DOI:10.1111/pbi.13053 |
| 论文题目: | xCas9 Expands the Scope of Genome Editing with Reduced Efficiency in Rice |
| 英文论文题目: | xCas9 Expands the Scope of Genome Editing with Reduced Efficiency in Rice |
| 第一作者: | Junjie Wang, Xiangbing Meng, Xixun Hu, Tingting Sun, Jiayang Li, Kejian Wang, Hong Yu |
| 英文第一作者: | Junjie Wang, Xiangbing Meng, Xixun Hu, Tingting Sun, Jiayang Li, Kejian Wang, Hong Yu |
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| 发表年度: | 2018-12-25 |
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| 摘要: | CRISPR-Cas9 systems have been widely used in animals and plants for genome editing, epigenetic modification, etc. (Cong et al., 2013, Hilton et al., 2015). However, the accurate recognition of target sites of CRISPR/Cas9 systems depends on the single-guide RNA (sgRNA) corresponding to each target site and protospacer adjacent motifs (PAMs) around these sites (Anders et al., 2014). Cas9-sgRNA complexes will directly eject the DNA template if no PAMs are recognized before base pairing between target sites and sgRNA (Sternberg et al., 2014). |
| 英文摘要: | CRISPR-Cas9 systems have been widely used in animals and plants for genome editing, epigenetic modification, etc. (Cong et al., 2013, Hilton et al., 2015). However, the accurate recognition of target sites of CRISPR/Cas9 systems depends on the single-guide RNA (sgRNA) corresponding to each target site and protospacer adjacent motifs (PAMs) around these sites (Anders et al., 2014). Cas9-sgRNA complexes will directly eject the DNA template if no PAMs are recognized before base pairing between target sites and sgRNA (Sternberg et al., 2014). |
| 刊物名称: | Plant Biotechnology Journal |
| 英文刊物名称: | Plant Biotechnology Journal |
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| 其它备注: | Junjie Wang, Xiangbing Meng, Xixun Hu, Tingting Sun, Jiayang Li, Kejian Wang, Hong Yu. xCas9 Expands the Scope of Genome Editing with Reduced Efficiency in Rice. Plant Biotechnology Journal. DOI:10.1111/pbi.13053 |
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