Plant Biotechnology Journal
Abstract
Selenium (Se) is an essential trace element for humans and other animals, yet approximately one billion people worldwide suffer from Se deficiency. Rice is a staple food for over half of the world's population that is a major dietary source of Se. In paddy soils, rice roots mainly take up selenite. Se speciation analysis indicated that most of the selenite absorbed by rice is predominantly transformed into selenomethinone (SeMet) and retained in roots. However, the mechanism by which SeMet is transported in plants remains largely unknown. In this study, SeMet uptake was found to be an energy‐dependent symport process involving H+transport, with neutral amino acids strongly inhibiting SeMet uptake. We further revealed that NRT1.1B, a member of rice peptide transporter (PTR) family which plays an important role in nitrate uptake and transport in rice, displays SeMet transport activity in yeast andXenopusoocyte. The uptake rate of SeMet in the roots and its accumulation rate in the shoots ofnrt1.1bmutant were significantly repressed. Conversely, the overexpression ofNRT1.1Bin rice significantly promoted SeMet translocation from roots to shoots, resulting in increased Se concentrations in shoots and rice grains. With vascular‐specific expression ofNRT1.1B, the grain Se concentration was 1.83‐fold higher than that of wild type. These results strongly demonstrate that NRT1.1B holds great potential for the improvement of Se concentrations in grains by facilitating SeMet translocation, and the findings provide novel insight into breeding of Se‐enriched rice varieties.
论文编号: | DOI:10.1111/pbi.13037 |
论文题目: | NRT1.1B Improves Selenium Concentrations in Rice Grains by Facilitating Selenomethinone Translocation |
英文论文题目: | NRT1.1B Improves Selenium Concentrations in Rice Grains by Facilitating Selenomethinone Translocation |
第一作者: | Lianhe Zhang, Bin Hu, Kun Deng, Xiaokai Gao, Guoxin Sun, Zhengli Zhang, Peng Li, Wei Wang, Hua Li, Zhihua Zhang, Zihao Fu, Jinyong Yang, Shaopei Gao, Legong Li, Feiyan Yu, Youjun Li, Hongqing Ling, Chengcai Chu |
英文第一作者: | Lianhe Zhang, Bin Hu, Kun Deng, Xiaokai Gao, Guoxin Sun, Zhengli Zhang, Peng Li, Wei Wang, Hua Li, Zhihua Zhang, Zihao Fu, Jinyong Yang, Shaopei Gao, Legong Li, Feiyan Yu, Youjun Li, Hongqing Ling, Chengcai Chu |
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发表年度: | 2018-11-27 |
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摘要: | Selenium (Se) is an essential trace element for humans and other animals, yet approximately one billion people worldwide suffer from Se deficiency. Rice is a staple food for over half of the world's population that is a major dietary source of Se. In paddy soils, rice roots mainly take up selenite. Se speciation analysis indicated that most of the selenite absorbed by rice is predominantly transformed into selenomethinone (SeMet) and retained in roots. However, the mechanism by which SeMet is transported in plants remains largely unknown. In this study, SeMet uptake was found to be an energy‐dependent symport process involving H+transport, with neutral amino acids strongly inhibiting SeMet uptake. We further revealed that NRT1.1B, a member of rice peptide transporter (PTR) family which plays an important role in nitrate uptake and transport in rice, displays SeMet transport activity in yeast andXenopusoocyte. The uptake rate of SeMet in the roots and its accumulation rate in the shoots ofnrt1.1bmutant were significantly repressed. Conversely, the overexpression ofNRT1.1Bin rice significantly promoted SeMet translocation from roots to shoots, resulting in increased Se concentrations in shoots and rice grains. With vascular‐specific expression ofNRT1.1B, the grain Se concentration was 1.83‐fold higher than that of wild type. These results strongly demonstrate that NRT1.1B holds great potential for the improvement of Se concentrations in grains by facilitating SeMet translocation, and the findings provide novel insight into breeding of Se‐enriched rice varieties. |
英文摘要: | Selenium (Se) is an essential trace element for humans and other animals, yet approximately one billion people worldwide suffer from Se deficiency. Rice is a staple food for over half of the world's population that is a major dietary source of Se. In paddy soils, rice roots mainly take up selenite. Se speciation analysis indicated that most of the selenite absorbed by rice is predominantly transformed into selenomethinone (SeMet) and retained in roots. However, the mechanism by which SeMet is transported in plants remains largely unknown. In this study, SeMet uptake was found to be an energy‐dependent symport process involving H+transport, with neutral amino acids strongly inhibiting SeMet uptake. We further revealed that NRT1.1B, a member of rice peptide transporter (PTR) family which plays an important role in nitrate uptake and transport in rice, displays SeMet transport activity in yeast andXenopusoocyte. The uptake rate of SeMet in the roots and its accumulation rate in the shoots ofnrt1.1bmutant were significantly repressed. Conversely, the overexpression ofNRT1.1Bin rice significantly promoted SeMet translocation from roots to shoots, resulting in increased Se concentrations in shoots and rice grains. With vascular‐specific expression ofNRT1.1B, the grain Se concentration was 1.83‐fold higher than that of wild type. These results strongly demonstrate that NRT1.1B holds great potential for the improvement of Se concentrations in grains by facilitating SeMet translocation, and the findings provide novel insight into breeding of Se‐enriched rice varieties. |
刊物名称: | Plant Biotechnology Journal |
英文刊物名称: | Plant Biotechnology Journal |
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其它备注: | Lianhe Zhang, Bin Hu, Kun Deng, Xiaokai Gao, Guoxin Sun, Zhengli Zhang, Peng Li, Wei Wang, Hua Li, Zhihua Zhang, Zihao Fu, Jinyong Yang, Shaopei Gao, Legong Li, Feiyan Yu, Youjun Li, Hongqing Ling, Chengcai Chu. NRT1.1B Improves Selenium Concentrations in Rice Grains by Facilitating Selenomethinone Translocation. Plant Biotechnology Journal. DOI:10.1111/pbi.13037 |
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