PLoS Genetics
Abstract
Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility,defective callose in meiosis 1(dcm1). The pollen grains ofdcm1are proved to be defective in exine formation. Meiotic cytokinesis is disrupted indcm1, resulting in disordered spindle orientation during meiosis II and formation of pollen grains with varied size and DNA content. We demonstrated that meiotic cytokinesis defect indcm1is caused by prematurely dissolution of callosic plates. Furthermore, peripheral callose surrounding thedcm1pollen mother cells (PMCs) also disappeared untimely around pachytene. The DCM1 protein contains five tandem CCCH motifs and interacts with nuclear poly (A) binding proteins (PABNs) in nuclear speckles. The expression profiles of genes related to callose synthesis and degradation are significantly modified indcm1. Together, we propose that DCM1 plays an essential role in male meiotic cytokinesis by preserving callose from prematurely dissolution in rice.
| 论文编号: | DOI:10.1371/journal.pgen.1007769 |
| 论文题目: | The Zinc Finger Protein DCM1 is Required for Male Meiotic Cytokinesis by Preserving Callose in Rice |
| 英文论文题目: | The Zinc Finger Protein DCM1 is Required for Male Meiotic Cytokinesis by Preserving Callose in Rice |
| 第一作者: | Chao Zhang, Yi Shen, Ding Tang, Wenqing Shi, Dongmei Zhang, Guijie Du, Yihua Zhou, Guohua Liang, Yafei Li , Zhukuan Cheng |
| 英文第一作者: | Chao Zhang, Yi Shen, Ding Tang, Wenqing Shi, Dongmei Zhang, Guijie Du, Yihua Zhou, Guohua Liang, Yafei Li , Zhukuan Cheng |
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| 发表年度: | 2018-11-13 |
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| 摘要: | Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility,defective callose in meiosis 1(dcm1). The pollen grains ofdcm1are proved to be defective in exine formation. Meiotic cytokinesis is disrupted indcm1, resulting in disordered spindle orientation during meiosis II and formation of pollen grains with varied size and DNA content. We demonstrated that meiotic cytokinesis defect indcm1is caused by prematurely dissolution of callosic plates. Furthermore, peripheral callose surrounding thedcm1pollen mother cells (PMCs) also disappeared untimely around pachytene. The DCM1 protein contains five tandem CCCH motifs and interacts with nuclear poly (A) binding proteins (PABNs) in nuclear speckles. The expression profiles of genes related to callose synthesis and degradation are significantly modified indcm1. Together, we propose that DCM1 plays an essential role in male meiotic cytokinesis by preserving callose from prematurely dissolution in rice. |
| 英文摘要: | Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility,defective callose in meiosis 1(dcm1). The pollen grains ofdcm1are proved to be defective in exine formation. Meiotic cytokinesis is disrupted indcm1, resulting in disordered spindle orientation during meiosis II and formation of pollen grains with varied size and DNA content. We demonstrated that meiotic cytokinesis defect indcm1is caused by prematurely dissolution of callosic plates. Furthermore, peripheral callose surrounding thedcm1pollen mother cells (PMCs) also disappeared untimely around pachytene. The DCM1 protein contains five tandem CCCH motifs and interacts with nuclear poly (A) binding proteins (PABNs) in nuclear speckles. The expression profiles of genes related to callose synthesis and degradation are significantly modified indcm1. Together, we propose that DCM1 plays an essential role in male meiotic cytokinesis by preserving callose from prematurely dissolution in rice. |
| 刊物名称: | PLoS Genetics |
| 英文刊物名称: | PLoS Genetics |
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| 其它备注: | Chao Zhang, Yi Shen, Ding Tang, Wenqing Shi, Dongmei Zhang, Guijie Du, Yihua Zhou, Guohua Liang, Yafei Li , Zhukuan Cheng. The Zinc Finger Protein DCM1 is Required for Male Meiotic Cytokinesis by Preserving Callose in Rice. PLoS Genetics. DOI:10.1371/journal.pgen.1007769 |
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