Cell Reports
Abstract
Lipid droplets (LDs) are highly dynamic organelles that store neutral lipids. Through a gene overexpression screen in the Drosophila larval fat body, we have identified that MRT, an Myb/switching-defective protein 3 (Swi3), Adaptor 2 (Ada2), Nuclear receptor co-repressor (N-CoR), Transcription factor (TF)IIIB (SANT)-like DNA-binding domain-containing protein, regulates LD size and lipid storage. MRT directly interacts with, and is functionally dependent on, the PZG and NURF chromatin-remodeling complex components. MRT binds to the promoter of plin1, the gene encoding the LD-resident protein perilipin, and inhibits the transcription of plin1. In vitro LD coalescence assays suggest that mrt overexpression or loss of plin1 function facilitates LD coalescence. Our findings suggest that MRT functions together with chromatin-remodeling factors to regulate LD size, likely through the transcriptional repression of plin1.
| 论文编号: | DOI:10.1016/j.celrep.2018.08.026 |
| 论文题目: | MRT, Functioning with NURF Complex, Regulates Lipid Droplet Size |
| 英文论文题目: | MRT, Functioning with NURF Complex, Regulates Lipid Droplet Size |
| 第一作者: | Yan Yao, Xia Li, Wei Wang, Zhonghua Liu, Jianming Chen, Mei Ding & Xun Huang |
| 英文第一作者: | Yan Yao, Xia Li, Wei Wang, Zhonghua Liu, Jianming Chen, Mei Ding & Xun Huang |
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| 发表年度: | 2018-09-12 |
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| 摘要: | Lipid droplets (LDs) are highly dynamic organelles that store neutral lipids. Through a gene overexpression screen in the Drosophila larval fat body, we have identified that MRT, an Myb/switching-defective protein 3 (Swi3), Adaptor 2 (Ada2), Nuclear receptor co-repressor (N-CoR), Transcription factor (TF)IIIB (SANT)-like DNA-binding domain-containing protein, regulates LD size and lipid storage. MRT directly interacts with, and is functionally dependent on, the PZG and NURF chromatin-remodeling complex components. MRT binds to the promoter of plin1, the gene encoding the LD-resident protein perilipin, and inhibits the transcription of plin1. In vitro LD coalescence assays suggest that mrt overexpression or loss of plin1 function facilitates LD coalescence. Our findings suggest that MRT functions together with chromatin-remodeling factors to regulate LD size, likely through the transcriptional repression of plin1. |
| 英文摘要: | Lipid droplets (LDs) are highly dynamic organelles that store neutral lipids. Through a gene overexpression screen in the Drosophila larval fat body, we have identified that MRT, an Myb/switching-defective protein 3 (Swi3), Adaptor 2 (Ada2), Nuclear receptor co-repressor (N-CoR), Transcription factor (TF)IIIB (SANT)-like DNA-binding domain-containing protein, regulates LD size and lipid storage. MRT directly interacts with, and is functionally dependent on, the PZG and NURF chromatin-remodeling complex components. MRT binds to the promoter of plin1, the gene encoding the LD-resident protein perilipin, and inhibits the transcription of plin1. In vitro LD coalescence assays suggest that mrt overexpression or loss of plin1 function facilitates LD coalescence. Our findings suggest that MRT functions together with chromatin-remodeling factors to regulate LD size, likely through the transcriptional repression of plin1. |
| 刊物名称: | Cell Reports |
| 英文刊物名称: | Cell Reports |
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| 其它备注: | Yan Yao, Xia Li, Wei Wang, Zhonghua Liu, Jianming Chen, Mei Ding & Xun Huang. MRT, Functioning with NURF Complex, Regulates Lipid Droplet Size. Cell Reports. DOI:10.1016/j.celrep.2018.08.026 |
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