Nature Biotechnology
Abstract
Translational regulation by upstream open reading frames (uORFs) is becoming established as a general mechanism for controlling the amount of protein that is synthesized from downstream primary ORFs (pORFs). We found that genome editing of endogenous uORFs in plants enabled the modulation of translation of mRNAs from four pORFs that are involved in either development or antioxidant biosynthesis. A single-guide RNA that targeted the region harboring a uORF initiation codon can produce multiple mutations. Following uORF editing, we observed varying amounts of mRNA translation in four pORFs. Notably, editing the uORF of LsGGP2, which encodes a key enzyme in vitamin C biosynthesis in lettuce, not only increased oxidation stress tolerance, but also increased ascorbate content by ~150%. These data indicate that editing plant uORFs provides a generalizable, efficient method for manipulating translation of mRNA that could be applied to dissect biological mechanisms and improve crops.
论文编号: | DOI:10.1038/nbt.4202 |
论文题目: | Genome Editing of Upstream Open Reading Frames Enables Translational Control in Plants |
英文论文题目: | Genome Editing of Upstream Open Reading Frames Enables Translational Control in Plants |
第一作者: | Huawei Zhang, Xiaomin Si, Xiang Ji, Rong Fan, Jinxing Liu, Kunling Chen, Daowen Wang & Caixia Gao |
英文第一作者: | Huawei Zhang, Xiaomin Si, Xiang Ji, Rong Fan, Jinxing Liu, Kunling Chen, Daowen Wang & Caixia Gao |
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发表年度: | 2018-08-07 |
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摘要: | Translational regulation by upstream open reading frames (uORFs) is becoming established as a general mechanism for controlling the amount of protein that is synthesized from downstream primary ORFs (pORFs). We found that genome editing of endogenous uORFs in plants enabled the modulation of translation of mRNAs from four pORFs that are involved in either development or antioxidant biosynthesis. A single-guide RNA that targeted the region harboring a uORF initiation codon can produce multiple mutations. Following uORF editing, we observed varying amounts of mRNA translation in four pORFs. Notably, editing the uORF of LsGGP2, which encodes a key enzyme in vitamin C biosynthesis in lettuce, not only increased oxidation stress tolerance, but also increased ascorbate content by ~150%. These data indicate that editing plant uORFs provides a generalizable, efficient method for manipulating translation of mRNA that could be applied to dissect biological mechanisms and improve crops. |
英文摘要: | Translational regulation by upstream open reading frames (uORFs) is becoming established as a general mechanism for controlling the amount of protein that is synthesized from downstream primary ORFs (pORFs). We found that genome editing of endogenous uORFs in plants enabled the modulation of translation of mRNAs from four pORFs that are involved in either development or antioxidant biosynthesis. A single-guide RNA that targeted the region harboring a uORF initiation codon can produce multiple mutations. Following uORF editing, we observed varying amounts of mRNA translation in four pORFs. Notably, editing the uORF of LsGGP2, which encodes a key enzyme in vitamin C biosynthesis in lettuce, not only increased oxidation stress tolerance, but also increased ascorbate content by ~150%. These data indicate that editing plant uORFs provides a generalizable, efficient method for manipulating translation of mRNA that could be applied to dissect biological mechanisms and improve crops. |
刊物名称: | Nature Biotechnology |
英文刊物名称: | Nature Biotechnology |
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其它备注: | Huawei Zhang, Xiaomin Si, Xiang Ji, Rong Fan, Jinxing Liu, Kunling Chen, Daowen Wang & Caixia Gao. Genome Editing of Upstream Open Reading Frames Enables Translational Control in Plants. Nature Biotechnology. DOI:10.1038/nbt.4202 |
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