Molecular Cell
Abstract
Nitric oxide (NO) regulates diverse cellular signaling through S-nitrosylation of specific Cys residues of target proteins. The intracellular level of S-nitrosoglutathione (GSNO), a major bioactive NO species, is regulated by GSNO reductase (GSNOR), a highly conserved master regulator of NO signaling. However, little is known about how the activity of GSNOR is regulated. Here, we show that S-nitrosylation induces selective autophagy of Arabidopsis GSNOR1 during hypoxia responses. S-nitrosylation of GSNOR1 at Cys-10 induces conformational changes, exposing its AUTOPHAGY-RELATED8 (ATG8)-interacting motif (AIM) accessible by autophagy machinery. Upon binding by ATG8, GSNOR1 is recruited into the autophagosome and degraded in an AIM-dependent manner. Physiologically, the S-nitrosylation-induced selective autophagy of GSNOR1 is relevant to hypoxia responses. Our discovery reveals a unique mechanism by which S-nitrosylation mediates selective autophagy of GSNOR1, thereby establishing a molecular link between NO signaling and autophagy.
| 论文编号: | DOI:10.1016/j.molcel.2018.05.024 |
| 论文题目: | S-Nitrosylation Targets GSNO Reductase for Selective Autophagy during Hypoxia Responses in Plants |
| 英文论文题目: | S-Nitrosylation Targets GSNO Reductase for Selective Autophagy during Hypoxia Responses in Plants |
| 第一作者: | Ni Zhan, Chun Wang, Lichao Chen, Huanjie Yang, Jian Feng, Xinqi Gong, Bo Ren, Rong Wu, Jinye Mu, Yansha Li, Zhonghua Liu, Ying Zhou, Juli Peng, Kejian Wang, Xun Huang, Shi Xiao, Jianru Zuo |
| 英文第一作者: | Ni Zhan, Chun Wang, Lichao Chen, Huanjie Yang, Jian Feng, Xinqi Gong, Bo Ren, Rong Wu, Jinye Mu, Yansha Li, Zhonghua Liu, Ying Zhou, Juli Peng, Kejian Wang, Xun Huang, Shi Xiao, Jianru Zuo |
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| 发表年度: | 2018-06-29 |
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| 摘要: | Nitric oxide (NO) regulates diverse cellular signaling through S-nitrosylation of specific Cys residues of target proteins. The intracellular level of S-nitrosoglutathione (GSNO), a major bioactive NO species, is regulated by GSNO reductase (GSNOR), a highly conserved master regulator of NO signaling. However, little is known about how the activity of GSNOR is regulated. Here, we show that S-nitrosylation induces selective autophagy of Arabidopsis GSNOR1 during hypoxia responses. S-nitrosylation of GSNOR1 at Cys-10 induces conformational changes, exposing its AUTOPHAGY-RELATED8 (ATG8)-interacting motif (AIM) accessible by autophagy machinery. Upon binding by ATG8, GSNOR1 is recruited into the autophagosome and degraded in an AIM-dependent manner. Physiologically, the S-nitrosylation-induced selective autophagy of GSNOR1 is relevant to hypoxia responses. Our discovery reveals a unique mechanism by which S-nitrosylation mediates selective autophagy of GSNOR1, thereby establishing a molecular link between NO signaling and autophagy. |
| 英文摘要: | Nitric oxide (NO) regulates diverse cellular signaling through S-nitrosylation of specific Cys residues of target proteins. The intracellular level of S-nitrosoglutathione (GSNO), a major bioactive NO species, is regulated by GSNO reductase (GSNOR), a highly conserved master regulator of NO signaling. However, little is known about how the activity of GSNOR is regulated. Here, we show that S-nitrosylation induces selective autophagy of Arabidopsis GSNOR1 during hypoxia responses. S-nitrosylation of GSNOR1 at Cys-10 induces conformational changes, exposing its AUTOPHAGY-RELATED8 (ATG8)-interacting motif (AIM) accessible by autophagy machinery. Upon binding by ATG8, GSNOR1 is recruited into the autophagosome and degraded in an AIM-dependent manner. Physiologically, the S-nitrosylation-induced selective autophagy of GSNOR1 is relevant to hypoxia responses. Our discovery reveals a unique mechanism by which S-nitrosylation mediates selective autophagy of GSNOR1, thereby establishing a molecular link between NO signaling and autophagy. |
| 刊物名称: | Molecular Cell |
| 英文刊物名称: | Molecular Cell |
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| 其它备注: | Ni Zhan, Chun Wang, Lichao Chen, Huanjie Yang, Jian Feng, Xinqi Gong, Bo Ren, Rong Wu, Jinye Mu, Yansha Li, Zhonghua Liu, Ying Zhou, Juli Peng, Kejian Wang, Xun Huang, Shi Xiao, Jianru Zuo. S-Nitrosylation Targets GSNO Reductase for Selective Autophagy during Hypoxia Responses in Plants. Molecular Cell. DOI:10.1016/j.molcel.2018.05.024 |
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