The Plant Journal
Abstract
WUSCHEL(WUS) is critical for plant meristem maintenance and determinacy in Arabidopsis, and the regulation of its spatiotemporal expression patterns is complex. We previously found that AGAMOUS (AG), a key MADS‐domain transcription factor in floral organ identity and floral meristem determinacy, can directly suppressWUS expression through the recruitment of the Polycomb group (PcG) protein TERMINAL FLOWER2 (TFL2, also known as LIKE HETEROCHROMATIN PROTEIN1, LHP1) at theWUS locus; however, the mechanism by whichWUSis repressed remains unclear. Here, using chromosome conformation capture (3C) and chromatin immunoprecipitation 3C, we found that two specific regions flanking theWUSgene body bound by AG and TFL2 form a chromatin loop that is directly promoted by AG during flower development in a manner independent of the physical distance and sequence content of the intervening region. Moreover, AG physically interacts with TFL2, and TFL2 binding to the chromatin loop is dependent on AG. Transgenic and CRISPR/Cas9‐edited lines showed that theWUSchromatin loop represses gene expression by blocking the recruitment of RNA polymerase II at the locus. The findings uncover theWUSchromatin loop as another regulatory mechanism controllingWUSexpression, and also shed light on the factors required for chromatin conformation change and their recruitment.
| 论文编号: | DOI:10.1111/tpj.13921 |
| 论文题目: | A Chromatin Loop Represses WUSCHEL Expression in Arabidopsis |
| 英文论文题目: | A Chromatin Loop Represses WUSCHEL Expression in Arabidopsis |
| 第一作者: | Lin Guo, Xiuwei Cao, Yuhao Liu, Jun Li, Yongpeng Li, Dongming Li, Ke Zhang, Caixia Gao, Aiwu Dong, Xigang Liu |
| 英文第一作者: | Lin Guo, Xiuwei Cao, Yuhao Liu, Jun Li, Yongpeng Li, Dongming Li, Ke Zhang, Caixia Gao, Aiwu Dong, Xigang Liu |
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| 发表年度: | 2018-06-12 |
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| 摘要: | WUSCHEL(WUS) is critical for plant meristem maintenance and determinacy in Arabidopsis, and the regulation of its spatiotemporal expression patterns is complex. We previously found that AGAMOUS (AG), a key MADS‐domain transcription factor in floral organ identity and floral meristem determinacy, can directly suppressWUS expression through the recruitment of the Polycomb group (PcG) protein TERMINAL FLOWER2 (TFL2, also known as LIKE HETEROCHROMATIN PROTEIN1, LHP1) at theWUS locus; however, the mechanism by whichWUSis repressed remains unclear. Here, using chromosome conformation capture (3C) and chromatin immunoprecipitation 3C, we found that two specific regions flanking theWUSgene body bound by AG and TFL2 form a chromatin loop that is directly promoted by AG during flower development in a manner independent of the physical distance and sequence content of the intervening region. Moreover, AG physically interacts with TFL2, and TFL2 binding to the chromatin loop is dependent on AG. Transgenic and CRISPR/Cas9‐edited lines showed that theWUSchromatin loop represses gene expression by blocking the recruitment of RNA polymerase II at the locus. The findings uncover theWUSchromatin loop as another regulatory mechanism controllingWUSexpression, and also shed light on the factors required for chromatin conformation change and their recruitment. |
| 英文摘要: | WUSCHEL(WUS) is critical for plant meristem maintenance and determinacy in Arabidopsis, and the regulation of its spatiotemporal expression patterns is complex. We previously found that AGAMOUS (AG), a key MADS‐domain transcription factor in floral organ identity and floral meristem determinacy, can directly suppressWUS expression through the recruitment of the Polycomb group (PcG) protein TERMINAL FLOWER2 (TFL2, also known as LIKE HETEROCHROMATIN PROTEIN1, LHP1) at theWUS locus; however, the mechanism by whichWUSis repressed remains unclear. Here, using chromosome conformation capture (3C) and chromatin immunoprecipitation 3C, we found that two specific regions flanking theWUSgene body bound by AG and TFL2 form a chromatin loop that is directly promoted by AG during flower development in a manner independent of the physical distance and sequence content of the intervening region. Moreover, AG physically interacts with TFL2, and TFL2 binding to the chromatin loop is dependent on AG. Transgenic and CRISPR/Cas9‐edited lines showed that theWUSchromatin loop represses gene expression by blocking the recruitment of RNA polymerase II at the locus. The findings uncover theWUSchromatin loop as another regulatory mechanism controllingWUSexpression, and also shed light on the factors required for chromatin conformation change and their recruitment. |
| 刊物名称: | The Plant Journal |
| 英文刊物名称: | The Plant Journal |
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| 其它备注: | Lin Guo, Xiuwei Cao, Yuhao Liu, Jun Li, Yongpeng Li, Dongming Li, Ke Zhang, Caixia Gao, Aiwu Dong, Xigang Liu. A Chromatin Loop Represses WUSCHEL Expression in Arabidopsis. The Plant Journal. DOI:10.1111/tpj.13921 |
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