The Plant Journal
Abstract
Gliadins are a major component of wheat seed proteins. However, the complex homoeologousGli‐2loci (Gli‐A2,‐B2and‐D2) that encode the α‐gliadins in commercial wheat are still poorly understood. Here we analyzed theGli‐D2locus of Xiaoyan 81 (Xy81), a winter wheat cultivar. A total of 421.091kb of theGli‐D2sequence was assembled from sequencing multiple bacterial artificial clones, and 10 α‐gliadin genes were annotated. Comparative genomic analysis showed that Xy81 carried only eight of the α‐gliadin genes of the D genome donorAegilops tauschii, with two of them each experiencing a tandem duplication. A mutant line lackingGli‐D2(DLGliD2) consistently exhibited better breadmaking quality and dough functionalities than its progenitor Xy81, but without penalties in other agronomic traits. It also had an elevated lysine content in the grains. Transcriptome analysis verified the lack ofGli‐D2α‐gliadin gene expression in DLGliD2. Furthermore, the transcript and protein levels of protein disulfide isomerase were both upregulated in DLGliD2 grains. Consistent with this finding, DLGliD2 had increased disulfide content in the flour. Our work sheds light on the structure and function ofGli‐D2in commercial wheat, and suggests that the removal ofGli‐D2and the gliadins specified by it is likely to be useful for simultaneously enhancing the end‐use and health‐related traits of common wheat. Because gliadins and homologous proteins are widely present in grass species, the strategy and information reported here may be broadly useful for improving the quality traits of diverse cereal crops.
| 论文编号: | DOI:10.1111/tpj.13956 |
| 论文题目: | Analysis of the Gli‐D2 Locus Identifies A Genetic Target for Simultaneously Improving the Breadmaking and Health‐related Traits of Common Wheat |
| 英文论文题目: | Analysis of the Gli‐D2 Locus Identifies A Genetic Target for Simultaneously Improving the Breadmaking and Health‐related Traits of Common Wheat |
| 第一作者: | Da Li, Huaibing Jin, Kunpu Zhang, Zhaojun Wang, Faming Wang, Yue Zhao, Naxin Huo, Xin Liu, Yong Q. Gu, Daowen Wang, Lingli Dong |
| 英文第一作者: | Da Li, Huaibing Jin, Kunpu Zhang, Zhaojun Wang, Faming Wang, Yue Zhao, Naxin Huo, Xin Liu, Yong Q. Gu, Daowen Wang, Lingli Dong |
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| 发表年度: | 2018-06-07 |
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| 摘要: | Gliadins are a major component of wheat seed proteins. However, the complex homoeologousGli‐2loci (Gli‐A2,‐B2and‐D2) that encode the α‐gliadins in commercial wheat are still poorly understood. Here we analyzed theGli‐D2locus of Xiaoyan 81 (Xy81), a winter wheat cultivar. A total of 421.091kb of theGli‐D2sequence was assembled from sequencing multiple bacterial artificial clones, and 10 α‐gliadin genes were annotated. Comparative genomic analysis showed that Xy81 carried only eight of the α‐gliadin genes of the D genome donorAegilops tauschii, with two of them each experiencing a tandem duplication. A mutant line lackingGli‐D2(DLGliD2) consistently exhibited better breadmaking quality and dough functionalities than its progenitor Xy81, but without penalties in other agronomic traits. It also had an elevated lysine content in the grains. Transcriptome analysis verified the lack ofGli‐D2α‐gliadin gene expression in DLGliD2. Furthermore, the transcript and protein levels of protein disulfide isomerase were both upregulated in DLGliD2 grains. Consistent with this finding, DLGliD2 had increased disulfide content in the flour. Our work sheds light on the structure and function ofGli‐D2in commercial wheat, and suggests that the removal ofGli‐D2and the gliadins specified by it is likely to be useful for simultaneously enhancing the end‐use and health‐related traits of common wheat. Because gliadins and homologous proteins are widely present in grass species, the strategy and information reported here may be broadly useful for improving the quality traits of diverse cereal crops. |
| 英文摘要: | Gliadins are a major component of wheat seed proteins. However, the complex homoeologousGli‐2loci (Gli‐A2,‐B2and‐D2) that encode the α‐gliadins in commercial wheat are still poorly understood. Here we analyzed theGli‐D2locus of Xiaoyan 81 (Xy81), a winter wheat cultivar. A total of 421.091kb of theGli‐D2sequence was assembled from sequencing multiple bacterial artificial clones, and 10 α‐gliadin genes were annotated. Comparative genomic analysis showed that Xy81 carried only eight of the α‐gliadin genes of the D genome donorAegilops tauschii, with two of them each experiencing a tandem duplication. A mutant line lackingGli‐D2(DLGliD2) consistently exhibited better breadmaking quality and dough functionalities than its progenitor Xy81, but without penalties in other agronomic traits. It also had an elevated lysine content in the grains. Transcriptome analysis verified the lack ofGli‐D2α‐gliadin gene expression in DLGliD2. Furthermore, the transcript and protein levels of protein disulfide isomerase were both upregulated in DLGliD2 grains. Consistent with this finding, DLGliD2 had increased disulfide content in the flour. Our work sheds light on the structure and function ofGli‐D2in commercial wheat, and suggests that the removal ofGli‐D2and the gliadins specified by it is likely to be useful for simultaneously enhancing the end‐use and health‐related traits of common wheat. Because gliadins and homologous proteins are widely present in grass species, the strategy and information reported here may be broadly useful for improving the quality traits of diverse cereal crops. |
| 刊物名称: | The Plant Journal |
| 英文刊物名称: | The Plant Journal |
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| 其它备注: | Da Li, Huaibing Jin, Kunpu Zhang, Zhaojun Wang, Faming Wang, Yue Zhao, Naxin Huo, Xin Liu, Yong Q. Gu, Daowen Wang, Lingli Dong. Analysis of the Gli‐D2 Locus Identifies A Genetic Target for Simultaneously Improving the Breadmaking and Health‐related Traits of Common Wheat. The Plant Journal. DOI:10.1111/tpj.13956 |
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